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Larval migration of the ascarid nematode Toxocara canis following infection and re-infection in the gerbil Meriones unguiculatus

Published online by Cambridge University Press:  04 September 2015

M.C. Flecher ,
C. Musso ,
I.V.F. Martins  and
F.E.L. Pereira
M.C. Flecher*
Affiliation:
Veterinary Medicine Course and Medicine Course, Vila Velha University, Vila Velha, ES, Brazil
C. Musso
Affiliation:
Medicine Course, Vila Velha University, Vila Velha, ES, Brazil Infectious Diseases Unit, Federal University of Espirito Santo, Vitoria, ES, Brazil
I.V.F. Martins
Affiliation:
Veterinary Medicine Course, Federal University of Espirito Santo, Alegre, ES, Brazil
F.E.L. Pereira
Affiliation:
Medicine Course, Vila Velha University, Vila Velha, ES, Brazil Infectious Diseases Unit, Federal University of Espirito Santo, Vitoria, ES, Brazil
*
*E-mail: mayraflecher@yahoo.com.br
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Abstract

A morphological and immunohistochemical study of larval migration patterns was performed in gerbils that were infected once (primary infected group) or twice (secondary infected group) with 1500 eggs of Toxocara canis. Animals from the primary infected and the re-infected group were killed at different times after infection, and larvae were counted in the intestines, liver, lungs and brain. Fragments of all organs were formalin fixed and paraffin embedded for histology and immunohistochemistry analyses (using polyclonal anti-Toxocara serum raised in rabbits infected with T. canis). In the primary infected group, larvae were more abundant in the intestine at 24 h, in the liver and lungs between 24 and 72 h and in the brain after 96 h; larvae predominated in the brain for up to 60 days after infection. In the re-infected group, an increase in the number of larvae in the liver and a reduction in the number of larvae in the brain was observed up to 60 days after re-infection. Inflammatory reactions were absent or limited. Eosinophils and loose granulomata were observed around the larvae and their antigens in the primary infected group and were more severe. Many eosinophils and typical epithelioid granulomata were observed around larvae in the re-infected group. These results demonstrate that the migration pattern of T. canis larvae in gerbils is similar to that in mice and rats, exhibiting a late neurotropic stage. In the re-infected group, there was histological evidence of an adaptive T-helper 2 (Th-2) response, and larvae were apparently retained within granulomata in the liver, without obvious signs of destruction.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 5 , September 2016 , pp. 569 - 576
Copyright
Copyright © Cambridge University Press 2015 

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