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Molecular characterisation of the buffalo SCAP gene and its association with milk production traits in water buffaloes

Published online by Cambridge University Press:  22 May 2018

Tingxian Deng ,
Xiaoya Ma ,
Chunying Pang ,
Shasha Liang ,
Xingrong Lu ,
Anqin Duan  and
Xianwei Liang
Tingxian Deng
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xiaoya Ma
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Chunying Pang
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Shasha Liang
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xingrong Lu
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Anqin Duan
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xianwei Liang*
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
*
*For correspondence; e-mail: liangbri@126.com
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Abstract

The study reported in this Research Communication was conducted to investigate the molecular characterisation of buffalo SCAP gene, expression analysis, and the association between single nucleotide polymorphisms and milk production traits in 384 buffaloes. Sequence analysis revealed the SCAP gene had an open reading frame of 3837 bp encoding 1279 amino acids. A ubiquitous expression profile of SCAP gene was detected in various tissues with extreme predominance in the mammary gland during early lactation. Moreover, eleven SNPs in buffalo SCAP gene were identified, six of them (g.1717600A>G, g.1757922C>T, g.1758953G>A, g.1759142C>T, g.1760740G>A, and g.1766036T>C) were found to be significantly associated with 305-day milk yield. Thus, buffalo SCAP could sever as a candidate gene affecting milk production traits in buffalo and the identified SNPs might potentially be genetic markers.

Keywords

Buffalomilk production traitsSCAPsingle nucleotide polymorphism
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 133 - 137
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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