Skip to main content Accessibility help
×
×
Home

Serovars of Leptospira isolated from dogs and rodents

  • S. M. SUEPAUL (a1), C. V. F. CARRINGTON (a2), M. CAMPBELL (a1), G. BORDE (a1) and A. A. ADESIYUN (a1)...
Summary

We determined the frequency of isolation of Leptospira from dogs and rodents, the serovars of Leptospira, and the clinical, gross and histological manifestations in dogs with leptospirosis in Trinidad. From dogs, samples of urine, blood and kidney were collected while only kidney and blood samples of trapped rodents were used. Isolates were cultured and serotyped using a panel of 23 international serovars and monoclonal antibodies. The risk factors for leptospirosis were also determined in owned dogs using a standard questionnaire. Of a total of 468 animals investigated for Leptospira, 70 (15·0%) were positive, comprising nine (18·0%) of 50 suspected canine leptospirosis cases, seven (3·4%) of 207 stray dogs and 54 (25·6%) of 211 rodents. The observation that rodents have a statistically (P<0·05, χ2) higher frequency of isolation emphasizes the importance of rodents as reservoirs of leptospirosis in the country. Copenhageni was the predominant serovar found in 100·0% (7/7), 33·3% (2/6) and 68·5% (37/54) of isolates from suspected canine leptospirosis cases, stray dogs and rodents, respectively. Serovars Icterohaemorrhagiae and Canicola, the two serovars present in the commercial vaccines used locally, were detected in one (1·5%) and zero (0·0%) isolates respectively of the 67 tested. Data provided suggest that the apparent vaccine failure may be a consequence of the fact that the predominant serovar (Copenhageni) detected in sick, apparently healthy dogs and in rodents is not contained in the vaccines used locally to protect dogs against canine leptospirosis.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Serovars of Leptospira isolated from dogs and rodents
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Serovars of Leptospira isolated from dogs and rodents
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Serovars of Leptospira isolated from dogs and rodents
      Available formats
      ×
Copyright
Corresponding author
*Author for correspondence: Professor A. A. Adesiyun, School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, Trinidad, West Indies. (Email: Abiodun.Adesiyun@sta.uwi.edu)
References
Hide All
1.WHO. Human leptospirosis: guidance for diagnosis, surveillance and control. World Health Organization Library Cataloguing-in Publication Data, 2003.
2.Brown, CA, et al. Leptospira interrogans serovar Grippotyphosa infection in dogs. Journal of the American Veterinary Medical Association 1996; 209: 12651267.
3.De Fritas, JC, et al. Isolation of Leptospira spp. from dogs, bovine and swine naturally infected. Ciencia Rural, Santa Maria 2004; 34: 853856.
4.Jones, CJ, et al. Bim, a new serovar of Leptospira interrogans isolated from a dog in Barbados. Journal of Clinical Microbiology 1984; 19: 946.
5.Nielsen, JN, et al. Leptospira interrogans serovar Bratislava infection in two dogs. Journal of the American Veterinary Medical Association 1991; 199: 351352.
6.Anderson, JF, et al. Isolation of Leptospira interrogans serovar Grippotyphosa from the skin of a dog. Journal of American Veterinary Medical Association 1993; 203: 15501551.
7.Rossetti, CA, et al. Buenos Aires, a new Leptospira serovar of serogroup Djasiman, isolated from an aborted dog fetus in Argentina. Journal of Veterinary Microbiology 2005; 107: 241248.
8.Ruhl-Fehlert, CI, et al. Clinical, microbiological and pathological observations in laboratory beagle dogs infected with leptospires of serogroup Sejroe. Experimental Toxicological Parasitology 2000; 52: 201207.
9.Faine, S, et al. Leptospira and Leptospirosis, 2nd edn. Armadale, Australia: CRC Press, 1994.
10.Ananda, KJ, et al. Diagnosis and treatment of leptospirosis in a dog – a case report. Veterinary World 2008; 1: 278279.
11.Adler, B, de la Pena Moctezuma, A. Leptospira and leptospirosis. Veterinary Microbiology 2009. Published online 13 March 2009; doi:10.1016/j.vetmic.2009.03.02.
