Skip to main content
    • Aa
    • Aa

Effect of grazing fresh legumes or feeding silage on fatty acids and enzymes involved in the synthesis of milk fat in dairy cows

  • Lars Wiking (a1), Peter K Theil (a2), Jacob H Nielsen (a1) and Martin T Sørensen (a2)

The impact of fresh legume types or silage on the composition of milk fatty acids and transcription of enzymes involved in the synthesis of milk fat in cows was studied. Three groups of cows grazed high proportions of white clover, red clover and lucerne, respectively. A fourth group of cows was fed maize/grass silage. The cows grazing high proportions of legumes produced significantly more 18:1 trans-11, 18:2 cis9-trans11, 18:2 trans10-cis12 and 18:3 fatty acids than cows fed silage. White clover and lucerne grazing resulted in significantly lower output of 18:1 trans9 in milk than red clover grazing and maize/grass silages. Transcription of stearoyl-CoA desaturase (SCD) in mammary tissue was significantly increased by grazing high proportions of legume whereas fatty acid synthase and acetyl-CoA carboxylase were not affected by type of feeding. Furthermore, average milk fat globule diameter was correlated to daily milk fat yield but was not affected by feeding. Although the fresh forage affected the transcription of SCD in mammary tissue, the largest effects were on the trans11-based fatty acids. It is concluded that type of forage, i.e. fresh or silage, had a greater impact on rumen fermentation pattern than on transcription of enzymes involved in the synthesis of milk fat.

Corresponding author
*For correspondence; e-mail:
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

LH Baumgard , E Matitashvili , BA Corl , DA Dwyer & DE Bauman 2002 trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. Journal of Dairy Science 85 21552163

L Bernard , J Rouel , C Leroux , A Ferlay , Y Faulconnier , P Legrand & Y Chilliard 2005 Mammary lipid metabolism and milk fatty acid secretion in Alpine goats fed vegetable lipids. Journal of Dairy Science 88 14781489

RW Brownsey , AN Boone , JE Elliot , JE Kulpa & WM Lee 2006 Regulation of acetyl-CoA carboxylase. Biochemical Society Transactions 34 223227

G Butler , JH Nielsen , T Slots , C Seal , MD Eyre , R Sanderson & C Leifert 2008 Fatty acids and fat-soluble antioxidant concentration in milk from high- and low-input conventional and organic systems: seasonal variation. Journal of the Science of Food and Agriculture 88 14301441

M Collomb , UR Bütikofer , B Sieber , B Jeangros & JO Bosset 2002 Correlation between fatty acids in cows' milk fat produced in lowlands, mountains and highland of Switzerland and botanical composition of the fodder. International Dairy Journal 12 661666

S Couvreur , C Hurtaud , PG Marnet , P Faverdin & JL Peyraud 2007 Composition of milk fat from cows selected for milk fat globule size and offered either fresh pasture or a corn silage-based diet. Journal of Dairy Science 90 392403

L Delbecchi , CE Ahnadi , JJ Kennelly & P Lacasse 2001 Milk fatty acid composition and mammary lipid metabolism in Holstein cows fed protected or unprotected canola seeds. Journal of Dairy Science 84 13751381

RJ Dewhurst , RT Evans , ND Scolland , JM Moorby , RJ Merry & RJ Wilkins 2003b Comparison of grass and legume silages for milk production. 1. Production response with different levels of concentrate. Journal of Dairy Science 86 26122621

PC Garnsworthy , LL Masson , AL Lock & TT Mottram 2006 Variation of milk citrate with stage of lactation and de novo fatty acid synthesis in dairy cows. Journal Dairy Science 89 16041612

RG Jensen 2002 The composition of bovine milk lipids: January 1995 to December 2000. Journal of Dairy Science 85 295350

J Knudsen 1972 Fatty acid synthetase from cow mammary gland tissue cells. Biochimica Biophysica Acta 280 408414

JV Nørgaard , LH Baumgard , TS Nielsen , PK Theil , MT Sørensen & K Sejersen 2008 Mammary cell turnover and expression of lipogenic genes in response to silage induces CLA and diet energy density in lactating cows. Acta Agriculturae Scandinavica A 58 113120

G Ortis-Gonzalez , R Jimenz-Flores , DR Bremmes , JH Clark , EJ DePeters , SJ Schimdt & JK Drackley 2007 Functional properties of butter oil made from bovine milk with experimentally altered fat composition. Journal of Dairy Science 90 50185031

CM Paton & JM Ntambi 2009 Biochemical and physiological function of stearoyl-CoA desaturase. American Journal of Physiology Endocrinology and Metabolism 297 E28–37

KA Poulsen , JF Young , P Theil , M Kolko , N Oksbjerg & N Lambert 2006 Role of phospholipase A2 in the induction of drip loss in pork. Journal of Agricultural and Food Chemistry 55 19701976

T Slots , G Butler , C Leifert , T Kristensen , LH Skibsted & JH Nielsen 2009 Potentials to differentiate milk composition by different feeding strategies. Journal of Dairy Science 92 20572066

MT Sorensen , JV Nørgaard , PK Theil , M Vestergaard & K Sejrsen 2006 Cell turnover and activity in mammary tissue during lactation and the dry period in dairy cows. Journal of Dairy Science 89 46324639

H Soyeurt , F Dehareng , P Mayeres , C Bertozzi & N Gengler 2008 Variation of Δ9-desaturase activity in dairy cattle. Journal of Dairy Science 91 32113224

H Timmen & S Patton 1988 Milk fat globules: Fatty acid composition, size and in vivo regulation of fat liquidity. Lipids 23 685689

PK Theil , IL Sørensen , M Therkildsen & N Oksbjerg 2006 Changes in proteolytic enzyme mRNAs relevant for meat quality during myogenesis of primary porcine satellite cell. Meat Science 73 335343

HA Van Dorland , M Kreuzer , H Leuenberger & H-R Wettstein 2008 Comparative potential of white and red clover to modify the milk fatty acid profile of cows fed ryegrass-based diets from zero-grazing and silage systems. Journal of the Science of Food and Agriculture 88 7585

CM Whiting , T Mutsvangwa , JP Walton , JP Cant & BW McBride 2004 Effects of feeding either fresh alfalfa or alfalfa silage on milk fatty acid content in Holstein dairy cows. Animal Feed Science and Technology 113 2737

L Wiking , L Björck & JH Nielsen 2003 The influence of feed on stability of fat globules during pumping of raw milk. International Dairy Journal 13 797803

L Wiking , J Stagsted , L Björck & JH Nielsen 2004 Milk fat globule size is affected by fat production in dairy cows. International Dairy Journal 14 909913

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *