Hostname: page-component-8448b6f56d-sxzjt Total loading time: 0 Render date: 2024-04-24T19:32:10.764Z Has data issue: false hasContentIssue false
  • English
  • Français

AGPAT3 Gene polymorphisms are associated with milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  15 July 2021

Xiaomei Sun ,
Yan Liang ,
Qisong Gao ,
Jiahe Guo ,
Cheng Tang ,
Kerong Shi ,
Zhangping Yang  and
Yongjiang Mao
Xiaomei Sun*
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Yan Liang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Qisong Gao
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Jiahe Guo
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Cheng Tang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Kerong Shi
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian271018, China
Zhangping Yang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou225009, China
Yongjiang Mao*
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou225009, China
*
Authors for correspondence: Xiaomei Sun, Email: sunxiaomei@live.com Yongjiang Mao, E-mail: cattle@yzu.edu.cn
Authors for correspondence: Xiaomei Sun, Email: sunxiaomei@live.com Yongjiang Mao, E-mail: cattle@yzu.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The current study reports the identification of previously undiscovered single-nucleotide polymorphisms (SNPs) in the bovine AGPAT3 gene and further investigates their associations with milk production traits. Our results demonstrate that the major allele C of the SNP g.12264 C > T is positively correlated with test-day milk yield, protein percentage and 305-day milk yield. Importantly, in silico analysis showed that the C/T transition at this locus gives rise to two new transcription factor binding sites (TFBS), E2F1 and Nkx3-2. Polymorphism g.18658 G > A was the only SNP associated with milk urea nitrogen (MUN) with the G allele related to an increase in milk urea nitrogen as well as fat percentage. The GG genotype of SNP g.28731 A > G was associated with the highest fat and protein percentage and lowest 305-day milk yield and somatic cell score (SCS). The association between AGPAT3 locus and milk production traits could be utilized in marker-assisted selection for the genetic improvement of milk production traits and, probably in conjunction with other traits, for selection to improve fitness of dairy cattle.

