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Properties of strains of Escherichia coli belonging to serogroup O 157 with special reference to production of Vero cytotoxins VTl and VT2

  • S. M. Scotland (a1), G. A. Willshaw (a1), H. R. Smith (a1) and B. Rowe (a1)

Fifty-four strains of Escherichia coli belonging to serogroup O 157 were examined for the production of Vero cytotoxins VT1 and VT2, and for other properties such as plasmid content, resistance to antimicrobial agents and colicin production. Twenty-six strains from cases of diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome in humans produced VT. By serum neutralization tests and hybridization with DNA probes for VTl or VT2, three classes were recognized which produced either VTl alone or VT2 alone or both VTl and VT2. These strains were of H type 7 or non-motile. The strains producing VT were sensitive to all the antimicrobial agents tested, and all carried at least one plasmid which had a molecular weight of c.60 × 106. Seven strains of porcine origin and 21 strains of human origin did not produce VT or hybridize with either DNA probe. None of these strains was of H type 7. Of the 21 human VT” strains, 17 were of extraintestinal origin and 18 were of H type 45. Twenty-three of the 28 VT” strains were resistant to at least one antimicrobial agent.

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H. C. Birnboim & J. Doly (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7, 15131523.

A. G. Dean , Y. C. Ching , R. G. Williams & L. B. Harden (1972). Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. Journal of Infectious Diseases 125, 407411.

W. R. Ghansden , M. A. S. Damm , J. D. Anderson , J. E. Carter & H. Lior (1986). Further evidence associating haemolytic uraemic syndrome with infection by Verotoxin-producing Escherichia coli O157.H7. Journal of Infectious Diseases 154, 522524.

M. A. Karmali , M. Petric , C. Lim , P. C. Fleming , G. S. Arbus & H. Lior (1985). The association between idiopathic hemolytic uremic syndrome and infection by Verotoxinproducing Escherichia coli. Journal of Infectious Diseases 151, 775782.

M. A. Neill , J. Agosti & H. Rosen (1985). Hemorrhagic colitis with Escherichia coli O 157. H7 preceding adult hemolytic uremic svndrome. Archives of Internal Medicine 145, 22152217.

J. W. Newland , N. A. Strockbine , S. F. Miller , A. D. O'Brien & R. K. Holmes (1985). Cloning of Shiga-like structural genes from a toxin converting phage of Escherichia coli. Science 230, 179181.

A. D. O'Brien , J. W. Newland , S. F. Miller , R. K. Holmes , H. W. Smith & S. B. Formal (1984). Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226, 694696.

C. H. Pai , R. Gordon , H. V. Sims & L. E. Bryan (1984). Sporadic cases of hemorrhagic colitis associated with Escherichia coli O 157.H 7. Chnical, epidemiologic, and bacteriologic features. Annals of Internal Medicine 101, 738742.

R. S. Remis , K. L. MacDonald , L. W. Rilby , N. Puim , L. G. Wells , B. R. Davis , R. J. Hebert , E. S. Olcott , L. M. Johnson , N. T. Hargrett , P. A. Blake & M. L. Cohen (1984). Sporadic cases of hemorrhagic colitis associated with Escherichia coli O 157.H7. Annals of Internal Medicine 101, 624626.

L. W. Riley , R. S. Remis , S. D. Helgerson , H. B. McGee , J. G. Wells , B. R. Davis , R. J. Hebert , E. S. Olcott , L. M. Johnson , N. T. Hargrett , P. A. Blake & M. L. Cohen (1983). Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308, 681685.

C. A. Ryan , R. V. Tauxe , G. W. Hosek , J. G. Wells , P. A. Stoesz , H. W. McFadden , P. W. Smith , G. F. Wright & P. A. Blake (1986). Escherichia coli O 157.H7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. Journal of Infectious Diseases 154, 631638.

S. M. Scotland , N. P. Day & B. Rowe (1980). Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters 7, 1517.

J. S. Spika , J. E. Parsons , D. Nordenberg , J. G. Wells , R. A. Gunn & P. A. Blake (1986). Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O 157.H7 in a day care center. Journal of Pediatrics 109, 287291.

C. M. Taylor , R. H. R. White , M. H. Winterborn & B. Rowe (1986). Haemolytic-uraemic syndrome: clinical experience of an outbreak in the West Midlands. British Medical Journal 292, 15131516.

G. A. Willshaw , H. R. Smith & E. S. Anderson (1979). Application of agarose gel electrophoresis to the characterization of plasmid DNA in drug-resistant enterobacteria. Journal of General Microbiology 114, 1525.

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