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Identification of 14 new single nucleotide polymorphisms in the bovine SLC27A1 gene and evaluation of their association with milk fat content

Published online by Cambridge University Press:  29 January 2008

Laura Ordovas ,
Pilar Zaragoza ,
Juan Altarriba  and
Clementina Rodellar
Laura Ordovas*
Affiliation:
Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, Spain
Pilar Zaragoza
Affiliation:
Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, Spain
Juan Altarriba
Affiliation:
Grupo de Mejora Genética, Facultad de Veterinaria, Universidad de Zaragoza, Spain
Clementina Rodellar
Affiliation:
Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, Spain
*
*For correspondence; e-mail: lordovas@unizar.es
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Abstract

The solute carrier family 27 member 1 (SLC27A1) is an integral membrane protein involved in the transport of long-chain fatty acids across the plasma membrane. This protein has been implicated in diet-induced obesity and is thought to be important in the control of energy homeostasis. In previous reports, our group described the isolation and characterization of the bovine SLC27A1 gene. The bovine gene is organized in 13 exons spanning over more than 40 kb of genomic DNA and maps in BTA 7 where several quantitative trait loci for fat related traits have been described. Because of its key role in lipid metabolism and its genomic localization, in the present work the search for variability in the bovine SLC27A1 gene was carried out with the aim of evaluating its potential association with milk fat content in dairy cattle. By sequencing analysis of all exons and flanking regions 14 new single nucleotide polymorphisms (SNPs) were identified: 1 in the promoter, 7 in introns and 6 in exons. Allele frequencies of all the SNPs were calculated by minisequencing analysis in two groups of Holstein-Friesian animals with highest and lowest milk-fat content estimated breeding values as well as in animals of two Spanish cattle breeds, Asturiana de los Valles and Menorquina. In the conditions assayed, no significant differences between Holstein-Friesian groups were found for any of the SNPs, suggesting that the SLC27A1 gene may have a poor or null effect on milk fat content. In Asturiana and Menorquina breeds all the positions were polymorphic with the exception of SNPs 1 and 8 in which C allele was fixed in both of them.

Keywords

SLC27A1 genemilk fatbovine
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 2 , May 2008 , pp. 129 - 134
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Aretz, S, Uhlhaas, S, Sun, Y, Pagenstecher, C, Mangold, E, Caspari, R, Moslein, G, Schulmann, K, Propping, P & Friedl, W 2004 Familial adenomatous polyposis: aberrant splicing due to missense or silent mutations in the APC gene. Human Mutation 24 370–380CrossRefGoogle ScholarPubMed
Berner, LA 1993 Defining the role of milkfat in balanced diets. Advances in Food and Nutrition Research 37 131–257CrossRefGoogle ScholarPubMed
Black, PN, Zhang, Q, Weimar, JD & DiRusso, CC 1997 Mutational analysis of a fatty acyl-coenzyme A synthetase signature motif identifies seven amino acid residues that modulate fatty acid substrate specificity. Journal of Biological Chemistry 272 4896–4903CrossRefGoogle ScholarPubMed
Buchanan, FC, Fitzsimmons, CJ, Van Kessel, AG, Thue, TD, Winkelman-Sim, DC & Schmutz, SM 2002 Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genetics, Selection, Evolution 34 105–116CrossRefGoogle ScholarPubMed
Capon, F, Allen, MH, Ameen, M, Burden, AD, Tillman, D, Barker, JN & Trembath, RC 2004 A synonymous SNP of the corneodesmosin gene leads to increased mRNA stability and demonstrates association with psoriasis across diverse ethnic groups. Human Molecular Genetics 13 2361–2368CrossRefGoogle ScholarPubMed
Casas, E, Shackelford, SD, Keele, JW, Koohmaraie, M, Smith, TP & Stone, RT 2003 Detection of quantitative trait loci for growth and carcass composition in cattle. Journal of Animal Science 81 2976–2983CrossRefGoogle ScholarPubMed
Chiu, HC, Kovacs, A, Blanton, RM, Han, X, Courtois, M, Weinheimer, CJ, Yamada, KA, Brunet, S, Xu, H, Nerbonne, JM, Welch, MJ, Fettig, NM, Sharp, TL, Sambandam, N, Olson, KM, Ory, DS & Schaffer, JE 2005 Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy. Circulation Research 96 225–233CrossRefGoogle ScholarPubMed
Gertow, K, Skoglund-Andersson, C, Eriksson, P, Boquist, S, Orth-Gomer, K, Schenck-Gustafsson, K, Hamsten, A & Fisher, RM 2003 A common polymorphism in the fatty acid transport protein-1 gene associated with elevated post-prandial lipaemia and alterations in LDL particle size distribution. Atherosclerosis 167 265–273CrossRefGoogle ScholarPubMed
Gimeno, RE 2007 Fatty acid transport proteins. Current Opinion in Lipidology 18 271–276CrossRefGoogle ScholarPubMed
Hui, TY, Frohnert, BI, Smith, AJ, Schaffer, JE & Bernlohr, DA 1998 Characterization of the murine fatty acid transport protein gene and its insulin response sequence. Journal of Biological Chemistry 273 27420–27429CrossRefGoogle ScholarPubMed
Kim, JK, Gimeno, RE, Higashimori, T, Kim, HJ, Choi, HJ, Punreddy, S, Mozell, RL, Tan, G, Stricker-Krongrad, A, Hirsch, DJ, Fillmore, JJ, Liu, ZX, Dong, JY, Cline, G, Stahl, A, Lodish, HF & Shulman, GI 2004 Inactivation of fatty acid transport protein 1 prevents fat-induced insulin resistance in skeletal muscle. Journal of Clinical Investigation 113 756–763CrossRefGoogle ScholarPubMed
Kimchi-Sarfaty, C, Oh, JM, Kim, IW, Sauna, ZE, Calcagno, AM, Ambudkar, SV & Gottesman, MM 2007 A “silent” polymorphism in the MDR1 gene changes substrate specificity. Science 315 525–528CrossRefGoogle Scholar
Komar, AA, Lesnik, T & Reiss, C 1999 Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation. FEBS Letters 462 387–391CrossRefGoogle ScholarPubMed
Lucey, JA, Johnson, ME & Horne, DS 2003 Invited review: Perspectives on the basis of the rheology and texture properties of cheese. Journal of Dairy Science 86 2725–2743CrossRefGoogle ScholarPubMed
Martin, G, Nemoto, M, Gelman, L, Geffroy, S, Najib, J, Fruchart, JC, Roevens, P, de Martinville, B, Deeb, S & Auwerx, J 2000 The human fatty acid transport protein-1 (SLC27A1; FATP-1) cDNA and gene: organization, chromosomal localization, and expression. Genomics 66 296–304CrossRefGoogle ScholarPubMed
Meirhaeghe, A, Martin, G, Nemoto, M, Deeb, S, Cottel, D, Auwerx, J, Amouyel, P & Helbecque, N 2000 Intronic polymorphism in the fatty acid transport protein 1 gene is associated with increased plasma triglyceride levels in a French population. Arteriosclerosis, Thrombosis and Vascular Biology 20 1330–1334CrossRefGoogle Scholar
Ordovas, L, Roy, R, Zaragoza, P, Hayes, H, Eggen, A & Rodellar, C 2005 Assignment of the solute carrier family 27 member 1 (SLC27A1) gene to bovine chromosome 7. Animal Genetics 36 352–353CrossRefGoogle ScholarPubMed
Ordovas, L, Roy, R, Zaragoza, P & Rodellar, C 2006 Structural and functional characterization of the bovine solute carrier family 27 member 1 (SLC27A1) gene. Cytogenetic and Genome Research 115 115–122CrossRefGoogle ScholarPubMed
Pastinen, T, Partanen, J & Syvanen, AC 1996 Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation. Clinical Chemistry 42 1391–1397CrossRefGoogle ScholarPubMed
Ron, M, Feldmesser, E, Golik, M, Tager-Cohen, I, Kliger, D, Reiss, V, Domochovsky, R, Alus, O, Seroussi, E, Ezra, E & Weller, JI 2004 A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. Journal of Dairy Science 87 476–490CrossRefGoogle ScholarPubMed
Roy, R, Ordovas, L, Zaragoza, P, Romero, A, Moreno, C, Altarriba, J & Rodellar, C 2006 Association of polymorphisms in the bovine FASN gene with milk-fat content. Animal Genetics 37 215–218CrossRefGoogle ScholarPubMed
Schaffer, JE & Lodish, HF 1994 Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell 79 427–436CrossRefGoogle ScholarPubMed
Stahl, A, Evans, JG, Pattel, S, Hirsch, D & Lodish, HF 2002 Insulin causes fatty acid transport protein translocation and enhanced fatty acid uptake in adipocytes. Developmental Cell 2 477–488CrossRefGoogle ScholarPubMed
Stuhlsatz-Krouper, SM, Bennett, NE & Schaffer, JE 1998 Substitution of alanine for serine 250 in the murine fatty acid transport protein inhibits long chain fatty acid transport. Journal of Biological Chemistry 273 28642–28650CrossRefGoogle ScholarPubMed
Stuhlsatz-Krouper, SM, Bennett, NE & Schaffer, JE 1999 Molecular aspects of fatty acid transport: mutations in the IYTSGTTGXPK motif impair fatty acid transport protein function. Prostaglandins, Leukotrienes and Essential Fatty Acids 60 285–289CrossRefGoogle ScholarPubMed
Van Laere, AS, Nguyen, M, Braunschweig, M, Nezer, C, Collette, C, Moreau, L, Archibald, AL, Haley, CS, Buys, N, Tally, M, Andersson, G, Georges, M & Andersson, L 2003 A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425 832–836CrossRefGoogle Scholar
Winter, A, Kramer, W, Werner, FA, Kollers, S, Kata, S, Durstewitz, G, Buitkamp, J, Womack, JE, Thaller, G & Fries, R 2002 Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences of the USA 99 9300–9305CrossRefGoogle Scholar
Wood, JD, Richardson, RI, Nute, GR, Fisher, AV, Campo, MM, Kasapidou, E, Sheard, PR & Enser, M 2004 Effects of fatty acids on meat quality: a review. Meat Science 66 21–32CrossRefGoogle ScholarPubMed
Wu, Q, Ortegon, AM, Tsang, B, Doege, H, Feingold, KR & Stahl, A 2006 FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity. Molecular and Cellular Biology 26 3455–3467CrossRefGoogle ScholarPubMed