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Virulence factors of Francisella tularensis

  • A. M. Hood (a1)

Summary

The mechanism causing viable Francisella tularensis to lose virulence in aerosols has been investigated. Fully virulent organisms were found to be encapsulated and avirulent organisms from aged aerosols, decapsulated. Capsules were also removed by suspension of F. tularensis in hypertonic sodium chloride. The resulting naked, but viable, organisms were predominantly avirulent for guinea-pigs challenged intraperitoneally. Capsular material and cell walls were found to contain large amounts of lipid, about 50 and 70% (w/w) respectively, and to differ in lipid and sugar composition. Isolated capsular material was not found to contain a lethal toxin for mice or guinea-pigs, or to induce an immunological response in these animals to fully virulent F. tularensis.

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References

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Abdel-Akher, M., Hamilton, J. K. & Smith, F. (1951). The reduction of sugars with sodium borohydride. Journal of the American Chemical Society 73, 4691.
Bobo, R. A. & Eagen, R. G. (1968). Lipids of cell walls of Pseudomonas aeruginosa and Brucella abortus. Canadian Journal of Microbiology 14, 503.
Buddingh, G. L. & Womack, F. C. (1941). Observations on the infection of chick embryos with Bacterium tularense, Brucella and Pasteurella pestis. Journal of Experimental Medicine 74, 213.
Cho, K. Y. & Salton, M. R. J. (1966). Fatty acid composition of bacterial membrane and wall lipids. Biochimica et biophysica acta 116, 73.
Duguid, J. P. (1951). The demonstration of bacterial capsules and slime. Journal of Pathology and Bacteriology 63, 673.
Eigelsbach, H. T., Braum, W. & Herring, R. D. (1951). Studies on the variation of Bacterium tularense. Journal of Bacteriology 61, 557.
Eigelsbach, H. T., Chambers, L. A. & Coriell, L. (1946). Electron microscopy of Bacterium tularense. Journal of Bacteriology 52, 179.
Gordon, M., Donaldson, D. M. & Wright, G. E. (1964). Immunization of mice with irradiated Pasteurella tularensis. Journal of Infectious Diseases 114, 435.
Guss, M. L. (1970). Is Pasteurella tularensis lipopolysaccharide an endotoxin? Bacteriological Proceedings p. 89.
Hambleton, P., Evans, C. G. T., Hood, A. M. & Strange, R. E. (1974). Vaccine potencies of the live vaccine strain of Francisella tularensis and isolated bacterial components. British Journal of experimental Pathology 55, 363.
Hancock, I. C., Humphreys, G. O. & Meadow, P. M. (1970). Characterisation of the hydroxy acids of Pseudomonas aeruginosa 8602. Biochimica et biophysica acta 202, 389.
Harper, G. J., Hood, A. M. & Morton, J. D. (1958). Airborne micro-organisms: a technique for studying their survival. Journal of Hygiene 56, 364.
Hesselbrock, W. & Foshay, L. (1945). The morphology ofBacterium tularense. Journal of Bacteriology 49, 209.
Hood, A. M. (1961). Infectivity of Pasteurella tularensis clouds. Journal of Hygiene, 59, 497.
Kaneshiro, T. & Marr, A. G. (1963). Hydroxy fatty acids of Azotobacter agilis. Biochimica et biophysica acta 70, 271.
Larson, C. L., Bell, J. F. & Owen, C. R. (1954). The development of resistance in mice immunized with soluble antigen derived fromBacterium tularense. Journal of Immunology 73, 221.
May, K. R. (1973). The Collison nebulizer: description, performance and application. Aerosol Science 4, 235.
Mills, R. C., Berthelsen, H., Donaldson, D. & Wilhelm, P. L. (1949). Nutritional requirements of Pasteurella tularense. Bacteriological Proceedings 37.
Moody, M. D. & Downs, C. M. (1955). Studies on Tularaemia. I. The relationship between certain pathogenic and immunogenic properties of variants of Pasteurella tularensis. Journal of Bacteriology 79, 297.
Nutter, J. E. (1971). Antigens of Pasteurella tularensis: Preparative procedures. Applied Microbiology 22, 44.
Nutter, J. E. & Myrvik, Q. M. (1966). In-vitro interactions between rabbit alveolar macro-phages and Pasteurella tularensis. Journal of Bacteriology 92, 645.
