Skip to main content Accessibility help
×
Home

Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction

  • R. P. Hobson (a1), A. Williams (a1), K. Rawal (a1), T. H. Pennington (a1) and K. J. Forbes (a1)...

Summary

A PCR-based method of detecting Haemophilus influenzae in cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to an H. influenzae gene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains of H. influenzae were detected. One particular strain was detected in a third of the base members.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction
      Available formats
      ×

Copyright

References

Hide All
1.Tzabar, Y, Pennington, TH. The population structure and transmission of Escherichia coli in an isolated human community; studies on an Antarctic base. Epidemiol Infect 1991; 107: 537–42.
2.Maggs, AF, Pennington, TH. Temporal study of staphylococcal species on the skin of human subjects in isolation and clonal analysis of Staphylococcus capitis by sodium dodecyl sulphate–polyacrylamide gel electrophoresis. J Clin Microbiol 1989; 27: 2627–32.
3.Lacoux, PA, Jordens, JZ, Fenton, CM, Guiney, M, Pennington, TH. Characterization of enterococcal isolates by restriction enzyme analysis of genomic DNA. Epidemiol Infect 1992; 109: 6980.
4.Kerr, GRD, Forbes, KJ, Pennington, TH. An analysis of the diversity of Haemophilus parainfluenzae in the adult human respiratory tract by genomic DNA fingerprinting. Epidemiol Infect 1993; 111: 8998.
5.Shapiro, E, Ward, JI. The epidemiology and prevention of disease caused by Haemophilus influenzae type b. Epidemiol Rev 1991; 13: 113–42.
6.Lester, A, Pedersen, PB. Serious systemic infection caused by non-encapsulated Haemophilus influenzas biotype III in an adult. Scand J Infect Dis 1991; 23: 111–3.
7.Forbes, KJ, Morgan, MG, Williams, A, Gould, IM. Molecular epidemiology of invasive Haemophilus influenzae in Scotland. Communicable Diseases and Environmental Health in Scotland Weekly Report 1994; 28 (19): 49.
8.Duim, B, Dankert, J, Jansen, H, van Alphen, L. Genetic analysis of the diversity in outer membrane protein P2 of non-encapsulated Haemophilus influenzae. Microbial Pathogenesis 1993; 14: 451–62.
9.Forbes, KJ, Bruce, KD, Ball, A, Pennington, TH. Variation in length and sequence of porin (ompP2) alleles of non-capsulate Haemophilus influenzae. Mol Microbiol 1992; 6: 2107–12.
10.Hansen, EJ, Hasemann, C, Clausell, A et al. , Primary structure of the porin protein of Haemophilus influenzae type b determined by nucleotide sequence analysis. Infect Immun 1989; 57: 1100–7.
11.Munson, R, Tolan, RW. Molecular cloning, expression and primary sequence of outer membrane protein P2 of Haemophilus influenzae type b. Infect Immun 1989; 57: 8894.
12.Kocagoz, T, Yilmaz, E, Ozkara, S et al. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure. J Clin Microbiol 1993; 31: 1435–8.
13.Folguiera, L, Delgado, R.Palenque, E, Noriega, A. Detection of Mycobacterium tuberculosis DNA in clinical samples by using a simple lysis method and polymerase chain reaction. J Clin Microbiol 1993; 31: 1019–21.
14.Roosendaal, R, Walboomers, JMM, Veltmann, OR et al. Comparison of different primer sets for detection of Chlamydia trachomatis by the polymerase chain reaction. J Med Microbiol 1993; 38: 426–33.
15.Tamburrini, E, Mencarini, P, De Luca, A et al. Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison with immunofluorescence in bronchoalveolar lavage specimens. J Med Microbiol 1993; 38: 449–53.
16.Luneberg, E, Jensen, JS, Frosch, M. Detection of Mycoplasma pneumonias by polymerase chain reaction and nonradioactive hybridization in microtiter plates. J Clin Microbiol 1993; 31: 1088–94.
17.Nguyen, AH, Engstrand, L, Genta, RM, Graham, DY, El-Zaatari, FAK. Detection of Helicobacter pylori in dental plaque by reverse transcription–polymerase chain reaction. J Clin Microbiol 1993; 31: 783–7.
18.Song, J, Cho, H, Park, MY et al. Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction. J Clin Microbiol 1993; 31: 1439–43.
19.Johnson, JD.Butcher, PD.Savva, D, Holliman, RE. Application of the polymerase chain reaction to the diagnosis of human toxoplasmosis. J Infect 1993; 26: 147–58.
20.Van Ketel, RJ, De Wever, B.Van Alphen, L. Detection of Haemophilus influenzae in cerebrospinal fluids by polymerase chain reaction DNA amplification. J Med Microbiol 1990:33:271–6.
21.Forbes, KJ.Bruce, KD.Jordens, JZ, Ball, A, Pennington, TH. Rapid methods in bacterial DNA fingerprinting. J Gen Microbiol 1991; 137: 2051–8.
22.Edwards, U, Rogall, T.Blocker, H.Emde, M, Bottger, E. Isolation and direct complete nucleotide determination of entire genes. Characterisation of a gene coding for 16S ribosomal RNA. Nucleic Acid Res 1989; 17: 7843–53.
23.Sambrook, J.Fritsch, EF, Maniatis, T. Molecular cloning; a laboratory manual. Cold Spring Harbor: Cold Spring Habor laboratory, 1989.
24.Bjourson, AJ, Cooper, JE. Band-stab PCR: a simple technique for the purification of individual PCR products. Nucleic Acid Res 1992; 20: 4675.
25.Sikkema, DJ.Murphy, TF. Molecular analysis of the P2 porin protein of nontypable Haemophilus influenzae. Infect Immun 1992; 60: 5204–11.
26.Noble, WO, Somerville, DA. Major problems in dermatology. In: Microbiology of human skin, vol 2. Philadelphia: W. B. Saunders, 1974; 50–3.
27.Turk, DC, May, JR. Haemophilus influenzae. its clinical importance. 1st edn.English Universities Press Ltd, 1967: 18.
28.Takala, AK, Eskola, J, Leinonen, M et al. Reduction of oropharyngeal carriage of Haemophilus influenzae type B (Hib) in children immunized with an Hib conjugate vaccine. J Infect Dis 1991; 164: 982–6.
29.Masters, PL, Brumfitt, W, Mendez, RL. Bacterial flora of the upper respiratory tract in Paddington families, 1952–4. B M J 1958; 1200–5.
30.Kuklinska, D, Kilian, M. Relative proportions of Haemophilus species in the throat of healthy children and adults. Eur J Clin Microbiol 1984; 3: 249–52.
31.Turk, DC, May, JR. Haemophilus influenzae, its clinical importance. 1st edn.English Universities Press, 1967: 41—2.

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed