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Effect of pH, rearing temperature and sunlight on infectivity of Malaysian isolate of nucleopolyhedrovirus to larvae of Spodoptera litura (Lepidoptera: Noctuidae)

  • A.S. Sajap (a1), M.A. Bakir (a1), H.A. Kadir (a2) and N.A. Samad (a3)

The effects of pH, sunlight and rearing temperature on infectivity of nucleopolyhedrovirus (SpltNPV) were investigated against larvae of Spodoptera litura (Fabricius). The infectivity of virus increased with increased larval rearing temperature. The LT50 values were significantly reduced from 24 to 6 days when rearing temperature was increased from 20 to 35 °C. The pH of the substrate significantly affected the infectivity of the virus. The LT50 values increased to about fourfold when the pH of suspension was raised from 7 to 11. Infectivity of the virus was more adversely affected in alkaline than in acidic suspension. Sunlight also deleteriously affected infectivity of the virus. The LT50 values increased with the increase in the period of sunlight exposure. A complete viral inactivation occurred after 12 h of direct sunlight exposure.

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A. Ali and S. Y. Young (1991) Influence of larval age and temperature on effectiveness of a nuclear polyhedrosis virus in soybean looper Pseudoplusia includens (Lepidoptera: Noctuidae) on soybean. Biological Control 1, 334338.

R. W. Behle , M. R. McGuire and P. Tamez-Guerra (2000) Effect of light energy on alkali-released virions from Anagrapha falcifera. Journal of Invertebrate Pathology 76, 120126.

D. Grzywacz , D. J. McKinley , K. A. Jones and G. Moawad (1997) Microbial contamination in Spodoptera littoralis nuclear polyhedrosis virus produced in insects in Egypt. Journal of Invertebrate Pathology 69, 151156.

C. M. Ignoffo (1966) Effects of temperature on mortality of Heliothis zea larvae exposed to sublethal doses of a nuclear-polyhedrosis virus. Journal of Invertebrate Pathology 8, 290292.

C. M. Ignoffo and C. Garcia (1996) Simulated sunlight UV sensitivity of experimental dust formulations of the nuclear polyhedrosis virus of Helicoverpa/Heliothis. Journal of Invertebrate Pathology 67, 192194.

R. P. Jaques (1970) Application of viruses to soil and foliage for control of the cabbage looper and imported cabbageworm. Journal of Invertebrate Pathology 15, 328340.

D. W. Johnson , D. B. Boucias , C. C. Barfield and G. E. Allen (1982) A temperature-dependent developmental model for a nucleopolyhedrovirus of the velvet green caterpillar, Anticarsia gemmatalis. Journal of Invertebrate Pathology 40, 292298.

S. T. Keating , J. C. Schultz and W. G. Yendol (1990) The effect of diet on gypsy moth (Lymantria dispar) larval midgut pH, and its relationship with larval susceptibility to a baculovirus. Journal of Invertebrate Pathology 56, 317326.

P. J. McLeod , W. C. Yearin and Y. S. Young (1977) Inactivation of Baculovirus heliothis by ultraviolet irradiation, dew, and temperature. Journal of Invertebrate Pathology 30, 237241.

M. D. Monobrullah and M. Nagata (2001) Optical brighteners as ultraviolet protectants and as enhancers in pathogenicity of Spodoptera litura (Fabricius) (Lep., Noctuidae) nucleopolyhedrovirus). Journal of Applied Entomology 125, 377382.

F. Moscardi and D. R. Sosa-Gomez (1992) Uses of viruses against soybean caterpillar in Brazil, pp. 98109. In Pest Management in Soybean (Edited by L. G. Copping , M. B. Green and R. T. Ness ). Elsevier Applied Science, London, UK.

D. W. Pritchett , S. Y. Young and W. C. Aaron (1982) Dissolution of Autographa californica nuclear polyhedrosis virus polyhedra by the digestive fluid of Trichoplusia ni (Lepidoptera: Noctuidae) larvae. Journal of Invertebrate Pathology 39, 354361.

H. C. T. Ribeiro and O. H. O. Pavan (1994) Effect of temperature on the development of baculoviruses. Journal of Applied Entomology 118, 316320.

G. F. Rohrmann (1992) Review: Baculovirus structural proteins. Journal of General Virology 73, 749761.

A. S. Sajap , J. R. Kotulai , H. A. Kadir and M. Y. Hussein (1999) Impact of prey infected by nuclear polyhedrosis virus on a predator, Sycanus leucomesus Walk. (Hemiptera: Reduviidae). Journal of Applied Entomology 123, 9397.

M. Shapiro and R. Argauer (1995) Effects of pH, temperature and ultraviolet radiation on the activity of an optical brightener as a viral enhancer for the gypsy moth (Lepidoptera: Lymantriidae) baculovirus. Journal of Economic Entomology 88, 16021606.

M. T. B. Silva and F. Moscardi (2002) Field efficacy of the nucleopolyhedrovirus of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae): Effect of formulations, water pH, volume and time of application, and type of spray nozzle. Neotropical Entomology 31, 7583.

W. A. Smirnoff (1972) Effect of sunlight on the nuclear-polyhedrosis virus of Neodiprion swainei with measurement of the solar energy received. Journal of Invertebrate Pathology 19, 179188.

N. V. Van Beek , P. R. Hughes and H. A. Wood (2000) Effects of incubation temperature on the dose-survival time relationship of Trichoplusia ni larvae infected with Autographa californica nucleopolyhedrovirus. Journal of Invertebrate Pathology 76, 185190.

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International Journal of Tropical Insect Science
  • ISSN: 1742-7584
  • EISSN: 1742-7592
  • URL: /core/journals/international-journal-of-tropical-insect-science
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