Keywords

1-acylglycerol-3-phosphate O-acyltransferase 3AssociationDairy cattleMilk production traitsSingle nucleotide polymorphisms
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 247 - 252
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Biao, L, Jiang, YJ, Yaling, Z and Hatch, GM (2005) Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARα in murine heart. Biochemical Journal 385, 469–477.Google Scholar
Bobbo, T, Tiezzi, F, Penasa, M, De Marchi, M and Cassandro, M (2018) Short communication: association analysis of diacylglycerol acyltransferase (DGAT1) mutation on chromosome 14 for milk yield and composition traits, somatic cell score, and coagulation properties in Holstein bulls. Journal of Dairy Science 101, 8087–8091.CrossRefGoogle ScholarPubMed
Bouwman, AC, Bovenhuis, H, Visker, MH and van Arendonk, JA (2011) Genome-wide association of milk fatty acids in Dutch dairy cattle. BMC Genetics 12, 43.CrossRefGoogle ScholarPubMed
Carmel, L and Chorev, M (2012) The function of introns. Frontiers in Genetics 3, 55.Google Scholar
Denechaud, P-D, Lopez-Mejia, IC, Giralt, A, Lai, Q, Blanchet, E, Delacuisine, B, Nicolay, BN, Dyson, NJ, Bonner, C and Pattou, F (2016) E2f1 mediates sustained lipogenesis and contributes to hepatic steatosis. The Journal of Clinical Investigation 126, 137–150.CrossRefGoogle ScholarPubMed
Dimova, DK and Dyson, NJ (2005) The e2f transcriptional network: old acquaintances with new faces. Oncogene 24, 2810–2826.CrossRefGoogle ScholarPubMed
Egger-Danner, C, Cole, J, Pryce, J, Gengler, N, Heringstad, B, Bradley, A and Stock, KF (2015) Invited review: overview of new traits and phenotyping strategies in dairy cattle with a focus on functional traits. Animal: An International Journal of Animal Bioscience 9, 191–207.CrossRefGoogle ScholarPubMed
Eicher, R, Bouchard, E and Bigras-Poulin, M (1999) Factors affecting milk urea nitrogen and protein concentrations in Quebec dairy cows. Preventive Veterinary Medicine 39, 53–63.CrossRefGoogle ScholarPubMed
Falconer, D and Mackay, T (1996) Introduction to Quantitative Genetics, 4th Edn, Harlow, UK: Longmans.Google Scholar
Fornes, O, Castro-Mondragon, JA, Khan, A, van der Lee, R, Zhang, X, Richmond, PA, Modi, BP, Correard, S, Gheorghe, M and Baranašić, D (2020) Jaspar 2020: update of the open-access database of transcription factor binding profiles. Nucleic Acids Research 48(D1), D87–D92.Google ScholarPubMed
Gabriel, S, Ziaugra, L and Tabbaa, D (2009) SNP genotyping using the sequenom massarray iplex platform. Current Protocols in Human Genetics 60, 2–12CrossRefGoogle Scholar
Jonker, J, Kohn, R and Erdman, R (1998) Using milk urea nitrogen to predict nitrogen excretion and utilization efficiency in lactating dairy cows. Journal of Dairy Science 81, 2681–2692.CrossRefGoogle ScholarPubMed
Lemaitre, RN, Tanaka, T, Tang, W, Manichaikul, A, Foy, M, Kabagambe, EK, Nettleton, JA, King, IB, Weng, L-C and Bhattacharya, S (2011) Genetic loci associated with plasma phospholipid n-3 fatty acids: a meta-analysis of genome-wide association studies from the charge consortium. PLoS Genetics 7, e1002193.CrossRefGoogle ScholarPubMed
Li, C, Sun, D, Zhang, S, Wang, S, Wu, X, Zhang, Q, Liu, L, Li, Y and Qiao, L (2014) Genome wide association study identifies 20 novel promising genes associated with milk fatty acid traits in Chinese Holstein. PLoS ONE 9, e96186.CrossRefGoogle ScholarPubMed
Li, X, Buitenhuis, AJ, Lund, MS, Li, C, Sun, D, Zhang, Q, Poulsen, NA and Su, G (2015) Joint genome-wide association study for milk fatty acid traits in Chinese and Danish Holstein populations. Journal of Dairy Science 98, 8152–8163.CrossRefGoogle ScholarPubMed
Li, M, Lu, X, Gao, Q, Wang, M, Arbab, AAI, Sun, Y, Chen, Z, Zhang, H, Karrow, NA and Yang, Z (2019) A functional 3′ utr polymorphism of fads2 affects cow milk composition through modifying mir-744 binding. Animals 9, 1090.CrossRefGoogle ScholarPubMed
Li, M, Gao, Q, Wang, M, Liang, Y, Sun, Y, Chen, Z, Zhang, H, Karrow, NA, Yang, Z and Mao, Y (2020) Polymorphisms in fatty acid desaturase 2 gene are associated with milk production traits in Chinese Holstein cows. Animals 10, 671.CrossRefGoogle ScholarPubMed
