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Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

  • A. Ito (a1), M.I. Putra (a2), R. Subahar (a3), M.O. Sato (a1), M. Okamoto (a4), Y. Sako (a1), M. Nakao (a1), H. Yamasaki (a1), K. Nakaya (a5), P.S. Craig (a6) and S.S. Margono (a3)...

Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

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Bowles, J. & McManus, D.P. (1994) Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. American Journal of Tropical Medicine and Hygiene 50, 3344.
Ito, A., Plancarte, A., Ma, L., Kong, Y., Flisser, A., Cho, S.Y., Liu, Y.H., Kamhawi, S., Lightowlers, M.W. & Schantz, P.M. (1998) Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291294.
Ito, A., Plancarte, A., Nakao, M., Nakaya, K., Ikejima, T., Piao, Z.X., Kanazawa, T. & Margono, S.S. (1999) ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium. Journal of Helminthology 73, 363365.
Ito, A., Sako, Y., Nakao, M. & Nakaya, K. (2002a) Neurocysticercosis in Asia: serology/seroepidemiology in humans and pigs. pp. 2531 in Craig, P.S. & Pawlowski, Z. (Eds) Cestode zoonoses: echinococcosis and cysticercosis – anemergentandglobalproblem. Amsterdam, IOS Press.
Ito, A., Nakao, M., Okamoto, M., Sako, Y. & Yamasaki, H. (2002b) Mitochondrial DNA of Taenia solium: from basic to applied science. Chapter 5 in Singh, G. & Prabhakar, S. (Eds) Taenia solium cysticercosis: from basic to clinical science. Wallingford, Oxon, CABI Publishing.
McManus, D.P. (1997) Molecular genetic variation in Echinococcus and Taenia: an update. Southeast Asian Journal of Tropical Medicine and Public Health 28 (Suppl 1), 110116.
Meijer, W.C.P. (1933) Extracten unit de maand – en jaarverslagen der gouvernements en provinciale veeartsen. No. 62, I. Cysticercosis. II. Trichinosis. Nederlandsch-Indische Bladen voor Diergeneeskunde en Dierenteelt 14 (3), 165175 (see Helminthological Abstracts 1933, 2, 88).
Nakao, M., Okamoto, M., Sako, Y., Yamasaki, H., Nakaya, K. & Ito, A. (2002) A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide. Parasitology 124, 657662.
Okamoto, M., Bessho, Y., Kamiya, M., Kurosawa, T. & Horii, T. (1995) Phylogenetic relationships within Taenia taeniaeformis variants and other taeniid cestodes inferred from the nucleotide sequences of the cytochrome c oxidase subunit I gene. Parasitology Research 81, 451458.
Okamoto, M., Nakao, M., Sako, Y. & Ito, A. (2001) Molecular variation of Taenia solium in the world. Southeast Asian Journal of Tropical Medicine and Public Health 32 (Suppl 2), 9093.
Okolo, M.I.O. (1986) Observations on Cysticercus cellulosae in the flesh of rural dogs. International Journal of Zoonoses 13, 286289.
Sako, Y., Nakao, M., Ikejima, T., Piao, Z.X., Nakaya, K. & Ito, A. (2000) Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. Journal of Clinical Microbiology 38, 44394444.
Simanjuntak, G.M., Margono, S.S., Okamoto, M. & Ito, A. (1997) Taeniasis/cysticercosis in Indonesia as an emerging disease. Parasitology Today 13, 321323.
Subahar, R., Hamid, A., Purba, W., Wandra, T., Karma, C., Sako, Y., Margono, S.S., Craig, P.S. & Ito, A. (2001) Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District. Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 388390.
Theis, J.H., Cleary, M., Syvanen, M., Gilson, A., Swift, P., Banks, J. & Johnson, E. (1996) DNA-confirmed Taenia solium cysticercosis in black bears (Ursus americanus ) from California. American Journal of Tropical Medicine and Hygiene 55, 456458.
Verster, A. (1967) Redescription of Taenia solium Linnaeus, 1758 and Taenia saginata Goeze, 1782. Zeitschrift für Parasitenkunde 29, 313328.
Wandra, T., Subahar, R., Simanjuntak, G.M., Margono, S.S., Suroso, T., Okamoto, M., Nakao, M., Sako, Y., Nakaya, K., Schantz, P.M. & Ito, A. (2000) Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia, 1991–95. Transactions of the Royal Society of Tropical Medicine and Hygiene 94, 4650.
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Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
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