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Enhancing the DHA content in milk from dairy cows by feeding ALL-G-RICH™

  • C. A. Moran (a1), M. Morlacchini (a2) and G. Fusconi (a2)
Summary

The objective of this study was to evaluate the effect of the dietary inclusion of 6 g/kg dry matter intake of an unextracted Aurantiochytrium limacinum algae (AURA) in mid-lactation Italian Friesian cows under commercial conditions on milk yield, milk composition and docosahexaenoic acid (DHA) content. Cows were allocated to two groups (n = 18; 108.2 ± 66.1 and 104.4 ± 54.6 days in milk, control and treated groups, respectively). Feeding AURA for 84 d had no effect on dry matter intake, body condition score or weight gain, but did improve milk yield by 1.9 kg/cow/d (+5.4%; P < 0.1) over the course of the experiment. Milk fat concentration declined by 12% (P < 0.0001) without any significant change in 4% fat corrected milk, protein or lactose. Supplementing AURA for 12 weeks substantially altered the fatty acid profile of milk compared with milk from CON-fed cows such that the proportion of unsaturated fatty acids increased, omega-3 fatty acid content increased by 73.1% (P < 0.0001) and was accompanied by a favourable increase in the omega-3:6 fatty acid ratio by 75.0% (P < 0.0001). The AURA supplement, during day 7–84, increased the DHA concentration to 0.37 g /100 g milk total fatty acids (P < 0.0001) with a mean transfer efficiency of 18.1% from feed to milk. Together these results indicated that supplementing a dairy cow diet with DHA-rich microalgae is a feasible and efficient means for creating DHA-enriched milk for human consumption.

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Copyright
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
* Corresponding author: cmoran@alltech.com
References
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AbuGhazaleh, A. and Jenkins, T. (2004). Short Communication: Docosahexaenoic acid promotes vaccenic acid accumulation in mixed ruminal cultures when incubated with linoleic acid. Journal of Dairy Science 87: 10471050.
AbuGhazaleh, A., Potu, R. and Ibrahim, S. (2009) Short communication: The effect of substituting fish oil in dairy cow diets with docosahexaenoic acid-micro algae on milk compostion and fatty acid profile. Journal of Dairy Science 92: 61566159.
Amaru, D. and Field, C. (2009) Conjugated linoleic acid decreases mcf-7 human breast cancer cell growth and insulin-like growth factor-1 receptor levels. Lipids 44: 449458.
Angulo, J., Mahecha, L., Nuernberg, K., Nuernberg, G., Dannenberger, D., Olivera, M., Boutinaud, M., Leroux, C., Albrecht, E. and Bernard, L. (2012) Effects of polyunsaturated fatty acids from plant oils and algae on milk fat yield and composition are associated with mammary lipogenic and SREBF1 gene expression. Animal 6: 19611972.
Azrad, M., Turgeon, C. and Demark-Wahnefried, W. (2013) Current evidence linking polyunsaturated fatty acids with cancer risk and progression. Frontiers in Oncology 3: 112.
Bannon, C., Craske, J., Hiliker, A. (1985) Analysis of fatty acid methyl esters with high accuracy and reliability. Fats with fatty acids containing four or more carbon atoms. Journal of the American Oil Chemists Society 62: 15011507.
Bauman, D. and Griinari, J. (2003) Nutritional regulation of milk fat synthesis. Annual Review of Nutrition 23: 203227.
Boeckaert, C., Vlaeminch, B., Fievez, V., Maignien, L., Dijkstra, J. and Boon, N. (2008a) Accumulation of trans C18:1 fatty acids in the rumen after dietary algal supplementation is associated with changes in the Butyrivibrio community. Applied and Environmental Microbiology 74: 69236930.
Boeckaert, C., Vlaeminck, B., JDijkstra, J., Issa-Zacharia, A., Van Nespen, T., Can Straalen, W. and Fievez, V. (2008b) Effect of dietary starch or micro algae supplementation on rumen fermentation and milk fatty acid composition of dairy cows. Journal of Dairy Science 91: 47144727.
Bourre, J. (2005) Where to find omega-3 fatty acids and how feeding animals with diet enriched in omega-3 fatty acids to increase nutritional value of derived products for human: what is actually useful? The Journal of Nutrition Health and Aging 9: 232242.
Cheng, C., Ou, B., Shen, T. and Ding, S. (2006) Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in lay Tsaiya ducks. Asian-Australasian Journal of Animal Science 19: 10471053.
Chilliard, Y., Feray, A. and Doreau, M. (2001) Effect of different types of forages, animal fat or marine oils in cow's diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livestock Production Science 70: 3148.
Chow, T., Fievez, V., Moloney, A., Raes, K., Demeyer, D. and De Smet, S. (2004) Effect of fish oil on in vitro rumen lipolysis, apparent biohydrogenation of linoleic and linolenic acids and accumulation of biohydrogenation intermediates. Animal Feed Science and Technology 117: 112.
