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Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

  • E. Hernández-Cruz (a1), J.J. González-Cabriales (a1), C. Ordaz-Pichardo (a1), N.I. de la Cruz-Hernández (a2) and G.H. Flores-Gutiérrez (a1)...

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

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P. Agudelo , D. Botero & L.G. Palacio (2005) Evaluation of the ELISA method for diagnosis of human cysticercosis in an endemic region. Biomedica 25, 488495.

R. Biswas , S.C. Parija & S.K. Narayan (2004) Dot-ELISA for the diagnosis of neurocysticercosis. Revista do Instituto de Medicina Tropical de São Paulo 46, 249252.

M.M. Bradford (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248254.

F. Bucardo , A. Meza-Lucas , F. Espinoza , R.C. Garcia-Jeronimo , R. Garcia-Rodea & D. Correa (2005) The seroprevalence of Taenia solium cysticercosis among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting. Annals of Tropical Medicine and Parasitology 99, 4145.

D. Correa , M.A. Sandoval , L.J. Harrison , R.M. Parkhouse , A. Plancarte , A. Meza-Lucas & A. Flisser (1989) Human neurocysticercosis: comparison of enzyme immunoassay capture techniques based on monoclonal and polyclonal antibodies for the detection of parasite products in cerebrospinal fluid. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 814816.

A. Fleury , B. Bouteille , E. Garcia , C. Marquez , P.M. Preux , F. Escobedo , J. Sotelo & M. Dumas (2001) Neurocysticercosis: validity of ELISA after storage of whole blood and cerebrospinal fluid on paper. Tropical Medicine and International Health 6, 688693.

M. Kaur , K. Joshi , N.K. Ganguly , R.C. Mahajan & N. Malla (1995) Evaluation of the efficacy of albendazole against the larvae of Taenia solium in experimentally infected pigs, and kinetics of the immune response. International Journal for Parasitology 25, 14431450.

G. Nsengiyumva , M. Druet-Cabanac , B. Ramanankandrasana , B. Bouteille , L. Nsizabira & P.M. Preux (2003) Cysticercosis as a major risk factor for epilepsy in Burundi, east Africa. Epilepsia 44, 950955.

N.S. Odashima , O.M. Takayanagui & J.F. Figueiredo (2002) Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis. Arquivos de Neuro-psiquiatria 60, 400405.

R.H. Peralta , A.J. Vaz , A. Pardini , H.W. Macedo , L.R. Machado , S.G. De Simone & J.M. Peralta (2002) Evaluation of an antigen from Taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis. Acta Tropica 83, 159168.

R. Rodriguez-Canul , A. Fraser , J.C. Allan , J.L. Dominguez-Alpizar , F. Argaez-Rodriguez & P.S. Craig (1999) Epidemiological study of Taenia solium taeniasis/cysticercosis in a rural village in Yucatan state, Mexico. Annals of Tropical Medicine and Parasitology 93, 5767.

N. Rosas , J. Sotelo & D. Nieto (1986) ELISA in the diagnosis of neurocysticercosis. Archives of Neurology 43, 353356.

E. Santellano-Estrada , F. Infante , E. Díaz-Aparicio & G.H. Flores-Gutiérrez (2004) Use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis. Veterinary Research Communications 28, 2731.

A.J. Vaz , A.W. Ferreira , M.E. Camargo , P.M. Nakamura & E.D. Camargo (1990) DOT-ELISA for detection of anti-Cysticercus cellulosae antibodies in human cerebrospinal fluid using a new solid-phase (resin-treated polyester fabrics). Preliminary report. Revista do Instituto de Medicina Tropical de São Paulo 32, 355359.

A.J. Vaz , P.M. Nakamura , M.E. Camargo , E.D. Camargo & A.W. Ferreira (1996) Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens. Revista do Instituto de Medicina Tropical de São Paulo 38, 391396.

R. Vega , D. Pinero , B. Ramanankandrasana , M. Dumas , B. Bouteille , A. Fleury , E. Sciutto , C. Larralde & G. Fragoso (2003) Population genetic structure of Taenia solium from Madagascar and Mexico: implications for clinical profile diversity and immunological technology. International Journal for Parasitology 33, 14791485.

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Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
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