Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 1
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Martínez-Augustin, Olga Rivero-Gutiérrez, Belén Mascaraque, Cristina and Sánchez de Medina, Fermín 2014. Food Derived Bioactive Peptides and Intestinal Barrier Function. International Journal of Molecular Sciences, Vol. 15, Issue. 12, p. 22857.


The influence of protein fractions from bovine colostrum digested in vivo and in vitro on human intestinal epithelial cell proliferation

  • Alison J Morgan (a1), Lisa G Riley (a1) (a2), Paul A Sheehy (a1) and Peter C Wynn (a1) (a3)
  • DOI:
  • Published online: 16 January 2014

Colostrum consists of a number of biologically active proteins and peptides that influence physiological function and development of a neonate. The present study investigated the biological activity of peptides released from first day bovine colostrum through in vitro and in vivo enzymatic digestion. This was assessed for proliferative activity using a human intestinal epithelial cell line, T84. Digestion of the protein fraction of bovine colostrum in vitro was conducted with the enzymes pepsin, chymosin and trypsin. Pepsin and chymosin digests yielded protein fractions with proliferative activity similar to that observed with undigested colostrum and the positive control foetal calf serum (FCS). In contrast trypsin digestion significantly (P<0·05) decreased colostral proliferative activity when co-cultured with cells when compared with undigested colostrum. The proliferative activity of undigested colostrum protein and abomasal whey protein digesta significantly increased (P<0·05) epithelial cell proliferation in comparison to a synthetic peptide mix. Bovine colostrum protein digested in vivo was collected from different regions of the gastrointestinal tract (GIT) in newborn calves fed either once (n=3 calves) or three times at 12-h intervals (n=3 calves). Digesta collected from the distal duodenum, jejunum and colon of calves fed once, significantly (P<0·05) stimulated cell proliferation in comparison with comparable samples collected from calves fed multiple times. These peptide enriched fractions are likely to yield candidate peptides with potential application for gastrointestinal repair in mammalian species.

Corresponding author
*For correspondence; e-mail:
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

HEAbud , NWatson & JKHeath 2005 Growth of intestinal epithelium in organ culture is dependent on EGF signalling. Experimental Cell Research 303 252262

AH Andrews 2004 Colostrum – not just for 24 h. Cattle Practise 12 121124

AJ Brosnahan & DR Brown 2012 Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations. Veterinary Microbiology 156 229–37

D Burleigh , K Fernandes & D Perrit 2000 T84 epithelial cells respond to 5-hydroxtryptamine when grown in serum-free media. European Journal of Pharmacology 390 103106

DEW Chatterton , G Smithers , P Roupas & A Brodkorb 2006 Bioactivity of beta -lactoglobulin and alpha -lactalbumin – technological implications for processing. International Dairy Journal 16 12291240

M Coste , V Rochet , D Leonil , S Molle , S Bouballab & D Tome 1992 Identification of C-terminal peptides of bovine B-casein that enhance proliferation of rat lymphocytes. Immunology Letters 33 4146

M Darewicz , B Dziuba , P Mickiewicz & J Dziuba 2011 The preventive potential of milk and colostrum proteins and protein fragments. Food Reviews International 27 357388

D Dee , J Pencer , S Nieh , J Krueger , J Katsaras & R Yada 2006 Comparison of solution structures and solubilities of native, partially unfolded and partially refolded pepsin. Biochemisty 45 1398213992

W Diehl-Jones & D Askin 2004 Nutritional modulation of neonatal outcomes. AACN Clinical Issues 15 8396

RP Donato , A El-Merhibi , B Gundsambuu , KY Mak , ER Formosa , X Wang , CA Abbott & BC Powell 2011 Studying permeability in a commonly used epithelial cell line: T84 intestinal epithelial cells. Methods in Molecular Biology 763 115137

A-M Fiat , D Migliore-Samour & P Jolles 1993 Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities. Journal of Dairy Science 76 301310

