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Antimicrobial susceptibility and biofilm forming ability of staphylococci from subclinical buffalo mastitis

Published online by Cambridge University Press:  13 March 2023

Ece Koldaş Ürer ,
Özkan Aslantaş ,
Erhan Tek ,
Mehmet Ali Yılmaz  and
Yaşar Ergün
Ece Koldaş Ürer
Affiliation:
Department of Obstetrics and Gynecology, Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, 31060, Hatay, Turkey
Özkan Aslantaş*
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060, Hatay, Turkey
Erhan Tek
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060, Hatay, Turkey
Mehmet Ali Yılmaz
Affiliation:
International Center for Livestock Research and Training, Lalahan, Ankara, Turkey
Yaşar Ergün
Affiliation:
Department of Obstetrics and Gynecology, Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, 31060, Hatay, Turkey
*
Author for correspondence: Özkan Aslantaş, Email: ozkanaslantas@yahoo.com/aslantas@mku.edu.tr
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Abstract

The starting objective of this research communication was to determine the prevalence of subclinical mastitis in buffalo in Turkey. We also seeked to isolate and identify staphylococci, determine their antimicrobial susceptibilities and biofilm-forming abilities as well as investigating the presence of biofilm-related genes and microbial surface components recognizing adhesive matrix molecules. A total of 107 (66.9%) staphylococci (28 S. aureus and 79 coagulase-negative staphylococci, CoNS) were isolated from 160 mastitic milk samples collected from 200 lactating water buffalos. The staphylococci were especially resistant to beta-lactams except for cefoxitin but were less resistant to the other antimicrobials that were tested. Based on the Congo red agar method, 92.9% of the S. aureus and 70.9% of the CoNS isolates were positive for biofilm-forming ability, while all S. aureus and 97.5% of CoNS isolates were positive by a microtiter plate analysis. The presence of icaA and icaD genes was not always correlated with biofilm synthesis, and even in the absence of these genes, the isolates were able to synthesize biofilm.

Keywords

Antibiotic resistancebiofilmbuffalomastitisstaphylococci
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 1 , February 2023 , pp. 38 - 41
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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