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A mutant sex factor of Pseudomonas aeruginosa

  • V. A. Stanisich (a1) and B. W. Holloway (a1)

Summary

A mutant with the properties of a recipient has been isolated from the P. aeruginosa donor strain PAT (FP 2 + ) following treatment with the acridine-mustard ICR-191. While this strain displays the properties expected of a female or recipient in a number of genetic tests, the FP 2 determined property of mercury resistance is retained by the strain, suggesting that it may carry the FP2 factor in a mutated form. Treatment of the donor strain PAT (FP2 + ) with acridine-mustard has produced mutant male strains with the ability to form recombinants with other male strains at frequencies similar to that obtained in FP2+ × FP2 − matings. This characteristic has been shown to be due to a mutation in the FP2 factor which is dominant to the wild-type function. The isolation of stable male strains carrying both the mutant and wild type forms of the sex factor suggests that more than one copy of the FP 2 factor occurs in P. aeruginosa strain PAT donors.

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References

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Achtman, M., Willetts, N. S. & Clark, A. J. (1971). A genetic analysis oi conjugational transfer determined by the F factor in E. coli. I. Isolation and characterisation of mutants. Journal of Bacteriology 106, 529538.
Ames, B. N. & Whitfield, H. J. (1966). Frameshift mutagenesis in Salmonella. Cold Spring Harbor Symposium on Quantitative Biology 31, 221225.
Bazaral, M. & Helinski, D. R. (1968). Circular DNA forms of colicinogenic factors E1, E2 and E3 from E. coli. Journal of Molecular Biology 36, 185194.
Cozzarelli, N. R., Kelly, R. B. & Kornberg, A. (1968). A minute circular DNA from E. coli 15. Proceedings of the National Academy of Sciences, U.S.A. 60, 992999.
Dowman, J. E. & Meynell, G. G. (1970). Pleiotropic effects of de-repressed bacterial sex factors on colicinogeny and cell wall structure. Molecular and General Genetics 109, 5768.
Echols, H. (1963). Properties of F′ strains of E. coli superinfected with F-lac and F-gal episomes. Journal of Bacteriology 85, 262268.
Fargie, B. & Holloway, B. W. (1965). Absence of clustering of functionally related genes in Pseudomonas aeruginosa. Genetical Research, Cambridge 6, 284299.
Finnegan, D. J. & Willetts, N. S. (1971). Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Molecular and General Genetics 111, 256264.
Freifelder, D. R. & Freifelder, D. (1968). Studies on E. coli sex factors. I. Specific labelling of F'lac DNA. Journal of Molecular Biology 32, 1523.
Hickson, F. T., Roth, T. F. & Helenski, D. R. (1967). Circular DNA forms of a bacterial sex factor. Proceedings of the National Academy of Sciences, U.S.A. 58, 17311738.
Hirota, Y. (1960). The effect of acridine dyes on mating type factors of E. coli. Proceedings of the National Academy of Sciences, U.S.A. 46, 5764.
Holloway, B. W. (1955). Genetic recombination in Pseudomonas aeruginosa. Journal of General Microbiology 13, 572581.
Holloway, B. W. (1956). Self-fertility in Pseudomonas aeruginosa. Journal of General Microbiology 15, 221224.
Holloway, B. W. & Fargie, B. (1960). Fertility factors and genetic linkage in Pseudomonas aeruginosa. Journal of Bacteriology 80, 362368.
Holloway, B. W. & Jennings, P. A. (1958). An infectious fertility factor for Pseudomonas aeruginosa. Nature 181, 855856.
Holloway, B. W., Krishnapillai, V. & Stanisich, V. A. (1971). Pseudomonas genetics. Annual Review of Genetics (in the Press).
Jacob, F., Brenner, S. & Cuzin, F. (1963). On the regulation of DNA replication in bacteria. Cold Spring Harbor Symposium on Quantitative Biology 28, 329348.
