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Genotype differences in photosynthetic characteristics and nitrogen efficiency of new-type oilseed rape responding to low nitrogen stress

Published online by Cambridge University Press:  30 July 2014

G. L. WANG ,
G. D. DING ,
F. S. XU ,
H. M. CAI ,
J. ZOU  and
X. S. YE
G. L. WANG
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
G. D. DING
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
F. S. XU
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
H. M. CAI
Affiliation:
Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
J. ZOU
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
X. S. YE*
Affiliation:
Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
*
*To whom all correspondence should be addressed. Email: xiangshengye@mail.hzau.edu.cn
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Summary

New-type oilseed rape (Brassica napus, ArArCcCc) with introgressed exotic subgenomic components from Brassica rapa (ArAr) and Brassica carinata (BcBcCcCc) showed strong heterosis in both vegetative and reproductive growth. The aim of the current study was to analyse the tolerance of the new-type B. napus with different exotic subgenomic contents to low nitrogen (N) stress. Under hydroponic culture and pot experiments, root system parameters, photosynthetic parameters, relative chlorophyll concentration (SPAD values), biomass, seed yield, seed yield components, N concentration and expressions of genes involved in N transport and assimilation were determined with two new-type B. napus genotypes (N-efficient genotype D4-15 and N-inefficient genotype D1-1) under high-N and low-N levels. Furthermore, N accumulation, N transfer efficiency and N use efficiency (NUE) were analysed in the two genotypes. The hydroponic and potted growth tests showed consistent characteristics in N uptake and utilization efficiency at the seedling stage, and N-efficient genotype (D4-15) showed better growth phenotypes across cultured conditions and N levels. Under the low-N condition, D4-15 produced a larger root system and accumulated more N, and had higher N transfer efficiency and NUE than D1-1. Moreover, D4-15 had significantly higher photosynthetic parameters, photosynthetic NUE and expression levels of the N transporter genes, BnNRT1·1, BnNRT2·5, BnNRT2·7 and BnAMT1·1, in roots or leaves, as well as higher seed yield than that of D1-1 under low-N supply. These results indicated that the N-efficient new-type B. napus D4-15 possessed excellent adaptability to low-N stress, which may be attributed to the highly introgressed exotic subgenomic components from B. rapa and B. carinata, suggesting the possibility of identifying high-nutrient-efficiency germplasm from inter-specific hybrids.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 153 , Issue 6 , August 2015 , pp. 1030 - 1043
Copyright
Copyright © Cambridge University Press 2014 

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