O'Hara, H. (1940). Bacterium tularense and 'Yato-Byo' bacteria. Proceedings of the Third International Congress for Microbiology p. 678.
Ormsbee, R. A., Bell, J. F. & Larson, C. L. (1955). Studies on Bacterium tularense antigens I. The isolation, purification and biological activity of antigen preparations fromBacterium tularense. Journal of Immunology 74, 351.
Ormsbee, R. A. & Larson, C. L. (1955). Studies on Bacterium tularense antigens. II. Chemical and physical characteristics of protective antigen preparations. Journal of Immunology 74, 359.
Ota, I. (1936). Morphological studies of Yato-Byo bacteria and Bacterium tularense, especially flagella staining. Kokumin Eisei 13, 207.
Park, C. E. & Berger, L. R. (1967). Fatty acids of extractable and bound lipids of Rhodo-microbium vanniellii. Journal of Bacteriology 93, 230.
Pavlova, I. B., Meshcheryakova, I. S. & Emelyanova, O. S. (1967). Study of the micro-structure of the two geographic races of the Tularaemia bacterium in strains with different degrees of virulence. Journal of Hygiene, Epidemiology, Microbiology and Immunology (Praha) 11, 320.
Pieroni, R. E., Broderick, E. J., Bundeally, A. & Levine, L. (1970). A simple method for the quantitation of submicrogram amounts of bacterial endotoxin. Proceedings of the Society for Experimental Biology and Medicine 133, 790.
Proctor, R. A., White, J. D., Ayala, E. & Canonico, P. G. (1975). Phagocytosis of Francisella tularensis by Rhesus monkey peripheral leukocytes. Infection and Immunity 11, 146.
Salton, M. R. J. (1964). The Bacterial Cell Wall. Amsterdam: Elsevier Publishing Company.
Sawardeker, J. S., Sloneker, J. H. & Jeanes, A. R. (1965). Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Analytical Chemistry 37, 1602.
Sawyer, W. D., Jemski, J. V., Hogge, A. L., Eigelsbach, H. T., Wolft, E. K., Danger-field, H. G., Gochenour, W. S. & Crozier, D. (1966). Effect of aerosol age on the infectivity of airborne Pasteurella tularensis for Macaca mulatta and man. Journal of Bacteriology 91, 2180.
Schlamm, N. A. (1960). Detection of viability in aged or injured Pasteurella tularensis. Journal of Bacteriology 80, 818.
Shepard, C. C. (1959). Non acid fast bacteria and HeLa cells: their uptake and subsequent intracellular growth. Journal of Bacteriology 77, 701.
Shepard, C. C., Ribi, E. & Larson, C. (1954). Electron microscopically revealed structural elements of Bacterium tularense and their in vitro and in vivo role in immunogenic reactions. Journal of Immunology 75, 7.
Stefanye, D. (1961). Lipopolysaccharides of Pasteurella tularensis. Bacteriological Proceedingsz 129.
Stefanye, D., Tresselt, H. B. & Spero, L. (1961). Observations on the behaviour in vitro of Pasteurella tularensis after phagocytosis. Journal of Bacteriology 81, 470
Thorpe, B. D. & Marcus, S. (1964 a). Phagocytosis and intracellular fate of Pasteurella tularensis. I. In vitro studies with rabbit peritoneal mononuclear phagocytes. Journal of Immunology 92, 657.
Thorpe, B. D. & Marcus, S. (1964 b). Phagocytosis and intracellular fate of Pasteurella tularensis. II. In vitro studies with rabbit alveolar and guinea-pig alveolar and peritoneal mononuclear phagocytes. Journal of Immunology 93, 558.
Thorpe, B. D. & Marcus, S. (1965). Phagocytosis and intracellular fate of Pasteurella tularensis. III. In vivo studies with passively transferred cells and sera. Journal of Immunology 94, 578.
Tornabene, T. G. (1973). Lipid composition of selected strains of Yersinia pestis and Yersinia pseudotuberculosis. Biochimica et biophysica acta 306, 173.
Tomcsik, J. (1956). In Bacterial Anatomy (Sixth Symp. of the Society for General Microbiology), p. 41, ed. Spooner, E. T. C. and Stocker, B. A. D.. CambridgeUniversity Press.
Wilkinson, J. F. (1958). The extracellular polysaccharides of bacteria. Bacteriological Reviews 22, 46.
Zimmerman, L. (1969). Permeability of Serratia marcescens to some inorganic salts. Journal of Bacteriology 97, 749.

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