Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-23T09:10:14.302Z Has data issue: false hasContentIssue false
  • English
  • Français

A genetic study of female sterility in Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Orest M. Mylyk  and
S. F. H. Threlkeld
Orest M. Mylyk
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario, CanadaL8S 4K1
S. F. H. Threlkeld
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario, CanadaL8S 4K1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A genetic analysis following the initial detection of a female sterile variant resulted in the finding of seven or eight different female sterile mutants, most or all of which are on linkage groups I and II. They were present in heterokaryotic condition in already existing strains, except one which originated spontaneously during the study. All mutants fail to produce functional protoperithecia. Most of them, however, are able to function as female parents in heterokaryons. All mutants differ morphologically from the wild type, most being subtly different, but two being appreciably different. The apparently high frequency of occurrence of female sterile mutants suggests that protoperithecial development is under an elaborate genetic control. Differences in vegetative morphology appear to be a common property of mutants affecting the early stages of the sexual development.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 1 , August 1974 , pp. 91 - 102
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Apirion, D. (1966). Recessive mutants at unlinked loci which complement in diploids but not in heterokaryons of Aspergillus nidulans. Genetics 53, 935–941.CrossRefGoogle Scholar
Barbesgaard, P. O. & Wagner, S. (1959). Further studies on the biochemical basis of protoperithecia formation in Neurospora crassa. Hereditas 45, 564–572.CrossRefGoogle Scholar
Bhattacharya, L. & Feldman, J. F. (1971). A rapid screening procedure for female-sterile mutants. Neurospora Newsletter 18, 11.Google Scholar
Brockman, H. E. & De Serres, F. J. (1963). ‘Sorbose toxicity’ in Neurospora. American Journal of Botany 50, 709–714.CrossRefGoogle Scholar
Davis, R. H. & De Serres, F. J. (1970). Genetic and microbiological research techniques for Neurospora crassa. In: Methods in Enzymology Vol. 17 (ed. Tabor, H. and Tabor, C. W.), pp. 79–143. New York: Academic Press.Google Scholar
Dodge, B. O. (1946). Self-sterility in ‘bisexual’ heterokaryons of Neurospora. Bulletin of the Torrey Botanical Club 73, 410–416.CrossRefGoogle Scholar
Fitzgerald, P. H. (1963). Genetic and epigenetic factors controlling female sterility in Neurospora crassa. Heredity 18, 47–62.CrossRefGoogle ScholarPubMed
Ho, C. C. (1972). Mutations blocking development of the protoperithecium in Neurospora. Neurospora Newsletter 19, 15–16.Google Scholar
Horowitz, N. H., Fling, M., Macleod, H. L. & Sueoka, N. (1960). Genetic determination and enzymatic induction of tyrosinase in Neurospora. Journal of Molecular Biology 2, 96–104.CrossRefGoogle Scholar
Mitchell, M. B. (1966). Round spore character in N. crassa. Neurospora Newsletter 10, 6.Google Scholar
Mitchell, M. B., Mitchell, H. K. & Tissières, A. (1953). Mendelian and non-Mendelian factors affecting the cytochrome system in Neurospora crassa. Proceedings of the National Academy of Sciences, U.S.A. 39, 606–613.CrossRefGoogle ScholarPubMed
Mylyk, O. M. & Threlkeld, S. F. H. (1972). The use of heterokaryons in facilitating crosses between Neurospora strains having reduced female fetility. Neurospora Newsletter 19, 21.Google Scholar
Newmeyer, D. (1970). A suppressor of the heterokaryon-incompatibility associated with mating type in Neurospora crassa. Canadian Journal of Genetics and Cytology 12, 914–926.CrossRefGoogle ScholarPubMed
Newmeyer, D. & Taylor, C. W. (1967). A pericentric inversion in Neurospora, with unstable duplication progeny. Genetics 56, 771–791.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1972). An insertional translocation in Neurospora that generates duplications heterozygous for mating type. Genetics 71, 25–51.CrossRefGoogle ScholarPubMed
Perkins, D. D., Newmeyer, D., Taylor, C. W. & Bennett, D. C. (1969). New markers and map sequences in Neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40, 247–278.CrossRefGoogle ScholarPubMed
Pincheira, G. & Srb, A. M. (1969). Cytology and genetics of two abnormal ascus mutants of Neurospora. Canadian Journal of Genetics and Cytology 11, 281–286.CrossRefGoogle ScholarPubMed
Pontecorvo, G. (1963). Microbial genetics: retrospect and prospect. Proceedings of the Royal Society, B 158, 1–23.Google Scholar
Srb, A. M. (1957). Exotic growth forms in Neurospora. Journal of Heredity 48, 146–153.Google Scholar
Srb, A. M. & Basl, M. (1969 a). The isolation of mutants affecting ascus development in Neurospora crassa and their analysis by a zygote complementation test. Genetical Research 13, 303–311.CrossRefGoogle ScholarPubMed
Srb, A. M. & Basl, M. (1969 b). Abnormal ascus mutants. Neurospora Newsletter 15, 22.Google Scholar
Tan, S. T. & Ho, C. C. (1970). A gene controlling the early development of protoperithecium in Neurospora crassa. Molecular and General Genetics 107, 158–161.Google Scholar
Terenzi, H. F. & Reissig, J. L. (1967). Modifiers of the cot gene in Neurospora: the gulliver mutants. Genetics 56, 321–329.CrossRefGoogle ScholarPubMed
Threlkeld, S. F. H. (1962). Some asci with nonidentical sister spores from a cross in Neurospora crassa. Genetics 47, 1187–1198.CrossRefGoogle ScholarPubMed
Vigfusson, N. V. (1969). Sterility mutants in Neurospora crassa. (Abstract). Canadian Journal of Genetics and Cytology 11, 483.Google Scholar
Weijer, J. & Vigfusson, N. V. (1972). Sexuality in Neurospora crassa. I. Mutations to male sterility. Genetical Research 19, 191–204.CrossRefGoogle ScholarPubMed
Westergaard, M. & Hirsch, H. (1954). Environmental and genetic control of differentiation in Neurospora. Proceedings of the Symposium of the Colston Research Society 7, 171–183.Google Scholar
Westergaard, M. & Mitchell, H. K. (1947). Neurospora. V. A synthetic medium favouring sexual reproduction. American Journal of Botany 34, 573–577.CrossRefGoogle Scholar