Canine Piroplasmosis. VI. Studies on the Morphology and Life-History of the Parasite

In the present paper we describe the results of further investigations on the life-history of Piroplasma canis in the blood of the dog. In our last paper (x. 1906) we described and figured the movements of the parasite and the mode of multiplication in the dog's blood, and since that time we have confirmed most of our previous observations, and added further facts. The technique of these examinations was fully explained in that paper (p. 604), and it is only necessary to state here that all our later observations on living blood have been made at a temperature of 35°—40°C. The drops of blood were mounted on clean glass slides and cover-glasses kept at this temperature, and were placed as rapidly as possible on the stage of a microscope kept at a similar temperature in a Nuttall's thermostat and examined under a oil immersion lens. In fact we have endeavoured to make our observations in such a manner that the blood should be altered as little as possible.