Skip to main content
×
×
Home

Meconium proteins as a source of biomarkers for the assessment of the intrauterine environment of the fetus

  • B. Lisowska-Myjak (a1), E. Skarżyńska (a1) and M. Bakun (a2)
Abstract

Intrauterine environmental factors can be associated with perinatal complications and long-term health outcomes although the underlying mechanisms remain poorly defined. Meconium formed exclusively in utero and passed naturally by a neonate may contain proteins which characterise the intrauterine environment. The aim of the study was proteomic analysis of the composition of meconium proteins and their classification by biological function. Proteomic techniques combining isoelectrofocussing fractionation and LC-MS/MS analysis were used to study the protein composition of a meconium sample obtained by pooling 50 serial meconium portions from 10 healthy full-term neonates. The proteins were classified by function based on the literature search for each protein in the PubMed database. A total of 946 proteins were identified in the meconium, including 430 proteins represented by two or more peptides. When the proteins were classified by their biological function the following were identified: immunoglobulin fragments and enzymatic, neutrophil-derived, structural and fetal intestine-specific proteins. Meconium is a rich source of proteins deposited in the fetal intestine during its development in utero. A better understanding of their specific biological functions in the intrauterine environment may help to identify these proteins which may serve as biomarkers associated with specific clinical conditions/diseases with the possible impact on the fetal development and further health consequences in infants, older children and adults.

Copyright
Corresponding author
*Address for correspondence: Dr hab. Barbara Lisowska-Myjak, Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland. E-mail basia.myjak@interia.pl
References
Hide All
1. Skogen, JC, Øverland, S. The fetal origins of adult disease: a narrative review of the epidemiological literature. JRSM Short Rep. 2012; 3, 5966.
2. Miles, HL, Hofman, PL, Cutfield, WS. Fetal origins of adult disease: a paediatric perspective. Rev Endocr Disord. 2005; 6, 261268.
3. Olsen, J, David, B. (1938–2013) – a giant in reproductive epidemiology. Acta Obstet Gynecol Scand. 2014; 93, 10771080.
4. Ng, PC, Lam, HS. Biomarkers in neonatology: the next generation of tests. Neonatology. 2012; 102, 145151.
5. Ostrea, EM. Understanding drug testing in the neonate and the role of meconium analysis. J Perinat Neonatal Nurs. 2001; 14, 6182.
6. Lisowska-Myjak, B, Pachecka, J. Alpha-1-antitrypsin and IgA in serial meconium and faeces of healthy breast-fed newborns. Fetal Diagn Ther. 2007; 22, 116120.
7. Lisowska-Myjak, B, Pachecka, J. Trypsin and antitrypsin activities and protein concentration in serial meconium and feces of healthy newborns. J Matern Fetal Neonatal Med. 2006; 19, 477482.
8. Laforgia, N, Baldassarre, ME, Pontrelli, G, et al. Calprotectin levels in meconium. Acta Paediatr. 2003; 92, 463466.
9. Lisowska-Myjak, B, Żytyńska-Daniluk, J. Changes in physiology and pathophysiology of calprotectin excretion from neonate to adult. J Mol Biomark Diagn. 2015; 6, 15.
10. Wessel, D, Flügge, UI. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem. 1984; 138, 141143.
11. Mikula, M, Gaj, P, Dzwonek, K, et al. Comprehensive analysis of the palindromic motif TCTCGCGAGA: a regulatory element of the HNRNPK promoter. DNA Res. 2010; 17, 245260.
12. Brosch, M, Choudhary, J. Scoring and validation of tandem MS peptide identification methods. Methods Mol Biol. 2010; 604, 4353.
13. Käll, L, Canterbury, JD, Weston, J, Noble, WS, MacCoss, MJ. Semi-supervised learning for peptide identification from shotgun proteomics datasets. Nat Methods. 2007; 4, 923925.
14. Käll, L, Storey, JD, MacCoss, MJ, Noble, WS. Assigning significance to peptides identified by tandem mass spectrometry using decoy databases. J Proteome Res. 2008; 7, 2934.
15. Stewart, A, Malhotra, A. Gestational diabetes and the neonate: challenges and solutions. Res Rep Neonatol. 2015; 5, 3139.
16. Drozdowski, LA, Clandinin, T, Thomson, ABR. Ontogeny, growth and development of the small intestine : understanding pediatric gastroenterology. World J Gastroenterol. 2010; 16, 787799.
17. Neu, J. Gastrointestinal development and meeting the nutritional needs of premature infants. Am J Clin Nutr. 2007; 85, 629S634S.
18. Lee, J, Romero, R, Lee, KA, et al. Meconium aspiration syndrome: a role for fetal systemic inflammation. Am J Obstet Gynecol. 2016; 214 , 366.e1–9.
19. Kääpä, P, Soukka, H. Phospholipase A2 in meconium-induced lung injury. J Perinatol. 2008; 28(Suppl. 3), S120122.
20. De Luca, D, Minucci, A, Tripodi, D, et al. Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates. Intensive Care Med. 2011; 37, 11581165.
21. Lou, J, Mai, X, Lozoff, B, et al. Prenatal iron deficiency and auditory brainstem responses at 3 and 10 months: a pilot study. Hong Kong J Paediatr. 2016; 20, 7179.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Developmental Origins of Health and Disease
  • ISSN: 2040-1744
  • EISSN: 2040-1752
  • URL: /core/journals/journal-of-developmental-origins-of-health-and-disease
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Type Description Title
WORD
Supplementary materials

Lisowska-Myjak et al. supplementary material
Lisowska-Myjak et al. supplementary material 1

 Word (1.6 MB)
1.6 MB