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Prevalence and clinical correlations of genetic subtypes of Giardia lamblia in an urban setting

  • A. S. BREATHNACH (a1), T. D. McHUGH (a2) and P. D. BUTCHER (a1)
Abstract
SUMMARY

The clinical significance of different genetic subtypes or assemblages of Giardia lamblia is uncertain. Cases of giardiasis in south-west London between 1999 and 2005 were studied, comparing molecular-typing results with clinical and epidemiological findings from routine surveillance. We identified 819 cases, of whom 389 returned surveillance questionnaires. A subset of 267 faecal samples was submitted for typing by sequencing of the triose phosphate isomerase (tpi) and ribosomal RNA genes, and/or a separate duplex PCR of the tpi gene. Typing was successful in 199 (75%) samples by at least one of the molecular methods. Assemblage A accounted for 48 (24%) samples and Assemblage B for 145 (73%); six (3%) were mixed. Both assemblages had similar seasonality, age distribution and association with travel. Clinical features were available for 59 successfully typed cases: both assemblages caused similar illness, but Assemblage A was significantly more frequently associated with fever than Assemblage B.

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Corresponding author
*Author for correspondence: Dr A. S. Breathnach, Centre for Infection, Division of Cellular and Molecular Medicine, St George's University of London, LondonSW17 0RE, UK. (Email: aodhan.breathnach@stgeorges.nhs.uk)
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3. DT Dennis , Endemic giardiasis in New Hampshire: a case-control study of environmental risks. Journal of Infectious Diseases 1993; 167: 13911395.

6. RM Hopkins , Ribosomal RNA sequencing reveals differences between the genotypes of giardia isolates recovered from humans and dogs living in the same locality. Journal of Parasitology 1997; 83: 4451.

7. PT Monis , Molecular systematics of the parasitic protozoan Giardia intestinalis. Molecular Biology and Evolution 1999; 16: 11351144.

9. SM Caccio , Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. International Journal for Parasitology 2008; 38: 15231531.

10. PL Ey , JM Darby . VSP417-6, a variant-specific surface protein encoded at a sixth locus within the vsp417 gene subfamily of Giardia intestinalis. International Journal for Parasitology 2002; 32: 425436.

11. P Karanis , PL Ey . Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany. Parasitology Research 1998; 84: 442449.

12. O Franzen , Draft genome sequencing of Giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species? PLoS Pathogens 2009; 5: e1000560.

15. AJ Lawson , Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques. Journal of Applied Microbiology 1997; 83: 375380.

16. EA Meyer . Giardia lamblia: isolation and axenic cultivation. Experimental Parasitology 1976; 39: 101105.

20. F Sanger , S Nicklen , AR Coulson . DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences USA 1977; 74: 54635467.

27. M Lalle , Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. International Journal for Parasitology 2005; 35: 207213.

28. IM Sulaiman , Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis. Emerging Infectious Diseases 2003; 9: 14441452.

29. L Eligio-Garcia , A Cortes-Campos , E Jimenez-Cardoso . Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin. Parasitology Research 2005; 97: 16.

31. LJ Robertson , Application of genotyping during an extensive outbreak of waterborne giardiasis in Bergen, Norway, during autumn and winter 2004. Applied and Environmental Microbiology 2006; 72: 22122217.

34. AF Aydin , Classification of Giardia duodenalis parasites in Turkey into groups A and B using restriction fragment length polymorphism. Diagnostic Microbiology and Infectious Disease 2004; 50: 147151.

35. AS Paintlia Giardia lamblia groups A and B among young adults in India. Clinical Infectious Diseases 1998; 26: 190191.

36. C Read , Correlation between genotype of Giardia duodenalis and diarrhoea. International Journal for Parasitology 2002; 32: 229231.

38. T Gelanew , Molecular characterization of human isolates of Giardia duodenalis from Ethiopia. Acta Tropica 2007; 102: 9299.

39. WL Homan , TG Mank . Human giardiasis: genotype linked differences in clinical symptomatology. International Journal for Parasitology 2001; 31: 822826.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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