Lu, B, Jiang, YJ, Man, MQ, Brown, B, Elias, PM and Feingold, KR (2005) Expression and regulation of 1-acyl-sn-glycerol-3-phosphate acyltransferases in the epidermis. Journal of Lipid Research 46, 2448–2457.CrossRefGoogle ScholarPubMed
Mann, NJ, O'Connell, SL, Baldwin, KM, Singh, I and Meyer, BJ (2010) Effects of seal oil and tuna-fish oil on platelet parameters and plasma lipid levels in healthy subjects. Lipids 45, 669–681.CrossRefGoogle ScholarPubMed
Miglior, F, Sewalem, A, Jamrozik, J, Lefebvre, D and Moore, R (2006) Analysis of milk urea nitrogen and lactose and their effect on longevity in Canadian dairy cattle. Journal of Dairy Science 89, 4886–4894.CrossRefGoogle ScholarPubMed
Miglior, F, Sewalem, A, Jamrozik, J, Bohmanova, J, Lefebvre, D and Moore, R (2007) Genetic analysis of milk urea nitrogen and lactose and their relationships with other production traits in Canadian Holstein cattle. Journal of Dairy Science 90, 2468–2479.CrossRefGoogle ScholarPubMed
Miglior, F, Fleming, A, Malchiodi, F, Brito, LF, Martin, P and Baes, CF (2017) A 100-year review: identification and genetic selection of economically important traits in dairy cattle. Journal of Dairy Science 100, 10251–10271.CrossRefGoogle ScholarPubMed
Naftelberg, S, Schor, IE, Ast, G and Kornblihtt, AR (2015) Regulation of alternative splicing through coupling with transcription and chromatin structure. Annual Review of Biochemistry 84, 165–198.CrossRefGoogle ScholarPubMed
Olsen, HG, Knutsen, TM, Kohler, A, Svendsen, M, Gidskehaug, L, Grove, H, Nome, T, Sodeland, M, Sundsaasen, KK, Kent, MP, Martens, H and Lien, S (2017) Genome-wide association mapping for milk fat composition and fine mapping of a qtl for de novo synthesis of milk fatty acids on bovine chromosome 13. Genetics. Selection and Evolution 49, 20.CrossRefGoogle Scholar
Ouellette, C, Cormier, H, Rudkowska, I, Guénard, F, Lemieux, S, Couture, P and Vohl, M-C (2013) Polymorphisms in genes involved in the triglyceride synthesis pathway and marine omega-3 polyunsaturated fatty acid supplementation modulate plasma triglyceride levels. Lifestyle Genomics 6, 268–280.Google ScholarPubMed
Provot, S, Kempf, H, Murtaugh, LC, Chung, U-I, Kim, D-W, Chyung, J, Kronenberg, HM and Lassar, AB (2006) Nkx3. 2/bapx1 acts as a negative regulator of chondrocyte maturation. Development 133, 651–662.CrossRefGoogle ScholarPubMed
Shindou, H, Koso, H, Sasaki, J, Nakanishi, H, Sagara, H, Nakagawa, KM, Takahashi, Y, Hishikawa, D, Iizuka-Hishikawa, Y and Tokumasu, F (2017) Docosahexaenoic acid preserves visual function by maintaining correct disc morphology in retinal photoreceptor cells. Journal of Biological Chemistry 292, 12054–12064.CrossRefGoogle ScholarPubMed
Viale, E, Tiezzi, F, Maretto, F, De Marchi, M, Penasa, M and Cassandro, M (2017) Association of candidate gene polymorphisms with milk technological traits, yield, composition, and somatic cell score in Italian Holstein-Friesian sires. Journal of Dairy Science 100, 7271–7281.CrossRefGoogle ScholarPubMed
Wiggans, G and Shook, G (1987) A lactation measure of somatic cell count. Journal of Dairy Science 70, 2666–2672.CrossRefGoogle ScholarPubMed
Wilfling, F, Wang, H, Haas, JT, Krahmer, N, Gould, TJ, Uchida, A, Cheng, J-X, Graham, M, Christiano, R and Fröhlich, F (2013) Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the er to lipid droplets. Developmental Cell 24, 384–399.CrossRefGoogle ScholarPubMed
Yamashita, A, Hayashi, Y, Nemoto-Sasaki, Y, Ito, M, Oka, S, Tanikawa, T, Waku, K and Sugiura, T (2014) Acyltransferases and transacylases that determine the fatty acid composition of glycerolipids and the metabolism of bioactive lipid mediators in mammalian cells and model organisms. Progress in Lipid Research 53, 18–81.CrossRefGoogle Scholar
Zuk, O, Schaffner, SF, Samocha, K, Do, R, Hechter, E, Kathiresan, S, Daly, MJ, Neale, BM, Sunyaev, SR and Lander, ES (2014) Searching for missing heritability: designing rare variant association studies. Proceedings of the National Academy of Sciences 111, E455–E464.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Sun et al. supplementary material

Tables S1-S2

Download Sun et al. supplementary material(PDF)
PDF 162.4 KB