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Nursery hardening of in vitro-produced banana plants

Published online by Cambridge University Press:  08 December 2009

John Charles Robinson
Du Roi Lab., P.O. Box 1147, Letsitele 0885, South Africa
Victor Galán Sáuco
Inst. Canario Investig. Agrar. (ICIA), Apartado Correos 60, 38208 La Laguna, Tenerife, Canary Islands, Spain
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Introduction. This protocol can be used as a guide for people who wish to construct their own nursery. It describes the methods for receiving, transplanting, managing and hardening young banana tissue culture plants after the weaning stage. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the nursery construction, the nursery media, fertiliser and bags, the handling and transplanting of weaned plants into bags (3 steps), and the management schedule for hardening nursery plants (8 steps). Possible troubleshooting is listed. Results. All the characteristics of nursery-hardened plants in bags, ready for transplanting to the field, are formulated.

Research Article
Fruits , Volume 64 , Issue 6 , November 2009 , pp. 383 - 392
© CIRAD, EDP Sciences, 2009

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Robinson, J.C., Galán Sauco, V., Weaning (acclimatization) of in vitro-produced banana plants, Fruits 64 (2009) 325332. CrossRef
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