Connor, W. and Neuringer, M. (1988) The effects of n-3 fatty acid deficiency and repletion upon the fatty acid composition and function of the brain and retina. Progress in Clincal and Biological Research 282294, 275-.
Das, U. (2006a) Biological significance of essential fatty acids. Journal of the Association of Physicians of India 54: 309319.
Das, U. (2006b) Essential fatty acids - a review. Current Pharmaceutical Biotechnology 7: 467482.
Das, U. (2008) Can essential fatty acids reduce the burden of disease(s)? Lipids in Health and Disease 7: 15.
Donnelly, C., Olsen, A., Lewis, L., Eisenberg, B., Easstman, A. and Kinlaw, W. (2009) Conjugated linoleic acid (CLA) inhibits expression of the Spot 14 (THRSP) and fatty acid synthase genes and impairs the groth of human breast cancer and liposarcoma cells. Nutrition and Cancer 61: 114122.
EFSA. (2010) Scientific Opinion on Dietary Reference Values for fats, including saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, trans fatty acids, and cholesterol. EFSA Journal 8: 1461.
European Parliament and the Council of the European Union. (2010) Directive 2010/63/EU on the protection of animals used for scientific purposes. Official Journal of the European Union 276: 3379.
Folch, J., Lees, M., Sloane Stanley, G. (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226: 497509.
Fraeye, I., Bruneel, C., Lemahieu, C., Buyse, J., Muylaert, K. and Foubert, I. (2012) Dietary enrichment of eggs with omega-3 fatty acids: A review. Food research International 48: 961969.
Franklin, S., Martin, K., Baer, R., Schingoethe, D. and Hippen, A. (1999) Dietary marine algae (Schizochytrium sp.) increases concentrations of conjugated linoleic, docosahexaenoic and transvaccenic acids in milk of dairy cows. The Journal of Nutrition 129: 20482054.
Gallo, A., Moschini, M., Cerioli, C., Masoero, F. (2013) Use of principal component analysis to classify forages and predict their calculated energy content. Animal 7: 930939.
Glasser, F., Doreau, M., Ferlay, A. and Chilliard, Y. (2007) Technical note: Estimation of milk fatty acid yield from milk fat data. Journal of Dairy Science 90: 23022304.
Glover, K., Budge, S., Rose, M., Rupasinghe, H., MacLaren, L., Green-Johnson II, J. and Fredeen, A. (2012) Effect of feeding fresh forage and marine algae on the fatty acid composition and oxidation of milk and butter. Journal of Dairy Science 95: 27972809.
Griinari, J., Corl, B., Lacy, S., Chouinard, P., Nurmela, K. and Bauman, D. (2000) Conjugated linoleic acid is synthesized endogenouslyin lactating dairy cows by Δ9-desaturase. Journal of Nutrition 130: 22852291.
Hu, F., van Dam, R. and Liu, S. (2001) Diet and risk of Type II diabetes: The role of types of fat and carbohydrate. Diabetologia 44: 805817.
Jenkins, T., Wallace, R., Moate, P. and Mosley, E. (2008) Board-invited review: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Science 86: 397412.
Kelley, N., Hubbard, N. and Erickson, K. (2007) Conjugated linoleic acid isomers and cancer. Journal of Nutrition 137: 25992607.
Lien, E. (2009) Toxicology and safety of DHA. Prostaglandins, leukotrienes and essential fatty acids 81: 125132.
Lock, A. and Bauman, D. (2004) Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids 39: 11971206.
Loor, J. and Herbein, J. (2001) Alterations in blood plasma and milk fatty acid profiles of lactating Holstein cows in response to ruminal infusion of a conjugated linoleic acid mixture. Animal Research 51: 119134.
Lourenco, M., Ramos-Morales, E. and Wallace, R. (2010) The role of microbes in rumen lipolysis and biohydrogenation and their manipulation. Animal 4: 10081023.
Meadus, W., Duff, P., Uttaro, B., Aalhus, J., Rolland, D., Gibson, L. and Dugan, M. (2010) Production of docosahexaenoic acid (DHA) enriched bacon. Journal of Agricultural Food Chemistry 58: 465472.
Mente, A., de Koning, L., Shannon, H. and Anand, S. (2009) A systematic review of the evidence supporting a causal link between dietary factors and coronary heart disease. Archives of Internal Medicine 169: 659669.
Moate, P., Williams, S., Hannah, M., Eckard, R., Auldist, M., Ribaux, B., Jacobs, J. and Wales, W. (2013) Effects of feeding algal meal high in docosahexaenoic acid on feed intake, milk production, and methane emissions in dairy cows. Journal of Dairy Science 96: 31773188.
Moran, C. A., Fusconi, G., Morlacchini, M. and Jacques, K. A. (2017a) Docosahexaenoic acid content in longissimus dorsi and backfat tissues of grow-finish pigs fed diets containing 0, 0.25 or 0.5% heterotrophically grown algae: Study 2. Journal of Animal Science 95 (Suppl 2): 5859.