LS Ganjam , WHJ Thornton , RT Marshall & RS MacDonald 1997 Antiproliferative effects of yogurt fractions obtained by membrane dialysis on cultured mammalian intestinal cells. Journal of Dairy Science 80 23252329

NG Gregory 2003 Effect of enhancing curd formation during the first colostrum feed on absorption of & gamma; glutamyl transferase by newborn calves. Australian Veterinary Journal 81 549552

P Guilloteau , T Corring , R Toullec & J Robelin 1984 Enzyme potentialitites of the abomasum and pancreas of the calf. I. – Effect of age in the preruminant. Reproduction, Nutrition, Development 24 315325

M Jovani , B Viadel , Laparra Laparra , R Barber & R Farre 2004 Improvement of analytical conditions of mineral Caco-2 cell uptake assays. Food Science and Technology International 10 197201

M Klagsbrun & J Neumann 1979 The serum-free growth of Balb/c 3T3 cells in medium supplemented with bovine colostrum. Journal of Supramolecular Structure 11 349359

MKlagsbrun 1980 Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture. Journal of Cell Biology 84 808814

J Klimes , P Jagos , J Bounda & S Gajdusek 1986 Basic qualitative parameters of cow colostrum and their dependence on season and post partum time. Acta Veterinaria Brno 55 2339

H Korhonen , A Pihlanto-Leppala , P Rantamaki & T Tupasela 1998 Impact of processing on bioactive proteins and peptides. Trends in Food Science and Technology 9 307319

BA McCormick , SP Colgan , C Delp-Archer , SI Miller & JL Madara 1993 Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils. Journal of Cell Biology 123 895907

JF Medrano & L Sharrow 1989 Milk protein typing of bovine mammary gland tissue used to generate a complementary deoxyribonucleic acid library. Journal of Dairy Science 72 31903196

H Meisel & W Bockelmann 1999 Bioactive peptides encrypted in milk proteins: proteolytic activation and thropho-functional properties. Antonie Van Leeuwenhoek 76 207215

A Quaroni & J Holchman 1996 Development of intestinal cell culture models for drug transport and metabolism studies. Advanced Drug Delivery Reviews 22 352

OT Ramirez , GK Sureshkumar & R Mutharasan 1990 Bovine colostrum or milk as a serum substitute for the cultivation of a mouse hybridoma. Biotechnology and Bioengineering 35 882889

H Schagger & G Von Jagow 1987 Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kda. Analytical Biochemistry 166 368379

FL Schanbacher , RS Talhouk & FA Murray 1997 Biology and origin of bioactive peptides in milk. Livestock Production Science 50 105123

NP Shah 2000 Effects of milk-derived bioactives: an overview. British Journal of Nutrition 84 (Suppl 1.) S3S10

AK Singh , S Pandita , MM Vaidya , SV Singh , G Chandra , ZA Pampoori , R Huozha , MM Pathan , R Kushwaha & VK Sharma 2011 Bovine colostrum and neonate immunity – a review. Agricultural Reviews 32 7990

LG Sternhagen & JC Allen 2001 Growth rates of a human colon adenocarcinoma cell line are regulated by the milk protein alpha-lactalbumin. Advances in Experimental Medicine and Biology 501 115120

R Toullec , J-P Lalles , 2001 Digestion of colostrum by the preruminant calf: digestibility and origin of undigested protein fractions in ileal digesta. Lait 81 443454

M Yamada , K Murakami , 2002 Identification of low-abundance proteins of bovine colostral and mature milk using two-dimensional electrophoresis followed by microsequencing and mass spectrometry. Electrophoresis 23 11531160

M Yvon , I Van Hille , 1984 In vivo milk digestion in the calf abomasum. II. Milk and whey proteolysis. Reproduction, Nutrition, Development 24 835843

M Yvon , J-P Pelissier , 1985 In vivo milk digestion in the calf abomasum. III Amino acid compositions of the digesta leaving the abomasum. Reproduction, Nutrition, Development 25 495504

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *