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Genetic fine-structure of the GA-1 locus in the higher plant Arabidopsis thaliana (L.) Heynh

  • M. Koornneef (a1), J. Van Eden (a1), C. J. Hanhart (a1) and A. M. M. De Jongh (a1)

Summary

Non-germinating gibberellin (GA) responsive mutants are a powerful tool to study genetic fine structure in higher plants. Nine alleles (EMS-and fast neutron-induced) of the ga-1 locus of Arabidopsis thaliana were tested in a complete half-diallel. No wild type ‘recombinants’ were found in the selfed progeny of 9 homoallelic combinations (in total 3 × 105 plants); in the progenies from the 36 selfed hetero allelics the wild type frequency ranged from zero to 6·6 × 10−4. These frequencies allowed the construction of an internally consistent map for five different sites representing eight alleles. The ninth allele covered three sites and thus behaved like an intragenic deletion. The estimate of the total genetic length of the ga-1 locus was 0·07 cM. The order of the sites was also clearly reflected by the association with proximal outside markers. On the assumption that wild type gametes predominantly arise from reciprocal events, it was shown that a cross-over within the ga-1 locus leads to positive interference in the adjacent region.

The results are discussed with respect to the mutagen used, the frequencies found in other plant and Drosophila genes, and the possible occurrence of gene conversion.

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References

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Amano, E. (1968). Comparison of ethylmethanesulphonate and radiation-induced waxy mutants in maize. Mutation Research 5, 4146.
Amano, E. & Smith, H. H. (1965). Mutations induced by ethylmethanesulphonate in maize. Mutation Research 2, 344351.
Carlson, E. A. (1959). Comparative genetics of complex loci. The Quarterley Review of Biology 34, 3367.
Carlson, P. S. (1971). A genetic analysis of the rudimentary locus of Drosophila melanogaster. Genetical Research 17, 5381.
Catcheside, D. G. (1977). The Genetics of Recombination, pp. 172. London: Edward Arnold.
Chovnick, A., Ballantyne, G. H. & Holm, D. G. (1971). Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics 69, 179209.
Feenstra, W. J. (1965). An emasculation technique. Arabidopsis Information Service 2, 34.
Fincham, J. R. S., Day, P. R. & Radford, A. (1979). Fungal Genetics, pp. 197216. Oxford, London, Edinburgh, Melbourne: Blackwell.
Freeling, M. (1976). Intragenic recombination in maize: pollen analysis methods and the effect of parental Adh1 + isoalleles. Genetics 83, 707717.
Freeling, M. (1978). Allelic variation at the level of intragenic recombination. Genetics 89, 211224.
Grace, D. (1980). Genetic analysis of the dumpy complex locus in Drosophila melanogaster: complementation, fine structure and function. Genetics 94, 647662.
Green, M. M. & Green, K. C. (1956). A cytogenetic analysis of the lozenge pseudoalleles in Drosophila. Zeitschrift für indukt. Abstammungs- und Vererbungslehre 87, 708721.
Hilliker, A. J. & Chovnick, A. (1981). Further observations on intragenic recombination in Drosophila melanogaster. Genetical Research 38, 281296.
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genetical Research 5, 282304.
Jöroensen, J. H. & Jensen, H. P. (1979). Interallelic recombination in the ml-o locus in barley. Barley Genetics Newsletter 9, 3739.
Koornneef, M. (1979). Intragenic recombination within the ga-1 locus of Arabidopsis thaliana. Arabidopsis Information Service 16, 4146.
Koornneef, M., De Bruine, J. H. & Goetssch, P. (1980). A provisional map of chromosome 4 of Arabidopsis. Arabidopsis Information Service 17, 1118.
Koornneef, M., Dellaert, L. W. M. & Van Dek Veen, J. H. (1982 a). EMS-and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutation Research 93, 109123.
Koornneef, M. & Van Der Veen, J. H. (1980). Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) Heynh. Theoretical & Applied Genetics 58, 257263.
Koornneef, M., Van Der Veen, J. H., Spruit, C. J. P. & Karssen, C. M. (1981). Isolation and use of mutants with an altered germination behaviour in Arabidopsis thaliana and tomato. In Induced Mutations: a Tool in Plant Research (Vienna, IAEA), 227232.
Nelson, O. E. (1958). Intracistron recombination in the Wx/wx region of maize. Science 130, 794795.
Nelson, O. E. (1962). The waxy locus in maize. I. Intralocus recombination frequency estimates by pollen and by conventional analyses. Genetics 47, 737742.
Nelson, O. E. (1968). The waxy locus in maize II. The location of the controlling element alleles. Genetics 60, 507524.
Nelson, O. E. (1975). The waxy locus in maize. III. Effect of structural heterozygosity on intragenic recombination and flanking marker assortment. Genetics 79, 3144.
Nilan, R. A., Kleinhofs, A. & Warner, R. L. (1981). Use of induced mutants of genes controlling nitrate reductase, starch deposition, and anthocyanin synthesis in barley. In Induced Mutations: a Tool in Plant Research (Vienna, IAEA), pp. 183200.
Oostindier-Braaksma, F. J. & Feenstra, W. J. (1973). Isolation and characterization of chlorate-resistant mutants in Arabidopsis thaliana. Mutation Research 9, 165185.
Rosichan, J., Nilan, R. A., Arenaz, P. & Kleinhofs, A. (1979). Intragenic recombination at the waxy locus in Hordeum vulgare. Barley Genetics Newsletter 9, 7985.
Salamini, F. & Lorenzoni, C. (1970). Genetical analysis of glossy mutants of Maize. III. Intracistron recombination and high negative interference. Molecular and General Genetics 108, 225232.
Wettstein-Knowles, P. Van & Søgaard, B. (1980). The cer-cqu region in barley: gene cluster or multifunctional gene. Carlsberg Research Communications 45, 125141.
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Genetics Research
  • ISSN: 0016-6723
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