Lederberg, J., Cavalli, L. L. & Lederberg, E. M. (1952). Sex compatibility in E. coli. Genetics 37, 720730.
Loutit, J. S. (1969). Investigation of the mating system of Pseudomonas aeruginosa strain 1. IV. Mapping of distal markers. Genetical Research, Cambridge 13, 9198.
Loutit, J. S. (1969). Investigation of the mating system of Pseudomonas aeruginosa strain 1. V. The effect of N-methyl-N′-nitro-N-nitrosoguanidine on a donor strain. Genetical Research, Cambridge 14, 103109.
Loutit, J. S. (1971). Investigation of the mating system of Pseudomonas aeruginosa strain 1. VI. Mercury resistance associated with the sex factor (FP). Genetical Research, Cambridge 16, 179184.
Loutit, J. S., Pearce, L. E. & Marinus, M. G. (1968 a). Investigation of the mating system of Pseudomonas aeruginosa strain 1. I. Kinetic studies. Genetical Research, Cambridge 12, 2936.
Loutit, J. S. & Marinus, M. G. (1968 b). Investigation of the mating system oi Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genetical Research, Cambridge 12, 3744.
Loutit, J. S., Marinus, M. G. & Pearce, L. E. (1968 c). Investigation of the mating system of Pseudomonas aeruginosa strain 1. III. Kinetic studies on the transfer of sex factor (FP). Genetical Research, Cambridge 12, 139145.
Novick, R. P. (1969). Extrachromosomal inheritance in bacteria. Bacteriological Reviews 33, 210263.
Ohtsubo, E. (1970). Transfer-defective mutants of sex factors in E. coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer. Genetics 64, 189197.
Ohtsubo, E., Nishimura, Y. & Hirota, Y. (1970). Transfer-deficient mutants of sex factors in E. coli. I. Defective mutants and complementation analysis. Genetics 64, 173188.
Richmond, M. H. (1970). Plasmids and chromosomes in prokaryotic cells, from Organisation and Control in Prokaryotic and Eukaryotic Cells. XIIth Symposium of the Society of General Microbiology. Cambridge University Press.
Rolfe, B. & Holloway, B. W. (1966). Alterations in host specificity of bacterial deoxyribonucleic acid after an increase in growth temperature of Pseudomonas aeruginosa. Journal of Bacteriology 92, 4348.
Rownd, R., Nakaya, R. & Nakamura, A. (1966). Molecular nature of the drug resistance factors of the Enterobacteriaceae. Journal of Molecular Biology 17, 376393.
Scaife, J. & Gross, J. D. (1962). Inhibition of multiplication of an F-lac factor in Hfr cells of Escherichia coli K12. Biochemical and Biophysical Research Communications 7, 403407.
Stanisich, V. A. (1968). Studies on the conjugation system of Pseudomonas aeruginosa. M.Sc. thesis, University of Melbourne.
Stanisich, V. A. & Holloway, B. W. (1969 a). Conjugation in Pseudomonas aeruginosa. Genetics 61, 327339.
Stanisich, V. A. & Holloway, B. W. (1969 b). Genetic effects of acridines on Pseudomonas aeruginosa. Genetical Research, Cambridge 13, 5770.
Takahashi, I. & Barnard, R. A. (1967). Effect of N-methyl-N′-nitro-nitrosoguanidine on the F factor of E. coli. Mutation Research 4, 111117.
Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of E. coli: partial purification and some properties. Journal of Biological Chemistry 218, 97106.
Waltho, J. A. & Holloway, B. W. (1966). Suppression of fluorophenylalanine resistance by mutation to streptomycin resistance in Pseudomonas aeruginosa. Journal of Bacteriology 92, 3542.
Willetts, N. S. (1967). The elimination of F-lac + from E. coli by mutagenic agents. Biochemical and Biophysical Research Communications 27, 112117.
Willetts, N. S. (1971). Plasmid specificity of two proteins required for conjugation in E. coli K12. Nature 203, 183185.

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