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Effect of oligochitosan on development of Colletotrichum musae in vitro and in situ and its role in protection of banana fruits

Published online by Cambridge University Press:  06 April 2012

Meng Xiangchun ,
Tang Yanxia ,
Zhang Aiyu ,
Huang Xuemei  and
Zhang Zhaoqi
Meng Xiangchun
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Tang Yanxia
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Zhang Aiyu
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Huang Xuemei
Affiliation:
Coll. Hortic., S. China Agric. Univ., Guangzhou, P.R. China. zqzhang@scau.edu.cn
Zhang Zhaoqi*
Affiliation:
Coll. Hortic., S. China Agric. Univ., Guangzhou, P.R. China. zqzhang@scau.edu.cn
*
Correspondence and reprints
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Abstract

Introduction. Concerns about the potentially harmful effects of fungicides on human health and the environment encourage the search for alternative treatments for perishable fruit postharvest disease control. To this end, the potential use of oligochitosan as a natural antifungal compound to control postharvest anthracnose caused by Colletotrichum musae was investigated in banana fruits from the Cavendish group (genome AAA). Materials and methods. The influence of oligochitosan on the growth of C. musae was determined in vitro by micrographic analysis, while its in situ antifungal activity was monitored in banana fruits that were artificially injury-inoculated with C. musae; the activities of several defense-related enzymes were measured. Results and discussion. Oligochitosan at (4 and 8) g·L–1 markedly inhibited radial mycelial growth of C. musae in vitro. The scanning electron micrograph of C. musae treated with oligochitosan at inhibitory concentrations showed distortion and thinning of the hyphal wall and reduction in fungus colony diameter. Dipping banana fruits in oligochitosan solution at (5 to 20) g·L–1 significantly reduced the diameter of the anthracnose lesion, and 20 g oligochitosan·L–1 almost reached the same inhibitory effect as 0.5 g·L–1 of Sportak®, a synthetic fungicide. Activities of defense-related enzymes such as phenylalanine ammonia-lyase (PAL), β-1, 3-glucanase (GLU) and chitinase (CHT), but not polyphenol oxidase (PPO), increased in banana fruits treated with 0.5 g oligochitosan·L–1. Conclusion. The inhibitory effect of oligochitosan on anthracnose development is due to the combination of a direct antifungal effect on the pathogen and an indirect effect, whereby the activities of defense-related enzymes in the banana fruit were enhanced. To control anthracnose in harvested bananas, treatment with oligochitosan above 20 g·L–1 may substitute the use of synthetic fungicide.

Keywords

ChinaMusa (bananas)postharvest lossesdisease controlbiological controlanthracnosisoligochitosanantifungal propertiesinduced resistanceChineMusa (bananes)perte après récoltecontrôle de maladieslutte biologiqueanthracnoseoligochitosanepropriété antifongiquerésistance induiteChinaMusa (bananos)pérdidas postcosechacontrol de enfermedadescontrol biológicoantracnosisoligoquitosanopropiedades antimicosicasresistencia inducida
Type
Original article
Information
Fruits , Volume 67 , Issue 3 , May 2012 , pp. 147 - 155
Copyright
© 2012 Cirad/EDP Sciences

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