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Fertility of sperm from t/ + mice: evidence that + − bearing sperm are dysfunctional

  • Patricia Olds-Clarke (a1) and Betsy Peitz (a2)
Summary
Summary

Male mice carrying one complete t haplotype transmit it to virtually all offspring. This implies that either the sperm carrying the t haplotype have an advantage in fertilization, or that the sperm not carrying a t haplotype are dysfunctional. To distinguish between these possibilities, epididymal sperm from tw32/ + and congenic + / + males were artificially inseminated into the uterus of T / + females, and the transmission ratio determined from the phenotype of the offspring. After artificial insemination (AI) of sperm from tw32/ + males, the mean ratio was 0·95 (3 experiments, 5 litters), demonstrating that the transmission ratio remains high after AI into the uterus. After AI of a mixture of equal numbers of motile sperm from tw32/ + and congenic + / + males, the mean ratio was 0·22 (6 experiments, 8 litters). These results suggest that sperm carrying a t haplotype from t/ + males are not superior to sperm from + / + males, and therefore imply that sperm not carrying a t haplotype from t/ + males are dysfunctional.

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Bennett D. (1975). The T-locus of the mouse. Cell 6, 441454.
Braden A. W. H. (1972). T-locus in mice: segregation distortion and sterility in the male. In Proceedings of the International Symposium: The Genetics of the Spermatozoon (ed. Beatty R. A. and S. Gluecksohn-Waelsch), pp. 289305. Denmark: Bogtrykkeriet Forum.
Braden A. W. H. and Gluecksohn-Waelsch S. (1958). Further studies on the effect of the T locus in the house mouse on male fertility. Journal of Experimental Zoology 138, 431452.
Buehr M. & McLaren A. (1984). Interlitter variation in progeny of chimaeric male mice. Journal of Reproduction and Fertility 72, 213221.
Edwards R. G. (1955). Selective fertilization following the use of sperm mixtures in the mouse. Nature 175, 215216.
Hammerberg C. & Klein J. (1975). Linkage disequilibrium between H-2 and t complexes in chromosome 17 of the mouse. Nature 258, 296299.
McGrath J. & Hillman N. (1980). The in vitro transmission frequency of the t 12 mutation in the mouse. Journal of Embryology and experimental Morphology 60, 141151.
Mickova M. & Ivanyi P. (1974). Sex-dependent and H-2 linked influence on expressivity of the brachyury gene in mice. Journal of Heredity 65, 369372.
Nadijcka M. & Hillman N. (1980). Differences in cAMP levels in tn- and non-tn-bearing mouse spermatozoa. Biology of Reproduction 11, 11021105.
Olds-Clarke P. & Becker A. (1978). The effect of the T locus on sperm penetration in vivo in the house mouse. Biology of Reproduction 18, 132140.
Seitz A. W. & Bennett D. (1975). Transmission distortion of t-haplotypes is due to interactions between meiotic partners. Nature 313, 143144.
Silver L. M. (1986). Mouse t haplotypes. Ann. Rev. Genet. 19, 179208.
Silver L. M. & Olds-Clarke P. (1984). Transmission ratio distortion of mouse t haplotypes is not a consequence of wild-type sperm degeneration. Developmental Biology 105, 250252.
Tessler S., Carey J. E. & Olds-Clarke P. (1981). Mouse sperm motility affected by factors in the T/t complex. Journal of Experimental Zoology 217, 277285.
Tokuyasu K. T., Peacock W. J. & Hardy R. W. (1977). Dynamics of spermiogenesis in Drosophila melanogaster. VII. effects of Segregation Distorter (SD) chromosome. Journal of Ultrastructure Research 58, 96107.
West J. D., Frels W. I., Papaioannou V. E., Karr J. P. & Chapman V. M. (1977). Development of interspecific hybrids of Mus. Journal of Embryology and experimental Morphology 41, 233243.
Wolf D. & Inoue M. (1976). Sperm concentration dependency in the fertilization and the zonae sperm binding properties of mouse eggs inseminated in vitro. Journal of Experimental Zoology 196, 2738.
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Genetics Research
  • ISSN: 0016-6723
  • EISSN: 1469-5073
  • URL: /core/journals/genetics-research
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