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Survival of Salmonella eastbourne and Salmonella typhimurium in milk chocolate prepared with artificially contaminated milk powder

Published online by Cambridge University Press:  15 May 2009

S. K. Tamminga ,
R. R. Beumer ,
E. H. Kampelmacher  and
F. M. van Leusden
S. K. Tamminga
Affiliation:
Laboratory for Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands
R. R. Beumer
Affiliation:
Laboratory for Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands
E. H. Kampelmacher
Affiliation:
Laboratory for Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands
F. M. van Leusden
Affiliation:
Laboratory for Zoonoses and Food Microbiology, National Institute of Public Health, Bilthoven, The Netherlands
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Milk chocolate mass containing salmonellas was prepared by mixing artificially contaminated milk powder with the other ingredients at a temperature of about 40 °C. From this mass bars were made. Two series were prepared, with S. eastbourne and S. typhimurium respectively. The number of surviving salmonellas was counted after various periods of storage, up to 19 months. S. eastbourne was reduced in numbers during 19 months from an initial count of ca. 3 × 104 to ca. 3 × 102 per 100 g of chocolate. S. typhimurium died off more rapidly, and was not detectable in about 55 g after 15 months, in spite of an initial count of ca. 105 per 100g.

In these experiments the salmonellas in the milk powder had had to survive the spraying procedure and the adverse conditions in the dried powder. This may be the reason why S. eastbourne showed a distinctly better survival on storage than the same serotype showed in previous experiments in which the organism was added as a broth culture to the chocolate mix. With S. typhimurium, however, such a difference was hardly detectable.

Possible explanations of these results are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 79 , Issue 3 , December 1977 , pp. 333 - 337
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Christian, J. H. B. & Stewart, B. J. (1973). Survival of Staphylococcus aureus and Salmonella newport in dried foods, as influenced by water activity and oxygen. In B. C. Hobbs & J. H. B. Christian (eds), The Microbiological Safety of Foods. Proc. VIIIth Intern. Symp. Food Microbiology,Reading, England,September 1972,Academic Press, London, New York, pp. 107–19.Google Scholar
Craven, P. C., Mackel, D. C., Baine, W. B., Barker, W. H., Gangarosa, E. J., Goldfield, M., Rosenfeld, H., Altman, R., Lachapelle, G., Davies, J. W. & Swanson, R. C. (1975). International outbreak of Salmonella eastbourne infection traced to contaminated chocolate. Lancet i, 788–93.CrossRefGoogle Scholar
Edel, W. & Kampelmacher, E. H. (1969). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297–306.Google ScholarPubMed
Edel, W. & Kampelmacher, E. H. (1973). Comparative studies on the isolation of ‘sublethally injured’ salmonellae in nine European laboratories. Bulletin of the World Health Organization 48, 167–74.Google ScholarPubMed
LiCari, J. J. & Potter, N. N. (1970). Salmonella survival during spray drying and subsequent handling of skim milk powder. III. Effects of storage temperature on Salmonella and dried milk properties. Journal of Dairy Science 53, 877–82.CrossRefGoogle Scholar
Tamminga, S. K., Beumer, R. R., Kampelmacher, E. H. & Van Leusden, F. M. (1976). Survival of Salmonella eastbourne and Salmonella typhimurium in chocolate. Journal of Hygiene 76, 41–7.CrossRefGoogle ScholarPubMed