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Monitoring Rotavirus Environmental Contamination in a Pediatric Unit Using Polymerase Chain Reaction

  • Hervé Soule (a1), Odile Genoulaz (a1), Bénédicte Gratacap-Cavallier (a1), Marie Reine Mallaret (a2), Patrice Morand (a1), Patrice François (a3), Dominique Luu Duc Bin (a2), Agnés Charvier (a4), Cécile Bost-Bru (a3) and Jean-Marie Seigneurin (a1)...


Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.


Corresponding author

Laboratoire de Virologie, CHU Grenoble, BP217, 38043 Grenoble Cedex 9, France


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