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Outbreak of Infection With Acinetobacter Strain RUH 1139 in an Intensive Care Unit

Published online by Cambridge University Press:  21 June 2016

Elsa Zuleima Salazar De Vegas ,
Beatriz Nieves ,
Maria Araque ,
Elsa Velasco ,
Joaquim Ruíz  and
Jordi Vila
Elsa Zuleima Salazar De Vegas
Affiliation:
Laboratorio de Bacteriología “Dr. Roberto Gabaldón”, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela
Beatriz Nieves
Affiliation:
Laboratorio de Bacteriología “Dr. Roberto Gabaldón”, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela
Maria Araque
Affiliation:
Laboratorio de Bacteriología “Dr. Roberto Gabaldón”, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela
Elsa Velasco
Affiliation:
Laboratorio de Bacteriología “Dr. Roberto Gabaldón”, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela
Joaquim Ruíz
Affiliation:
Centro de Salud Internacional, IDIBAPS, Hospital Clínic, Villarroel, Barcelona, Spain
Jordi Vila*
Affiliation:
Servei de Microbiología, IDIBAPS, Hospital Clinic, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
*
Servei de Microbiologia, IDIBAPS, Facultat de Medicina, Hospital Clínic, Villarroel 170, 08036-Barcelona, Spain (jvila@ub.edu)
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Abstract

Objective.

To investigate a nosocomial outbreak of infection with Acinetobacter strain RUH 1139, in the unit of high neonatal risk at University Hospital of The Andes (Mérida, Venezuela).

Methods.

Twenty-eight Acinetobacter strains were detected by biochemical testing and further identified to the species level by examination of the gene encoding 16S ribosomal DNA, using restriction analysis and gene sequencing. The epidemiological relationship between the strains was established by means of repetitive extragenic palindromic polymerase chain reaction (REP-PCR) and pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibilities were determined by disk diffusion.

Results.

The spread of an epidemic strain of Acinetobacter RUH 1139 among 16 patients over a period of 3 months was demonstrated using antimicrobial susceptibility testing, PFGE, and REP-PCR. The epidemic strain was also isolated in 2 of the sampled parenteral nutrition solutions. All the patients involved in the infection outbreak had received parenteral solution. Moreover, strains of Acinetobacter RUH 1139 with another PFGE pattern and of A. baumannii were sporadically isolated before and during the outbreak.

Conclusion.

This is the first description of an outbreak of infection with this genospecies of Acinetobacter in which parenteral nutrition solution was potentially the infection source.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 4 , April 2006 , pp. 397 - 403
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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