Skip to main content
×
×
Home

Proteolysis during storage of UHT milk: differences between whole and skim milk

  • Rosina López-Fandiño (a1), Agustin Olano (a1), Nieves Corzo (a1) and Mercedes Ramos (a1)
Summary
Summary

Proteolysis during storage of UHT skim and whole milks processed by either direct or indirect systems has been studied. All the proteolysis indices determined (measurement of free amino groups, PAGE of caseins and PAGE and reversed-phase HPLC of the fraction soluble at pH 4·6) revealed greater proteolytic degradation during storage of skim milks compared with that of whole milks subjected to the same UHT treatments. Increased activities of both native milk proteinase and proteinases of bacterial origin were observed in skim UHT milks. The different behaviour of UHT skim and whole milks on storage would have to be taken into account in establishing the process conditions.

Copyright
References
Hide All
Andrews A. T. 1983 Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 50 4555
Andrews A. T. & Alichanidis E. 1983 Proteolysis of caseins and the proteose-peptone fraction of bovine milk. Journal of Dairy Research 50 275290
Chianese L., Mauriello R., Moio L., Intorcia N. & Addeo F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 3947
Cousin M. A. 1982 Presence and activity of psychrotrophic microorganisms in milk and dairy products: a review. Journal of Food Protection 45 172207
De Koning P. J., Badings H. T., Van Der Pol J. J. G., Kaper J. & Vos-Klompmaker E. A. J. 1990 Effect of heat treatment and fat content on the properties of UHT milk. 23rd International Dairy Congress, Montreal, Canada. Brief Communications and Abstracts of Posters 2 807
Elgel W. N. 1977 Formation of γ1-A2, γ2-A2 and γ3-A caseins by in vitro proteolysis of β-casein A2 with bovine plasmin. International Journal of Biochemistry 8 187192
Fairbairn D. J. & Law B. A. 1986 Proteinases of psychrotrophic bacteria: their production, properties, effects and control. Journal of Dairy Research 53 139177
Grufferty M. B. & Fox P. F. 1988 Review article: milk alkaline proteinase. Journal of Dairy Research 55 609630
Harwalkar V. R. 1982 Age gelation of sterilized milks. In Developments in Dairy Chemistry—1 Proteins, pp. 229269 (Ed. Fox P. F.). London: Applied Science
Igarashi Y. 1990 Enhancement of proteolysis in bovine skim milk by heat and chemical treatments. Journal of Dairy Research 57 541548
Laemmli U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680685
López-Fandiño R., Olano A., San José C. & Ramos M. 1993 Application of reversed-phase HPLC to the study of proteolysis in UHT milk. Journal of Dairy Research 60 111116
McKellar R. C. 1981 Development of off-flavors in ultra-high temperature and pasteurized milk as a function of proteolysis. Journal of Dairy Science 64 21382145
Olano A., Calvo M. M. & Reglero G. 1986 Analysis of free carbohydrates in milk using micropacked columns. Chromatographia 21 538540
Parnell-Clunies E., Kakuda Y., Irvine D. & Mullen K. 1988 Heat-induced protein changes in milk processed by vat and continuous heating systems. Journal of Dairy Science 71 14721483
Resmini P., Pellegrino L., Hogenboom J. A. & Andreini R. 1989 Thermal denaturation of whey protein in pasteurized milk. Fast evaluation by HPLC. Italian Journal of Food Science 1 5162
Winter A., Ek K. & Andersson V. 1977 Analytical electrofocusing in thin layers of polyacrylamide gels. Bromma, Sweden: LKB-Produkter (LKB Application Note 250)
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 17 *
Loading metrics...

Abstract views

Total abstract views: 319 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 16th December 2017. This data will be updated every 24 hours.