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The reaction of H-substance with rabbit antisera

  • R. G. S. Johns (a1) and J. R. Marrack (a1)
Extract

Quantitative methods have been used in several investigations by Kabat and his colleagues (1945–50) in the study of serological relations of various preparations of blood-group substances. The investigations have taken twoforms: (1) the amounts of antibody nitrogen precipitated from a standard volume of an antiserum by varying amounts of the blood-group substances have been plotted against the amount of blood-group substance added; (2) the hexosamine has been measured in the precipitates formed with antiserum in the zone of antibody excess and the ratio of hexosamine precipitated to hexosamine in the blood-group substance added has been calculated; the amount of methyl-pentose precipitated has also been measured (Kabat, Baer &Knaub, 1949) and the ratio of methyl-pentose precipitated to methyl-pentose added hasbeen calculated.

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References
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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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