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SNP in intron 5 of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene and its genetic effects on important economic traits in geese

  • Liang Zhong (a1) (a2), Chen Hong-Quan (a1) (a2), Huang Hua-Yun (a1), Zha Li-Sha (a1) and Chen Hua (a1)...
Abstract

Intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its single nucleotide polymorphism (SNP) was tested by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in Wanxi White geese (WW) (Anser anser). The carcass, meat quality and feather-down traits of 116 geese from WW males×Sichuan White goose (A. anser) females were assessed and analysed in association with SNP. The results showed that the sequence length of intron 5 of the HMGR gene in geese was 704 bp, and the homology between goose and chicken was 44.0%. A SNP site was tested in intron 5 by PCR-SSCP, and frequencies of alleles A and B at the SNP site were 0.7716 and 0.2284, respectively. The effects of genotype AA were significant, not only on an increase of chest muscle weight, stomach gland weight (P<0.05), fibre diameter of chest muscle and 1000-down weight (P<0.01), but also on a decrease of down water-holding percentage and pH of chest muscle 45 min after slaughterP<0.05). The effect of genotype AB was in the opposite direction to that of genotype AA.

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*Corresponding author. E-mail: chqchq@ahau.edu.cn
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Chinese Journal of Agricultural Biotechnology
  • ISSN: 1479-2362
  • EISSN: 1479-2370
  • URL: /core/journals/chinese-journal-of-agricultural-biotechnology
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