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The Inanimate Environment of an Intensive Care Unit as a Potential Source of Nosocomial Bacteria Evidence for Long Survival of Acinetobacter calcoaceticus

  • Sandra I. Getchell-White (a1), Leigh G. Donowitz (a1) and Dieter H.M. Groschel


Environmental surface and personnel hand impression cultures were obtained during 13 sampling periods in the University of Virginia Pediatric Intensive Care Unit to document potential reservoirs of nosocomial pathogens. In 78 environmental cultures Staphylococcus aureus was found eight times and gram-negative bacilli ten times. The patient chart cover was the most commonly contaminated surface. Acinetobacter calcoaceticus was found in five of ten cultures positive for gram-negative bacilli. Thirty of 59 hand cultures were positive for S aureus and gram-negative bacilli; nurses and residents had both, respiratory therapists only gram-negative bacilli, and A calcoaceticus was the most commonly isolated bacterium of potentially nosocomial significance (14/30). Laboratory investigation of bacterial survival revealed that gramnegative bacilli survived on a dry formica surface from a few hours up to three days but Acinetobacter survived up to 13 days. Since A calcoaceticus has been implicated in many nosocomial infections, its long survival on a dry surface may be an additional factor in its transmission in hospitals and suggests that more attention be paid to environmental surfaces as a source of significant nosocomial pathogens.


Corresponding author

Department of Pathology, Box 168, University of Virginia School of Medicine, Charlottesville, VA 22908


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1.Lennette, EH, Balows, A, Hausler, WJ, et al: Manual of Clinical Micro-&o&y, ed 4. Washington, DC, American Society of Microbiology, 1985.
2.Faller, A, Schleiffer, KH, et al: Modified oxidase and benzidine tests for separation of staphylococci from micrococci. J Clin Microbiol 1981; 13:10311035.
3.Wenzel, RP, Osterman, CA, Hunting, KJ, et al: Hospital acquired infections, I. Surveillance in a university hospital. Am f Epidemiol 1976; 103:251260.
4.Birnboim, HC, Doly, J: A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7: 15131523.
5.Maniatis, T, Fritsch, EF, Sambrook, J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, 1982.
6.National Committee for Clinical Laboratory Standards: Performance standards fov antimicrobial susceptibility testing. First Information Supplement, NCCLS document M100-S. Villanova, PA, 1986.
7.Joklik, WK, Willett, HP, Amos, DB: Zinsser's Microbiology, ed 18. Norwalk, Conn, Appleton-Century-Crofts, 1984, p 598.
8.Gill, FA, Gill, VJ: Acinetobacter species, in Mandell, GL, Douglas, RG, Bennett, JE (eds): Principles and Practice of Infectious Diseases, ed 2. New York, John Wiley & Sons, 1985, pp 12541256.
9.Allen, KD, Green, HT: Hospital outbreak of multi-resistant Acinetobacter anitratus: An airborne mode of spread.] Hosp Infect 1987; 9:110119.
10.Harstein, Al, Rashad, AL, Liebler, JM, et al: Multiple intensive care unit outbreak of Acinetobacter calcoaceticus subspecies anitratus respiratory infection and colonization associated with contaminated reusable ventilator circuits and resuscitation bags. Am J Med 1988; 85:624631.
11.Taplin, D, Rebell, G, Zaias, N: The human skin as a source of Mima-Herellea infections. JAMA 1963; 186:952953.
12.Fujita, K, Lilly, HA, Ayliffe, GAJ: Spread of resistant gram-negative bacilli in burn units./ Hosp Infect 1982; 3:2937.
13.Ayliffe, GAJ, Babb, JR, Davies, JG, et al: Hand disinfection: A comparison of various agents in laboratory and ward studies. J Hosp Infect 1988; 11:226243.
14.Buxton, AE, Anderson, RL, Werdegar, D, et al: Nosocomial respiratory tract infection and colonization with Acinetobacter calcoaceticus. Am J Med 1978; 65:507513.
15.Donowitz, LG: Handwashing technique in a pediatric intensive care unit. Am J Dis Child 1987; 141:683685.
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Infection Control & Hospital Epidemiology
  • ISSN: 0899-823X
  • EISSN: 1559-6834
  • URL: /core/journals/infection-control-and-hospital-epidemiology
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