12.Vijayachari, P, Sugunan, AP, Shriram, AN. Leptospirosis: an emerging global public health problem. Journal of Biosciences 2008; 33: 557569.
13.Vanasco, NB, et al. Associations between leptospiral infection and seropositivity in rodents and environmental characteristics in Argentina. Journal of Preventive Veterinary Medicine 2003; 60: 227235.
14.Levett, PN. Leptospirosis. Clinical Microbiology Reviews 2001; 14: 296326.
15.Okewole, EA, Ayoola, MO. Seroprevalence of leptospiral serovars other than Canicola and Icterohaemorrhagiae in dogs in the Southwestern Nigeria. Veterinarski Arhiv 2009; 79: 8796.
16.Everard, COR, et al. Leptospirosis in dogs and cats on the island of Trinidad: West Indies. International Journal of Zoonoses 1979; 6: 3340.
17.Everard, COR, Green, AE. Leptospirosis in Trinidad and Grenada, with special reference to the mongoose. Transactions of the Royal Society of Tropical Medicine and Hygiene 1976; 70: 5761.
18.Adesiyun, AA, et al. Sero-epidemiology of canine leptospirosis in Trinidad: serovars, implications for vaccination and public health. Journal of Veterinary Medicine Series B. Infectious Diseases Veterinary Public Health 2006; 53: 9199.
19.Tabata, R, et al. Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe. Brazilian Journal of Medical and Biological Research 2002; 33: 265268.
20.Wixson, SK, et al. The effects of pentobarbital, fentanyl-droperidol, ketamine-xylazine and ketamine-diazepam on core and surface temperature regulation in adult male rats. Laboratory Animal Science 1987; 37: 736746.
21.KIT. International course on laboratory methods for the diagnosis of leptospirosis. Koninklijk Instituut voor de Tropen/Royal Tropical Institute (KIT), Biomedical Research Laboratory, Amsterdam, The Netherlands.
22.Korver, H, et al. Classification of serovars of the Icterohaemorrhagiae serogroup by monoclonal antibodies. Israeli Journal of Veterinary Medicine 1988; 44: 1518.
23.Po-Chang, L, et al. Demographic and clinical features of leptospirosis: three-year experience in central Taiwan. Journal of Microbiology and Immunology of Infections 2006; 41: 145150.
24.Wagenaar, J, et al. Comparison of polymerase chain reaction assays with bacteriological culture, immunofluorescence, and nucleic acid hybridization for detection of Leptospira borgpetersenii serovar hardjo in urine of cattle. American Journal of Veterinary Research 2000; 61: 316320.
25.Jones, TC, Hunt, RD, King, NW. Veterinary Pathology, 6th illustrated edn. Maryland, USA: Wiley-Blackwell, 1998, pp. 1392.
26.Meeyam, T, et al. Seroprevalence and risk factors associated with leptospirosis in dogs. Southeast Asian Journal of Tropical Medicine and Public Health 2006; 37: 148153.
27.Ward, MP. Clustering of reported cases of leptospirosis among dogs in the United States and Canada. Journal of Preventive Medicine 2002; 56: 215226.
28.Ward, WP, Glickman, LT, Guptill, LF. Prevalence of and risk factors for leptospirosis among dogs in the United States and Canada. Journal of the American Veterinary Medical Association 2002; 220: 5358.
29.Yasuda, PH, et al. The isolation from stray dogs in the city of Sao Paulo, Brazil. International Journal of Zoonoses 1980; 7: 131134.
30.Cho, MK, et al. Infection rate of Leptospira interrogans in the field rodent, Apodemus Agrarius, in Korea. Epidemiology of Infectious Diseases 1998; 121: 685690.
31.Levett, PN, et al. Surveillence of leptospiral carriage by feral rats in Barbados. West Indian Medical Journal 1998; 47: 1517.
32.Collares-Pereira, M, et al. Rodents and Leptospira transmission risk in Terceira island (Azores). European Journal of Epidemiology 2004; 16: 11511157.
33.Sunbul, M, et al. Rattus norvegicus acting as reservoir of Leptospira interrogans in the Middle Black Sea region of Turkey, as evidenced by PCR and presence of serum antibodies to Leptospira strains. Scandinavian Journal of Infectious Diseases 2001; 33: 896–398.
34.Ahmed, N, et al. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Annals of Clinical Microbiology and Antimicrobials 2006; 5: 10.
35.Thaipadungpanit, J, et al. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Neglected Tropical Diseases 2007; 1: e56.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed