Skip to main content Accessibility help
×
Home
Hostname: page-component-99c86f546-x5mqb Total loading time: 0.362 Render date: 2021-12-08T22:47:36.610Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam

Published online by Cambridge University Press:  05 April 2018

Q.P. Trinh*
Affiliation:
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
T.M.L. Le
Affiliation:
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
T.D. Nguyen
Affiliation:
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
H.T. Nguyen
Affiliation:
Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium
G. Liebanas
Affiliation:
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus ‘Las Lagunillas’ s/n, Edificio B3, 23071–Jaén, Spain
T.A.D. Nguyen
Affiliation:
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus ‘Las Lagunillas’ s/n, Edificio B3, 23071–Jaén, Spain
*
Author for correspondence: Q.P. Trinh, E-mail: tqphap@gmail.com

Abstract

The root-knot nematode species Meloidogyne daklakensis n. sp. was discovered on the roots of Robusta coffee (Coffea canephora Pierre ex A. Froehner) in Dak Lak Province, Vietnam. This species is characterized by the females having rounded or oval perineal patterns, smooth, regular, continuous striae, and reduced lateral lines. The dorsal arch is low, rounded and encloses a quite distinct vulva and tail tip. The stylet is normally straight with well-developed and posteriorly sloped knobs. The males have a rounded cap that extends posteriorly into the lip region. The procorpus is outlined distinctly, and is three times longer than the metacorpus. The metacorpus is ovoid, with a strong valve apparatus. The species closely resembles M. marylandi, M. naasi, M. ovalis, M. panyuensis, M. lopezi, M. mali and M. baetica in the perineal pattern of the females, and the morphology of the males and the second-stage juveniles. Nonetheless, it can be differentiated from other species by a combination of morphometric, morphological and molecular characteristics. Phylogenetic analysis was conducted based on the internal transcribed spacer (ITS) and 28S rDNA as well as the region between the cytochrome c oxidase I (COI) and cytochrome c oxidase II (COII) mitochondrial genes. Herein, this nematode is described, illustrated, and designated as a new species, Meloidogyne daklakensis sp. n., based on morphometric, morphological and molecular analyses.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abolafia, J (2015) A low-cost technique to manufacture a container to process meiofauna for scanning electron microscopy. Microscopy Research and Technique 78, 771776.CrossRefGoogle ScholarPubMed
Ahmed, M, van de Vossenberg, BTLH, Cornelisse, C and Karssen, G (2013) On the species status of the root-knot nematode Meloidogyne ulmi Palmisano & Ambrogioni, 2000 (Nematoda, Meloidogynidae). ZooKeys 362, 127.Google Scholar
Baidoo, R, Joseph, S, Mengistu, TM, Brito, JA, McSorley, R, Stamps, RH and Crow, WT (2016) Mitochondrial haplotype-based identification of root-knot nematodes (Meloidogyne spp.) on cut foliage crops in Florida. Journal of Nematology 48, 193202.CrossRefGoogle ScholarPubMed
Blaxter, ML (2001) Molecular analysis of nematode evolution. pp. 124 in Kennedy, MW and Harnett, W (Eds) Parasitic nematodes: molecular biology, biochemistry and immunology. Wallingford, UK, CABI.Google Scholar
Blok, VC and Powers, TO (2009) Biochemical and molecular identification. pp. 98112 in Perry, RN, Moens, M and Starr, J (Eds) Root knot nematodes. 1st edn. London, CABI.CrossRefGoogle Scholar
Blouin, MS (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology 32, 527531.CrossRefGoogle ScholarPubMed
Brito, J, Powers, TO, Mullin, PG, Inserra, RN and Dickson, DW (2004) Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. Journal of Nematology 36, 232240.Google ScholarPubMed
Carneiro, RMDG and Almeida, MRA (2001) Técnica de eletroforese usada no estudo de enzimas dos nematoides das galhas para identificação de espécies. Nematologia Brasileira 25, 3544.Google Scholar
Carneiro, RMDG and Cofcewics, ET (2008) Taxonomy of coffee parasitic root knot nematodes, Meloidogyne spp. pp. 87122 in Souza, RM (Ed.) Plant parasitic nematodes of coffee. 1st edn. Netherlands, Springer Netherlands.CrossRefGoogle Scholar
Carneiro, RMDG, Almeida, MRA and Quénéhervé, P (2000) Enzyme phenotypes of Meloidogyne spp populations. Nematology 2, 645654.CrossRefGoogle Scholar
Carneiro, RMDG, Randig, O, Almeida, MRA and Goncalves, W (2005) Identificacao e caracterizacao de species de Meloidogyne em cafeeiro nos Estados de Sao Paulo e Minas Gerais atraves dos fenotipos de esterase e SCAR–multiplex–PCR. Nematologia Brasiliera 29, 233241.Google Scholar
Castillo, P, Vovlas, N, Subbotin, S and Troccoli, A (2003) A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in southern Spain. Phytopathology 93, 10931102.CrossRefGoogle Scholar
Chitwood, BG (1949) Root-knot nematodes – Part I. A revision of the genus Meloidogyne Göldi, 1887. Proceedings of the Helminthological Society of Washington 16, 90104.Google Scholar
De Grisse, AT (1969) Redescription ou modification de quelques techniques utilisées dans l’étude de nématodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen Gent 34, 351369.Google Scholar
De Ley, P, Felix, MA, Frisse, LM, Nadler, SA, Sternberg, PW and Thomas, WK (1999) Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591612.Google Scholar
De Ley, IT, De Ley, P, Vierstraete, A, Karssen, G, Moens, M and Vanfleteren, J. (2002) Phylogenetic analyses of Meloidogyne small subunit rDNA. Journal of Nematology 34, 319327.Google ScholarPubMed
Eisenback, JD (1985) Detailed morphology and anatomy of second-stage juveniles, males, and females of the genus Meloidogyne (root-knot nematodes). pp. 4778 in Sasser, JN and Carter, CC (Eds) An advanced treatise on Meloidogyne, Vol. 1. Raleigh, North Carolina State University Graphics.Google Scholar
Franklin, MT (1965) A root-knot nematode, Meloidogyne naasi n. sp., on field crops in England and Wales. Nematologica 11, 7986.CrossRefGoogle Scholar
Göldi, EA (1887) Relatorio sobre a molestia do cafeeiro na provincia do Rio de Janeiro. Extrahido do VIII Vol. dos. Archivos do Museu Nacional, Rio de Janeiro. Imprensa Nacional, pp. 1121.Google Scholar
Hall, TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.Google Scholar
Hartman, KM and Sasser, JN (1985) Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. pp. 6977 in Barker, KR, Carter, CC and Sasser, JN (Eds) An advanced treatise on Meloidogyne. Vol. II: Methodology. A cooperative publication of the Department of Plant Pathology and the United States Agency for International Development. Raleigh, North Carolina State University Graphics.Google Scholar
Herrera, I, Bryngelsson, T, Monzón, A and Geleta, M (2011) Identification of coffee root-knot nematodes based on perineal pattern, SCAR markers and nuclear ribosomal DNA sequences. Nematologia Mediterranea 39, 101110.Google Scholar
Hewlett, TE and Tarjan, AC (1983) Monographs. Synopsis of the genus Meloidogyne Goeldi, 1887. Nematropica 13, 79102.Google Scholar
Holterman, HM, Oggenfuss, M, Kiewnick, S and Frey, JE (2009) Variation present in the COI gene of Meloidogyne and its potential for molecular identification. Journal of Nematology 41, 340.Google Scholar
Hugall, A, Moritz, C, Stanton, J and Wolstenholme, DR (1994) Low, but strongly structured mitochondrial DNA diversity in root knot nematodes (Meloidogyne). Genetics 136, 903912.Google Scholar
Humphreys-Pereira, DA, Flores-Chaves, L, Gómez, M, Salazar, L, Gómez-Alpízar, L and Elling, AA (2014) Meloidogyne lopezi n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with coffee (Coffea arabica L.) in Costa Rica, its diagnosis and phylogenetic relationship with other coffee-parasitising Meloidogyne species. Nematology 16, 643661.CrossRefGoogle Scholar
Hunt, DJ and Handoo, ZA (2009) Taxonomy, identification and principal species. pp. 5597 in Perry, RN, Moens, M and Starr, JL (Eds) Root-knot nematodes. Wallingford, UK, CABI.CrossRefGoogle Scholar
Hussey, RS and Janssen, GJW (2002) Root-knot nematodes: Meloidogyne species. pp. 4370 in Starr, JL, Cook, R and Bridge, J (Eds) Plant resistance to parasitic nematodes. Wallingford, UK, CABI.CrossRefGoogle Scholar
Itoh, Y, Ohshima, Y and Ichinohe, M (1969) A root-knot nematode, Meloidogyne mali n. sp., on apple-tree from Japan (Tylenchida: Heteroderidae). Applied Entomology and Zoology 4, 194202.Google Scholar
Janssen, T, Karssen, G, Verhaeven, M, Coyne, D and Bert, W (2016) Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports 6. doi: 10.1038/srep22591.CrossRefGoogle ScholarPubMed
Janssen, T, Karssen, G, Topalović, O, Coyne, D and Bert, W (2017) Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis. PLoS ONE 12(3), e0172190.CrossRefGoogle ScholarPubMed
Jepson, SB (1987) Identification of root-knot nematodes (Meloidogyne species). Wallingford, UK, CABI, 265 pp.Google Scholar
Jepson, SB and Golden, AM (1987) Meloidogyne marylandi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing grasses. pp. 263265 in Jepson, SB (Ed.) Identification of root-knot nematodes (Meloidogyne species). Wallingford, UK, CABI.Google Scholar
Karssen, G (2002) The plant-parasitic nematode genus Meloidogyne Göldi, 1892 (Tylenchida) in Europe. Leiden, Netherlands, Brill, 157 pp.Google Scholar
Kazachenko, IP and Mukhina, TI (2013) Root-knot nematodes of genus Meloidogyne Goeldi (Tylenchida: Meloidogynidae) of the world. Vladivostok, Russia, Institute of Biology and Soil Sciences, 306 pp.Google Scholar
Kiewnick, S, Holterman, M, van den Elsen, S, van Megen, H, Frey, JE and Helder, J (2014) Comparison of two short DNA barcoding loci (COI and COII) and two longer ribosomal DNA genes (SSU & LSU rRNA) for specimen identification among quarantine root-knot nematodes (Meloidogyne spp.) and their close relatives. European Journal of Plant Pathology 140, 97110.CrossRefGoogle Scholar
Kofoid, CA and White, WA (1919) A new nematode infection of man. Journal of the American Medical Association 72, 567569.CrossRefGoogle Scholar
Landa, BB, Rius, JEP, Vovlas, N, Carneiro, RMDG, Maleita, CMN, Abrantes, IMO and Castillo, P (2008) Molecular characterization of Meloidogyne hispanica (Nematoda, Meloidogynidae) by phylogenetic analysis of genes within the rDNA in Meloidogyne spp. Plant Disease 92, 11041110.CrossRefGoogle ScholarPubMed
Liao, J, Yang, W, Feng, Z and Karssen, G (2005) Description of Meloidogyne panyuensis sp. n. (Nematoda: Meloidogynidae), parasitic on peanut (Arachis hypogaea L.) in China. Russian Journal of Nematology 13, 107114.Google Scholar
Nguyen, NC and Nguyen, VT (2000) Plant parasitic nematodes in Vietnam. Hanoi, Vietnam, Science and Technology Publishing House, 403 pp. (in Vietnamese).Google Scholar
Nguyen, NC and Nguyen, VT (2001) Plant parasitic nematodes associated with coffee crop in some North and Tay Nguyen Highland provinces of Vietnam. pp. 188195 in Selected works on ecological and bioresource studies (1996–2000). Hanoi, Vietnam, Hanoi Agricultural Publishing House (in Vietnamese).Google Scholar
