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    Kim, Tae Jung and Lee, Jae Il 2006. The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates. Journal of Veterinary Science, Vol. 7, Issue. 3, p. 277.

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    Siba, P. M. Pethick, D. W. and Hampson, D. J. 1996. Pigs experimentally infected with Serpulina hyodysenteriae can be protected from developing swine dysentery by feeding them a highly digestible diet. Epidemiology and Infection, Vol. 116, Issue. 02, p. 207.

    Witters, Nancy A. and Duhamel, Gerald E. 1996. Cell membrane permeability and mitochondrial dysfunction-inducing activities in cell-free supernatants from Serpulina hyodysenteriae serotypes 1 and 2. Comparative Immunology, Microbiology and Infectious Diseases, Vol. 19, Issue. 3, p. 233.

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Slide-agglutination for rapid serological typing of Treponema hyodysenteriae

  • D. J. Hampson (a1)
  • DOI:
  • Published online: 19 October 2009

A slide agglutination (SA) test was developed to determine the serogroup of isolates of Treponema hyodysenteriae of serogroups A to F. Rabbit antisera which are normally used for serogrouping T. hyodysenteriae in an agarose gel doublediffusion precipitation test (AGDP) were not suitable for SA because they agglutinated isolates from more than one serogroup. The agglutination reaction was made serogroup-specific by cross-absorbing the typing sera for serogroups A to F with whole treponemes from the other 5 of these 6 serogroups of T. hyodysenteriae. The absorbed sera were reacted in slide agglutination tests with 33 isolates of T. hyodysenteriae and with four non-T. hyodysenteriae intestinal spirochaetes. None of the non-T. hyodysenteriae isolates agglutinated, but 27 of the 33 isolates of T. hyodysenteriae did. The results for 26 of the 27 agglutination reactions agreed with the serogroup as determined in AGDP. One of the 6 isolates of T. hyodysenteriae which failed to react in slide agglutination was of serogroup B, 1 of serogroup D, 1 each were from new serogroups G, H and I, and 1 was untypable in AGDP.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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