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The epidemiology and molecular characterization of methicillin-resistant staphylococci sampled from a healthy Jordanian population

  • A. G. AL-BAKRI (a1), H. AL-HADITHI (a1), V. KASABRI (a2), G. OTHMAN (a1), A. KRIEGESKORTE (a3) and K. BECKER (a3)
  • DOI:
  • Published online: 23 January 2013

The prevalence of natural carriage and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7·5% and the majority (81%) of MRSA harboured the resistance element SCCmec type IVe and were of a novel spa type t9519 (76%); other significant spa gene types were t223 (14·7%) and t044 (5·9%). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured the pvl gene. MR-CoNS carriage was 54·2% and these isolates were characterized by single, double and untypable SCCmec elements, with Staphylococcus epidermidis SCCmec type IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS + MRSA, three shared SCCmec type IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

Corresponding author
*Author for correspondence: Dr A. G. Al-Bakri, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The University of Jordan, Amman-Jordan. (Email:
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1.T Yamamoto , Community-acquired methicillin-resistant Staphylococcus aureus: community transmission, pathogenesis, and drug resistance. Journal of Infection and Chemotherapy 2010; 16: 225254.

2.T Ito , Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2001; 45: 13231336.

3.E Garza-Gonzalez , Diversity of staphylococcal chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance. Journal of Medical Microbiology 2010; 59: 323329.

4.F Barbier , Methicillin-resistant coagulase-negative staphylococci in the community: high homology of SCCmec IVa between Staphylococcus epidermidis and major clones of methicillin-resistant Staphylococcus aureus. Journal of Infectious Diseases 2010; 202: 270281.

5.RH Deurenberg , EE Stobberingh . The molecular evolution of hospital- and community-associated methicillin-resistant Staphylococcus aureus. Current Molecular Medicine 2009; 9: 100115.

6.T Na'was , J Fakhoury . Nasal carriage of methicillin resistant Staphylococcus aureus by hospital staff in Jordan. Journal of Hospital Infection 1991; 17: 223229.

7.E Al-Zu'bi , S Bdour , AA Shehabi . Antibiotic resistance patterns of mecA-positive Staphylococcus aureus isolates from clinical specimens and nasal carriage. Microbial Drug Resistance 2004; 10: 321324.

8.HI Daghistani , AA Issa , AA Shehabi . Frequency of nasal and wound isolates of Staphylococcus aureus associated with TSST-1 production in Jordanian population. FEMS Immunology and Medical Microbiology 2000; 27: 9598.

9.W Khalil , Methicillin-resistant Staphylococcus aureus ST80-IV clone in children from Jordan. Diagnostic Microbiology and Infectious Disease 2012; 73: 228230.

10.BA Diep , Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Lancet 2006; 367: 731739.

11.GL Archer , DM Niemeyer . Origin and evolution of DNA associated with resistance to methicillin in staphylococci. Trends in Microbiology 1994; 2: 343347.

12.CS Chen , CY Chen , YC Huang . Nasal carriage rate and molecular epidemiology of methicillin-resistant Staphylococcus aureus among medical students at a Taiwanese university. International Journal of Infectious Diseases 2012; 16: e799803.

13.KA Prates , Nasal carriage of methicillin-resistant Staphylococcus aureus in university students. Brazilian Journal of Infectious Diseases 2010; 14: 316318.

14.C von Eiff , Nasal carriage as a source of Staphylococcus aureus bacteremia. New England Journal of Medicine 2001; 344: 1116.

17.C Milheirico , DC Oliveira , H de Lencastre . Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2007; 51: 33743377.

18.C Milheirico , DC Oliveira , H de Lencastre . Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: ‘SCCmec IV multiplex’. Journal of Antimicrobial Chemotherapy 2007; 60: 4248.

19.G Lina , Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clinical Infectious Diseases 1999; 29: 11281132.

20.S Jarraud , Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infection and Immunity 2002; 70: 631641.

21.DC Oliveira , A Tomasz , H de Lencastre . The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: identification of two ancestral genetic backgrounds and the associated mec elements. Microbial Drug Resistance 2001; 7: 349361.

24.MA Borg , Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in invasive isolates from southern and eastern Mediterranean countries. Journal of Antimicrobial Chemotherapy 2007; 60: 13101315.

26.AG Al-Bakri , Y Bustanji , AM Yousef . Community consumption of antibacterial drugs within the Jordanian population: sources, patterns and appropriateness. International Journal of Antimicrobial Agents 2005; 26: 389395.

27.NH Al-Momany , Adherence to international antimicrobial prophylaxis guidelines in cardiac surgery: a Jordanian study demonstrates need for quality improvement. Journal of Managed Care Pharmacy 2009; 15: 262271.

29.AA Alghaithy , Nasal carriage and antibiotic resistance of Staphylococcus aureus isolates from hospital and non-hospital personnel in Abha, Saudi Arabia. Transactions of the Royal Society Tropical Medicine and Hygiene 2000; 94: 504507.

31.JA Otter , GL French . Utility of antimicrobial susceptibility-based algorithms for the presumptive identification of genotypically-defined community-associated methicillin-resistant Staphylococcus aureus at a London teaching hospital. European Journal of Clinical Microbiology and Infectious Disease 2011; 30: 459463.

32.Y Takizawa , A Panton-Valentine leucocidine (PVL)-positive community – acquired methicillin-resistant Staphylococcus aureus (MRSA) strain, another such strain carrying a multiple-drug resistance plasmid, and another more-typical PVL-negative MRSA strains found in Japan. Journal of Clinical Microbiology 2005; 43: 33563363.

33.A Rossney , The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton–Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community – acquired MRSA strains in Ireland. Journal of Clinical Microbiology 2007; 45: 25542563.

34.SJ Peacock , Virulent combinations of adhesion and toxin genes in natural populations of Staphylococcus aureus. Infection and immunity 2002; 70: 49874996.

35.Z Zong , C Peng , X . Diversity of SCCmec elements in methicillin-resistant coagulase-negative staphylococci clinical isolates PLoS One 2011; 6: e20191.

36.S Ibrahem , Carriage of methicillin-resistant Staphylococci and their SCCmec types in a long-term-care facility. Journal of Clinical Microbiology 2009; 47: 3237.

37.D Lebeaux , Evolution of nasal carriage of methicillin-resistant coagulase-negative staphylococci in a remote population. Antimicrobial Agents and Chemotherapy 2012; 56: 315323.

38.JH Song , Spread of methicillin-resistant Staphylococcus aureus between the community and the hospitals in Asian countries: an ANSORP study. Journal of Antimicrobial Chemotherapy 2011; 66: 10611069.

39.JR Mediavilla , Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA). Current Opinion in Microbiology 2012; 15: 588595.

40.HS Ammerlaan , Eradication of carriage with methicillin-resistant Staphylococcus aureus: effectiveness of a national guideline. Journal of Antimicrobial Chemotherapy. 2011; 66: 24092417.

41.WC Huskins , Intervention to reduce transmission of resistant bacteria in intensive care. New England Journal of Medicine 2011; 364: 14071418.

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Epidemiology & Infection
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