Oliveira, CMG, Monteiro, AR and Blok, VC (2011) Morphological and molecular diagnostics for plant parasitic nematodes: working together to get the identification done. Tropical Plant Pathology 36, 6573.Google Scholar
Onkendi, EM and Moleleki, LN (2013) Distribution and genetic diversity of root-knot nematodes (Meloidogyne spp.) in potatoes from South Africa. Plant Pathology 62, 11841192.CrossRefGoogle Scholar
Pagan, C, Coyne, D, Carneiro, R, Kariuki, G, Luambano, N, Affokpon, A and Williamson, VM (2015) Mitochondrial haplotype-based identification of ethanol-preserved root-knot nematodes from Africa. Phytopathology 105, 350357.CrossRefGoogle ScholarPubMed
Perry, R and Moens, M (2013) Plant nematology. 2nd edn. Wallingford, UK, CABI, 568 pp.CrossRefGoogle Scholar
Plantard, O, Picard, D, Valette, S, Scurrah, M, Grenier, E and Mugniéry, D (2008) Origin and genetic diversity of Western European populations of the potato cyst nematode (Globodera pallida) inferred from mitochondrial sequences and microsatellite loci. Molecular Ecology 17, 22082218.CrossRefGoogle ScholarPubMed
Powers, TO and Harris, TS (1993) A polymerase chain reaction method for identification of five major Meloidogyne species. Journal of Nematology 25, 16.Google ScholarPubMed
Riffle, JW (1963) Meloidogyne ovalis (Nematoda: Heteroderidae), a new species of root-knot nematode. Proceedings of the Helminthological Society of Washington 30, 287292.Google Scholar
Seinhorst, JW (1962) On the killing, fixation and transferring to glycerin of nematodes. Nematologica 8, 2932.CrossRefGoogle Scholar
Souza, RM (2008) Plant-parasitic nematodes of coffee. Berlin, Germany, Springer International Publishing, 340 pp.CrossRefGoogle Scholar
Tamura, K, Stecher, G, Peterson, D, Filipski, A and Kumar, S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 27252729.CrossRefGoogle ScholarPubMed
Tigano, MS, Carneiro, RMDG, Jeyaprakash, A, Dickson, DW and Adams, BJ (2005) Phylogeny of Meloidogyne spp. based on 18S rDNA and the intergenic region of mitochondrial DNA sequences. Nematology 7, 851862.Google Scholar
Treub, M (1885) Onderzoekingen over seren–ziek suikerriet gedaan in ‘slands plantentuin te Buitenzorg. Meded. Pl. Tuin, Batavia 2.Google Scholar
Trinh, PQ, de la Peña, E, Nguyen, CN, Nguyen, HX and Moens, M (2009) Plant-parasitic nematodes associated with coffee in Vietnam. Russian Journal of Nematology 17, 7382.Google Scholar
Trinh, PQ, Pham, TKD and Nguyen, NC (2013) Emerging Meloidogyne species (root-knot nematodes) threats to coffee in the Western Highlands in Vietnam. Proceedings of the 2nd VAST–KAST workshop on biodiversity and bop–active compounds, 313–318.Google Scholar
Trudgill, DL and Blok, VC (2001) Apomictic, polyphagous root-knot nematodes: exceptionally successful and damaging biotrophic root pathogens. Annual Review of Phytopathology 39, 5377.CrossRefGoogle ScholarPubMed
Vrain, TC, Wakarchuk, DA, Lévesque, AC and Hamilton, RI (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563573.Google Scholar
Wesemael, WML, Viaene, N and Moens, M (2011) Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology 13, 316.CrossRefGoogle Scholar
Whitehead, AG and Hemming, JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55, 2538.CrossRefGoogle Scholar
Ye, W, Zeng, Y and Kerns, J (2015) Molecular characterisation and diagnosis of root-knot nematodes (Meloidogyne spp.) from turfgrasses in North Carolina, USA. PLoS ONE 10(11), e0143556.CrossRefGoogle ScholarPubMed
10
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *