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Nestin, A New Marker, Expressed in Müller Cells Following Retinal Injury

Published online by Cambridge University Press:  02 December 2014

Liping Xue ,
Peng Ding ,
Libo Xiao ,
Min Hu  and
Zhulin Hu
Liping Xue*
Affiliation:
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou Deparment of Ophthalmology, Yunnan NO.2 Provincial People's Hospital
Peng Ding
Affiliation:
Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming, PR China
Libo Xiao
Affiliation:
Deparment of Ophthalmology, Yunnan NO.2 Provincial People's Hospital
Min Hu
Affiliation:
Deparment of Ophthalmology, Yunnan NO.2 Provincial People's Hospital
Zhulin Hu
Affiliation:
Deparment of Ophthalmology, Yunnan NO.2 Provincial People's Hospital
*
Department of Ophthalmology,, unnan NO.2 Provincial People’s Hospital, 176 Qingnian Rd, Kunming, PR China 650031.
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Abstract

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Purpose:

To investigate whether nestin would be a useful marker for retinal injury and also to ascertain a better understanding of the roles of Müller cells in the injured retina by the use of damaged rat retina.

Methods:

A total of 33 adult female Wistar rats were used in this study. Three were used as controls and the remaining as retinal injury modes (6 for hypoxia; 15 for experimental glaucoma and 9 for optic nerve transection). Double immunofluorescence labeling was carried out between nestin and glutamine synthetase (GS), and between glial fibrillary acidic protein (GFAP) and GS antisera in normal and pathological retinae.

Results:

The results showed that there were no nestin nor GFAP staining in mature Müller cells of the normal retina. A major finding was that nestin expression was induced in Müller cells subjected to hypoxia, glaucoma and optic nerve transection.

Conclusions:

These results suggest that nestin as well as GFAP (even more sensitive than GFAP) are useful and reliable biomarkers for retinal damage. The more intense expression of nestin, GFAP and GS in the end-feet of Müller cells suggest that they may help to maintain the retinal structural integrity and to enhance functional recovery in various retinal diseases.

Résumé:

RÉSUMÉ:Objectif:

Le but de l’étude était d’examiner si la nestine pourrait constituer un marqueur utile de lésion rétinienne et d’acquérir une meilleure compréhension des rôles des cellules de Müller dans la rétine qui a subi une lésion en utilisant la rétine de rat endommagée comme modèle.

Méthodes:

Trente–trois ratsWistar femelles ont été utilisés dans cette étude. Trois rats ont servi de contrôles et 30 ont servi de modèles de lésions rétiniennes (6 de lésion par hypoxie, 15 de glaucoma expérimental et 9 de section du nerf optique). Un double marquage immunofluorescent a été réalisé dans la rétine normale et la rétine pathologique, d’une part pour la nestine et la glutamine synthétase (GS) et d’autre part pour la protéine acide fibrillaire gliale (GFAP) et l’antisérum GS.

Résultats:

Il n’y avait pas de nestine ou de GFAP dans les cellules de Müller matures de la rétine normale. L’expression de la nestine était induite dans les cellules de Müller soumises à l’hypoxie, au glaucome et à la section du nerf optique.

Conclusions:

Ces résultats suggèrent que la nestine, qui est plus sensible que la GFAP, de même que la GFAP sont des marqueurs utiles et fiables du dommage rétinien. L’expression plus intense de la nestine, de la GFAP et de la GS dans les pieds astrocytaires des cellules gliales de Müller suggère qu’il est possible qu’elles aident à maintenir l’intégrité de la structure rétinienne et à favoriser la guérison fonctionnelle dans plusieurs maladies rétiniennes.

Type
Original Article
Information
Canadian Journal of Neurological Sciences , Volume 37 , Issue 5 , September 2010 , pp. 643 - 649
Copyright
Copyright © The Canadian Journal of Neurological 2010

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