Moran, C. A., Fusconi, G., Morlacchini, M. and Jacques, K. A. (2017b) Changes in docosahexaenoic acid (DHA) content in longissimus dorsi and backfat tissue of finishing pigs given diets containing 1% heterotrophically grown algae during the last 28 days. Journal of Animal Science 95 (Suppl 2): 59.
Mosley, E., Shafii, B., Moate, P. and McGuire, M. (2006) Cis-9, trans-11 conjugated linoleic acid is synthesized directly from vaccenic acid in lactating dairy cattle. Journal of Nutrition 136: 570575.
Neal, B., Sacks, G., Swinburn, B., Vandevijvere, S., Dunford, E., Snowden, W., Webster, J., Barquera, S., Friel, S., Hawkes, C., Kelly, B., Kumanyika, S., L'abbe, M., Lee, A., Lobstein, T., Ma, J., Macmullan, J., Mohan, S., Monteiro, C., Rayner, M., Sanders, D., Walker, C. and INFORMAS (2013) Monitoring the levels of important nutrients in the food supply. Obesity Reviews, 4958.
Ney, D. (1991) Potential for enhancing the nutritional properties of milk fat. Journal of Dairy Science 74: 40024012.
Or-Rashid, M., GKramer, J., Wood, M. and McBride, B. (2008) Supplemental algae meal alters the ruminal trans-18:1 fatty acid and conjugated linoleic acid composition of cattle. Journal of Animal Science 86: 187196.
Palmquist, D. (2009) Omega-3 fatty acids in metabolism, health and nutrition and for modified animal product foods. Professional Animal Scientist 25: 207249.
Papadopoulos, G., Goulas, C., Apolstolaki, E. and Abril, R. (2002) Effects of dietary supplements of algae, containing polyunsaturated fatty acids, on milk yield and the composition of milk products in dairy ewes. Journal of Dairy Research 69: 357365.
Papanikolaou, Y., Brooks, J., Reider, C., Fulgoni, VL. (2014) U.S. adults are not meeting recommended levels for fish and omega-3 fatty acid intake: results of an analysis using observational data from NHANES 2003–2008. Nutrition Journal, 13: 31.
Park, P. and Goins, G. (1994) In situ preparation of fatty acid methyl esters for analysis of fatty acid composition in foods. Journal of Food Science 59: 12621266.
Raper, N., Cronin, F. and Exler, J. (1992) Omega-3 fatty acid content of the US food supply. Journal American College of Nutrition 11: 304308.
Reynolds, C., Cannon, V. and Loerch, S. (2006) Effects of forage source and supplementation with soybean and marine algae oil on milk fatty acid composition of ewes. Animal Feed Science and Technology 131: 333357.
Riserus, U., Willett, W. and Hu, F. (2009) Dietary fats and prevention of type 2 diabetes. Progress in Lipid Research 48: 4451.
Rymer, C. and Givens, D. (2005) n-3 fatty acid enrichment of edible tissue of poultry: A review. Lipids 40: 121130.
Santos, S., Oliveira, A. and Lopes, C. (2013) Systematic review of saturated fatty acids on inflammation and circulating levels of adipokines. Nutrition Research 33: 687695.
Sardi, L., Martelli, G., Lambertini, L., Parisini, P. and Mordenti, A. (2006) Effects of a dietary supplement of DHA-rich marine algae on Italian heavy pig production parameters. Livestock Science 103: 95103.
SAS Institute Inc. (2011) SAS/STAT User's Guide. Cary, NC, USA.
Sawilowsky, S. (2007) Mann-Whitney U test (Wilcoxon rank-sum test). In Encyclopedia of measurement and statistics (eds. Salkind, N and Rasmussen, K), pp. 565567, Sage Publications, Inc., Oakland, CA.
Schuchardt, J., Huss, M., Stauss-Grabo, M. and Hahn, A. (2010) Significance of long-chain polyunsaturated fatty acids (PUFAs) for the development and behaviour of children. European Journal of Pediatrics 169: 149164.
Shingfield, K., Bonnet, M. and Scollan, N. (2013) Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7: 132162.
Simopoulos, A., Leaf, A. and Salem, N. Jr. (1999) Essentiality of and recommended dietary intakes for omega-3 fatty acids. Annals of Nutrition and Metabolism 43: 127130.
Siri-Tarino, P., Sun, Q., Hu, F. and Krauss, (2010) Saturated fat, carbohydrate, and cardiovascular disease. American Journal of Clinical Nutrition 91: 502509.
Stamey, J., Shepherd, D., de Veth, M. and Corl, B. (2012) Use of algae or algal oil rich in n-3 fatty acids as a feed supplement for dairy cattle. Journal of Dairy Science 95: 52695275.
Steck, S., Chalecki, A., Miller, P., Conway, J., Austin, G., Hardin, J., Albright, C. and Thuillier, P. (2007) Conjugated linoleic acid supplementation for twelve weeks increases lean body mass in obese humans. Journal of Nutrition 137: 11881193.
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