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Identification of human and bovine rotavirus serotypes by polymerase chain reaction

Published online by Cambridge University Press:  15 May 2009

K. Taniguchi ,
F. Wakasugi ,
Y. Pongsuwanna ,
T. Urasawa ,
S. Ukae ,
S. Chiba  and
S. Urasawa
K. Taniguchi
Affiliation:
Department of Hygiene, Sapporo Medical College, Sapporo 060, Japan
F. Wakasugi
Affiliation:
Department of Hygiene, Sapporo Medical College, Sapporo 060, Japan
Y. Pongsuwanna
Affiliation:
Virus Research Institute, National Institute of Health, Nonthaburi, Thailand
T. Urasawa
Affiliation:
Department of Hygiene, Sapporo Medical College, Sapporo 060, Japan
S. Ukae
Affiliation:
Department of Pediatrics, Sapporo Medical College, Sapporo 060, Japan
S. Chiba
Affiliation:
Department of Pediatrics, Sapporo Medical College, Sapporo 060, Japan
S. Urasawa
Affiliation:
Department of Hygiene, Sapporo Medical College, Sapporo 060, Japan
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Summary

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The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82·6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 303 - 312
Copyright
Copyright © Cambridge University Press 1992

References

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