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Dominance of the genus Polaromonas in the microbial ecology of an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating dairy processing wastewater under varying aeration rates

  • Beatriz Gil-Pulido (a1) (a2), Emma Tarpey (a3), William Finnegan (a3), Xinmin Zhan (a3), Alan DW Dobson (a1) (a2) and Niall O'Leary (a1) (a2)...
Abstract

In this Research Communication we investigate potential correlations between key bacterial groups and nutrient removal efficiency in an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating synthetic dairy processing wastewater. Reactor aeration rates of 0·6 and 0·4 litre per minute (LPM) were applied to an 8 l laboratory scale system and the relative impacts on IASBR microbial community structure and orthophosphate (PO4-P) and ammonium (NH4-N) removal efficiencies compared. Aeration at 0·6 LPM over several sludge retention times (SRTs) resulted in approximately 92% removal efficiencies for both PO4-P and NH4-N. Biomass samples subjected to next-generation sequencing (NGS), 16S rRNA profiling revealed a concomitant enrichment of Polaromonas under 0·6 LPM conditions, up to ~50% relative abundance within the reactor biomass. The subsequent shift in reactor aeration to 0·4 LPM, over a period of 3 SRTs, resulted in markedly reduced nutrient removal efficiencies for PO4-P (50%) and NH4-N (45%). An 85·7% reduction in the genus level relative abundance of Polaromonas was observed under 0·4 LPM aeration conditions over the same period.

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*For correspondence; e-mail: n.oleary@ucc.ie
References
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APHA 2005 Standard methods for the examination of water and waste water, 21st edn. American Public Health Association, Washington, DC
Bragg, L, Stone, G, Imelfort, M, Hugenholtz, P & Tyson, GW 2012 Fast, accurate error-correction of amplicon pyrosequences using Acacia. Nature Methods 9 425426
Caporaso, JG, Kuczynski, J, Stombaugh, J, Bittinger, K, Bushman, FD, Costello, EK, Fierer, N, Peña, AG, Goodrich, JK, Gordon, JI & Huttley, GA 2010 QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7 335336
Demirel, B, Yenigun, O & Onay, TT 2005 Anaerobic treatment of dairy wastewaters: a review. Process Biochemistry 40 25832595
Ferrera, I & Sánchez, O 2016. Insights into microbial diversity in wastewater treatment systems: how far have we come? Biotechnology Advances 34 790802
Ge, H, Batstone, DJ & Keller, J 2015 Biological phosphorus removal from abattoir wastewater at very short sludge ages mediated by novel PAO clade Comamonadaceae. Water Research, 69 173182
He, S, Ding, LL, Xu, K, Geng, JJ & Ren, HQ 2016 Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge. Bioresource Technology 211 494501
Pan, M, Henry, LG, Liu, R, Huang, X & Zhan, X 2013 Nitrogen removal from slaughterhouse wastewater through partial nitrification followed by denitrification in intermittently aerated sequencing batch reactors at 11 °C. Environmental Technology 35 470477
Weissbrodt, DG, Shani, N & Holliger, C 2014 Linking bacterial population dynamics and nutrient removal in the granular sludge biofilm ecosystem engineered for wastewater treatment. FEMS Microbiology Ecology 88 579595
Willems, A 2014 The family Comamonadaceae. In Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (eds) The Prokaryotes, pp. 777851. Springer Berlin, Heidelberg
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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
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