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  • Infection Control & Hospital Epidemiology, Volume 32, Issue 5
  • May 2011, pp. 507-509

Evaluation of ATP Bioluminescence Assays for Potential Use in a Hospital Setting

  • Zoie A. Aiken (a1), Michael Wilson (a1) and Jonathan Pratten (a1)
  • DOI:
  • Published online: 01 January 2015

ATP bioluminescence is being applied in hospitals to measure surface contamination. We compared commercial luminometers for detecting the number Staphylococcus aureus associated with surfaces. The data showed that the ATP bioluminescence methods tested were not robust enough to generate quantitative data on bacterial numbers, especially at low concentrations.

Corresponding author
Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WCIX 8LD, United Kingdom, (
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5.T Lewis , C Griffith , M Gallo , M Weinbren . A modified ATP benchmark for evaluating the cleaning of some hospital environmental surfaces. J Hosp Infect 2008;69:156163.

6.A Lundin . Use of firefly luciferase in ATP-related assays of bio-mass, enzymes, and metabolites. Methods Enzymol 2000;305:346370.

8.JA Poulis , M de Pijper , DA Mossel , PP Dekkers . Assessment of cleaning and disinfection in the food industry with the rapid ATP-bioluminescence technique combined with the tissue fluid contamination test and a conventional microbiological method. Int J Food Microbiol 1993;20:109116.

9.SJ Dancer . How do we assess hospital cleaning? a proposal for microbiological standards for surface hygiene in hospitals. J Hosp Infect 2004;56:1015.

10.G Moore , C Griffith . A comparison of traditional and recendy developed methods for monitoring surface hygiene within the food industry: an industry trial. Int J Environ Health Res 2002;12:317.

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