Skip to main content
    • Aa
    • Aa

Production of hydrogen peroxide by a small molecular mass compound in milk from Holstein cows with high and low milk somatic cell count

  • Senkiti Sakai (a1), Eriko Nonobe (a1), Takahiro Satow (a2), Kazuhiko Imakawa (a1) and Kentaro Nagaoka (a1)...

Mastitis is the most frequent and prevalent production disease in dairy herds in developed countries. Based on a milk somatic cell count (SCC) of either >300 000 or <200 000 cells/ml in this study, we defined the quarter as either inflamed or uninflamed, respectively. The electrical conductivity (EC) of milk was used as an indicator of udder epithelial cell damage. We determined the amount of H2O2 produced by utilizing a small molecular weight compound in milk, and examined the characteristics of H2O2 production and EC in milk from inflamed and uninflamed quarters. In cows with milk of delivery grade (control population), H2O2 production and EC were 3·6±1·3 nmol/ml and 5·4±0·4 mS/cm (mean±sd), respectively. In 37 inflamed quarter milk samples, the production of H2O2 was 1·9±1·0 nmol/ml and was significantly smaller than that in the control population (P<0·01). Production of H2O2 was moderately but significantly correlated with EC (r<−0·71). In 20 cows with inflamed quarters, the production of H2O2 in milk from inflamed quarters was significantly smaller than that in milk from uninflamed quarters (P<0·01). In 18 out of 20 cows, milk from inflamed quarters showed the smallest H2O2 production among all tested quarters in each cow. We conclude that inflammation caused a decrease in H2O2 production in milk. In this study, we present parameters for evaluating the lactoperoxidase/H2O2/thiocyanate antibacterial defence system in bovine milk.

Corresponding author
*For correspondence; e-mail:
Hide All
Bansal BK, Hamann J, Grabowski NT & Singh KB 2005 Variation in the composition of selected milk fraction samples from healthy and mastitic quarters, and its significance for mastitis diagnosis. Journal of Dairy Research 72 144152
Barett NE, Grabduson AS & Lewis MJ 1999 Contribution of the lactoperoxidase system to the keeping quality of pasteurized milk. Journal of Dairy Research 66 7380
Bisharat N, Crook DW, Leigh J, Harding RM, Ward PN, Coffey TJ, Maiden MC, Peto T & Jones PN 2004 Hyperinvasive neonatal group B streptococcus has arisen from a bovine ancestor. Journal of Clinical Microbiology 42 21612167
Björck L, Rosen CG, Marshall V & Reiter B 1975 Antibacterial activity of the lactoperoxidase system in milk against pseudomonads and other Gram-negative bacteria. Applied Microbiology 30 199204
Bradley AJ 2002 Bovine mastitis: an evolving disease. Veterinary Journal 164 116128
Bramley AJ & Dodd FH 1984 Reviews on the progress of dairy science: mastitis control—progress and prospects. Journal of Dairy Research 51 481512
De Oliveira AP, Watts JL, Salmon SA & Aarestrup FM 2000 Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and the United States. Journal of Dairy Science 83 855862
Ekstrand B 1989 Antimicrobial factors in milk—a review. Food Biotechnology 3 105126
Fonteh FA, Grandison AS & Lewis MJ 2002 Variations of lactoperoxidase activity and thiocyanate content in cows' and goats' milk throughout lactation. Journal of Dairy Research 69 401409
Furtmüller PG, Jantschko W, Regesberger G, Jakopitsch C, Arnhold J & Obinger C 2002 Reaction of lactoperoxidase compound I with halides and thiocyanate. Biochemistry 41 1189511900
Hillerton JE & Walton AW 1991 Identification for subclinical mastitis with a hand-held electrical conductivity meter. Veterinary Research 128 513515
Hogarth CJ, Fitzpatrick JL, Nolan AM, Young FJ, Pitt A & Eckersall PD 2004 Differential protein composition of bovine whey: a comparison of whey from healthy animals and from those with clinical mastitis. Proteomics 4 20942100
Jensen NE & Knudsen K 1991 Interquarter comparison of markers of subclinical mastitis: somatic cell count, electrical conductivity, N-acetyl-beta-glucosaminidase and antitrypsin. Journal of Dairy Research 58 389399
Kitchen BJ 1981 Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research 48 167188
Kitchen BJ, Middleton G, Durward IG, Andrews RJ & Salmon MC 1980 Mastitis diagnositic tests to estimate mammary gland epithelial cell damage. Journal of Dairy Science 63 978983
Korhonen HJ & Reiter B 1983 Production of H2O2 by bovine blood and milk polymorphonuclear leucocytes. Acta Microbiology Poland 32 5364
Milner P, Page KL, Walton AW & Hillerton JE 1996 Detection of clinical mastitis by changes in electrical conductivity of foremilk before visible changes in milk. Journal of Dairy Science 79 8386
Neave FK, Dodd FH & Kingwill RG 1966 A method of controlling udder disease. Veterinary Record 78 521523
Nielen M, Deluyker H, Schukken YH & Brand A 1992 Electrical conductivity of milk: measurements, modifiers, and meta analysis of mastitis detection performance. Journal of Dairy Science 75 606614
Pyörälä S 2003 Indicators of inflammation in the diagnosis of mastitis. Veterinary Research 34 565578
Rainard P & Riollet C 2006 Innate immunity of the bovine mammary gland. Veterinary Research 37 369400
Reiter B & Harnulv BG 1984 Lactoperoxidase antibacterial system: natural occurrence, biological functions and practical applications. Journal of Food Protection 47 724732
Sakai S, Satow T, Imakawa K & Nagaoka K 2008 Generation of hydrogen peroxide by a low molecular weight compound in whey of Holstein dairy cows. Journal of Dairy Research (in press)
Seegers H, Fourichon C & Beaudeau F 2003 Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34 475491
Shoshani E & Berman A 1998 Subclinical assessed by deviations in milk yield and electrical resistance. Journal of Dairy Research 65 3141
Sloth KHMN, Friggens NC, Løvendahl P, Andersen PH, Jensen J & Ingvatsen KL 2003 Potential for improving description of bovine udder health status by combined analysis of milk parameters. Journal of Dairy Science 86 12211232
White DG & McDermott PF 2001 Emergence and transfer of antibiotic resistance. Journal of Dairy Science 84 E151E155
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 1
Total number of PDF views: 10 *
Loading metrics...

Abstract views

Total abstract views: 62 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th October 2017. This data will be updated every 24 hours.