Skip to main content
×
Home
    • Aa
    • Aa

Purification procedure sensitizes Bacillus endospores to free radicals from UVA radiation and photocatalysis

  • Vijay Krishna (a1), Jue Zhao (a2), Ben Koopman (a2) and Brij Moudgil (a1)
Abstract
Abstract

Many researchers are investigating the extreme resilience of bacterial endospores against chemical and physical inactivating agents. The presence of vegetative cells in spore suspensions can result in overly optimistic assessment of inactivating agents; therefore, various spore purification methods have been applied to separate spores from vegetative cells prior to testing. The present study was undertaken to evaluate the effect of two widely used spore purification methodologies on spore integrity and susceptibility to ultraviolet-A (UVA) radiation and free radicals generated from photocatalysts. Bacillus subtilis and Bacillus cereus spores were purified by procedures that involved heat shock alone or chemical washes, lysozyme treatment and heat shock (CLH). The purified spores were exposed to UVA radiation or free radicals generated by photocatalyst and susceptibility were evaluated in terms of survival ratio. The effect of purification procedure on the spore morphology was investigated with electron microscopy. The CLH purification process significantly damages spore coats and increases the susceptibility of Bacillus spores to UVA radiation and photocatalytic inactivation. It is therefore likely that the survival of CLH treated spores in extra-terrestrial environments would be less than that of the same spores purified by a less aggressive procedure.

Copyright
Corresponding author
e-mail: krishnv2@ccf.org
Footnotes
Hide All
*

Present Address: Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.

Present address: Public Works Department, City of Salem, Salem, Oregon 97303, USA.

Footnotes
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

International Journal of Astrobiology
  • ISSN: 1473-5504
  • EISSN: 1475-3006
  • URL: /core/journals/international-journal-of-astrobiology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 3
Total number of PDF views: 7 *
Loading metrics...

Abstract views

Total abstract views: 45 *
Loading metrics...

* Views captured on Cambridge Core between 3rd August 2017 - 22nd August 2017. This data will be updated every 24 hours.