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Gene transfer in Drosophila melanogaster: genetic transformations induced by the DNA of transformed stocks

  • Allen S. Fox (a1), Sheldon D. Parzen (a1), Helen Salverson (a1) and Sei Byung Yoon (a1)
Summary

DNA prepared from transformed stocks of Drosophila melanogaster induces second-step transformations resembling the original. The gross yield of transformants induced by transformed DNA is several times higher than that induced by the original allo-DNA, but much of this high frequency is attributable to a few exceptionally large clusters of transformants among flies treated with transformed DNA. When these large clusters are omitted from the data, the frequency of transformants induced by DNA from transformed stocks is the same as that induced by allo-DNA. The data therefore support the conclusion that the original DNA-induced alterations resulted from the transfer of genetic material capable of indefinite replication.

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Beadle, G. W. & Ephrussi, B. (1937). Development of eye colors in Drosophila: Diffusible substances and their interrelations. Genetics 22, 7686.
Fox, A. S., Duggleby, W. F., Gelbart, W. M. & Yoon, S. B. (1970). DNA-induced transformation in Drosophila: Evidence for transmission without integration. Proceedings of the National Academy of Sciences of the U.S.A. 67, 18341838.
Fox, A. S. & Valencia, J. B. (1973). Cytological detection of structural changes at the sites of DNA-induced transformations in the polytene chromosomes of Drosophila. Genetics 74, Suppl. s.83.
Fox, A. S. & Valencia, J. I. (1975). Gene transfer in Drosophila melanogaster: Cytological alterations in the salivary chromosomes of transformed stocks. Chromosoma 51, 279289.
Fox, A. S. & Yoon, S. B. (1966). Specific genetic effects of DNA in Drosophila melanogaster. Genetics 53, 897911.
Fox, A. S. & Yoon, S. B. (1970). DNA-induced transformation in Drosophila: Locus-specificity and the establishment of transformed stocks. Proceedings of the National Academy of Sciences 67, 16081615.
Fox, A. S., Yoon, S. B. & Gelbart, W. M. (1971 a). DNA-induced transformation in Drosophila: Genetic analysis of transformed stocks. Proceedings of the National Academy of Sciences of the U.S.A. 68, 342346.
Fox, A. S., Yoon, S. B., Duggleby, W. F. & Gelbart, W. M. (1971 b). Genetic transformation in Drosophila. In Informative Molecules in Biological Systems (ed. Ledoux, L. G. H.), pp. 313333. Amsterdam: North-Holland Publishing Co.
Illmensee, K. (1972). Developmental potencies of nuclei from cleavage, preblastoderm, and syncytial blastoderm transplanted into unfertilized eggs of Drosophila melanogaster. Wilhelm Roux Archiv für Entwicklungsmechanik der Organismen 170, 267298.
Lefevre, G. Jr (1969). The eccentricity of vermilion deficiencies in Drosophila melanogaster. Genetics 63, 589600.
Lindsley, D. L. & Grell, E. H. (1968). Genetic Variations of Drosophila melanogaster. Carnegie Institution of Washington, Publication 627.
Mead, C. G. (1964). A deoxyribonucleic acid-associated ribonucleic acid from Drosophila melanogaster. Journal of Biological Chemistry 239, 550554.
Newhall, S. M., Nickerson, D. & Judd, D. B. (1943). Final report of the O.S.A. subcommittee on the spacing of the Munsell colors. Journal of the Optical Society of America 33, 385418.
Okada, M., Kleinman, A. & Schneiderman, H. A. (1974). Restoration of fertility in sterilized Drosophila eggs by transplantation of polar cytoplasm. Developmental Biology 37, 4354.
Schubiger, M. & Schneiderman, H. A. (1971). Nuclear transplantation in Drosophila melanogaster. Nature 230, 185186.
Tobler, J. E. (1975). Dosage compensation and ontogenic expression of suppressed and transformed vermilion flies in Drosophila. Biochemical Genetics 13, 2943.
Yoon, S. B. & Fox, A. S. (1965). Permeability of premature eggs from Drosophila collected with the ‘ovitron’. Nature 206, 910913.
Zalokar, M. (1971). Transplantation of nuclei in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the U.S.A. 68, 15391541.
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Genetics Research
  • ISSN: 0016-6723
  • EISSN: 1469-5073
  • URL: /core/journals/genetics-research
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