Hostname: page-component-8448b6f56d-tj2md Total loading time: 0 Render date: 2024-04-25T02:00:21.668Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of infection with Angiostrongylus cantonensis on the circulating haemocyte population and the haematopoietic organ of the host snail M-line Biomphalaria glabrata

Published online by Cambridge University Press:  05 June 2009

S. Noda  and
A. Sato
S. Noda
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Kagoshima University, Usuki, Kagoshima 890, Japan
A. Sato
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Kagoshima University, Usuki, Kagoshima 890, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

The number of circulating haemocytes, the size of the haematopoietic organ, and the size of haemocyte capsules around the parasite were studied in M-line Biomphalaria glabrata snails exposed to 100 or 400 first-stage larvae of Angiostrongylus cantonensis. The number of haemocytes in exposed snails increased significantly at 1 day post-exposure, decreased to control value, and then increased again. The decrease in number of circulating haemocytes is probably due to the removal of cells from the circulation to participate in encapsulation of larvae. The majority of circulating haemocytes in M-line B. glabrata are fully-spread granulocytes, which increase significantly in number in snails following exposure to A. cantonensis larvae. However, populations of partially-spread granulocytes, round cells, hyalinocytes and miscellaneous haemocytes were relatively constant. The size of capsules around the parasite increased during the 42-day interval of the experiment. The haematopoietic organ increased in size in response to infection.

Keywords

Angiostrongylus cantonensisBiomphalaria glabratahaemocytehaematopoietic organimmunity
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 3 , September 1990 , pp. 239 - 247
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abudul-Salam, J. M. & Michelson, E. H. (1980) Biomphalaria glabrata amoebocytes: Effect of Schistosoma mansoni infection on in vitro phagocytosis. Journal of Invertebrate Pathology, 35, 241–248.CrossRefGoogle Scholar
Alicata, J. E. & Jindrak, K. (1970) Angiostrongylosis in the Pacific and Southeast Asia. Charles C. Thomas: Springfield, Illinois. 105 pp.Google Scholar
Cheng, T. C. & Garrabrant, T. A. (1977) Acid phosphatase in granulocyte capsules formed in strains of Biomphalaria glabrata totally and partially resistant to Schistosoma mansoni. International Journal for Parasitology, 7, 467–472.CrossRefGoogle ScholarPubMed
Granath, W. O. Jr & Yoshino, T. P. (1983) Lysosomal enzyme activities in susceptible and refractory strains of Biomphalaria glabrata during the course of infection withSchistosoma mansoni. Journal of Parasitology, 69, 1018–1026.CrossRefGoogle ScholarPubMed
Granath, W. O. Jr & Yoshino, T. P. (1985) Biomphalaria glabrata (Gastropoda): Effect of urethane on the morphology and function of hemocytes, and on susceptibility to Schistosoma mansoni (Trematoda). Journal of Invertebrate Pathology, 45, 324–330.CrossRefGoogle ScholarPubMed
Harris, K. R. (1975) The fine structure of encapsulation in Biomphalaria glabrata. Annals of the New York Academy of Sciences, 266, 446–464.CrossRefGoogle ScholarPubMed
Harris, K. R. & Cheng, T. C. (1975a) The encapsulation process in Biomphalaria glabrata experimentally infected with the metastrongylid Angiostrongylus cantonensis: Light microscopy. International Journal for Parasitology, 5, 521–528.CrossRefGoogle ScholarPubMed
Harris, K. R. & Cheng, T. C. (1975b) The encapsulation process in Biomphalaria glabrata experimentally infected with metastrongylid Angiostrongylus cantonensis: Enzyme histochemistry. Journal of Invertebrate Pathology, 26, 367–374.CrossRefGoogle ScholarPubMed
Jeong, K. H., Lie, K. J. & Heyneman, D. (1984) An ultrastructural study on ventricular capsule reactions in Biomphalaria glabrata exposed to irradiated echinostome parasites. International Journal for Parasitology, 14, 127–133.CrossRefGoogle Scholar
Lie, K. J. (1982) Survival of Schistosoma mansoni and other trematode larvae in the snail Biomphalaria glabrata. A discussion of the interference hypothesis. Tropical and Geographical Medicine, 34, 111–122.Google Scholar
Lie, K. J., Heyneman, D. & Yau, P. (1975) The origin of amebocytes inBiomphalaria glabrata. Journal of Parasitology, 63, 574–576.CrossRefGoogle Scholar
Loker, E. S. & Bayne, C. J. (1986) Immunity to trematode larvae in the snail Biomphalaria. Symposium of the Zoological Society of London, 56, 199–220.Google Scholar
Loker, E. S., Bayne, C. J., Buckley, P. M. & Kruse, K. T. (1982) Ultrastructure of encapsulation of Schistosoma mansoni mother sporocysts by hemocytes of juveniles of the 10-R2 strain ofBiomphalaria glabrata. Journal of Parasitology, 68, 84–94.CrossRefGoogle Scholar
Loker, E. S., Cimino, D. F., Stryker, G. A. & Hertel, L. A. (1987) The effect of size of M-line Biomphalaria glabrata on the course of development ofEchinostoma paraensei. Journal of Parasitology, 73, 1090–1098.CrossRefGoogle ScholarPubMed
Loker, E. S. & Hertel, L. A. (1987) Alterations in Biomphalaria glabrata plasma induced by infection with the digenetic trematode Echinostoma paraensi. Journal of Parasitology, 73, 503–513.CrossRefGoogle Scholar
Noda, S. & Loker, E. S. (1989a) Effects of infection with Echinostoma paraensei on the circulating haemocyte population of the host snail Biomphalaria glabrata. Parasitology, 98, 35–41.CrossRefGoogle ScholarPubMed
Noda, S. & Loker, E. S. (1989b) Phagocytic activity of hemocytes of M-line Biomphalaria glabrata snails: Effect of exposure to the trematodeEchinostoma paraensei. Journal of Parasitology, 75, 261–269.CrossRefGoogle Scholar
Noda, S., Uchikawa, R., Matayoshi, S., Watanabe, Y. & Sato, A. (1987) Observations on the transmission of Angiostrongylus cantonensis from snail to rodent. Journal of Helminthology, 61, 241–246.CrossRefGoogle ScholarPubMed
Otsuru, M. (1977) Angiostrongylus cantonensis: In: Animals of medical importance in the Nansei Islands in Japan (editor Sasa, M.et al.) pp. 343–374. Shinjuku Shobo: Tokyo.Google Scholar
Richards, C. S. & Merritt, J. W. (1967) Studies on Angiostrongylus cantonensis in molluscan intermediate hosts. Journal of Parasitology, 53, 382–388.CrossRefGoogle ScholarPubMed
Sminia, T. (1972) Structure and function of blood and connective tissue cells of the fresh water pulmonate Lymnaea stagnalis studied by electron microscopy and enzyme histochemistry. Zeitschrift für Zellforschung und mikroskopische Anatomie, 130, 497–526.CrossRefGoogle ScholarPubMed
Stumpf, J. L. & Gilbertson, D. E. (1978) Hemocytes of Biomphalaria glabrata: Factors affecting variability. Journal of Invertebrate Pathology, 32, 177–181.CrossRefGoogle ScholarPubMed
Sullivan, J. T., Cheng, T. C. & Howland, K. H. (1984) Mitotic responses of the anterior pericardial wall of Biomphalaria glabrata (Mollusca) subjected to challenge. Journal of Invertebrate Pathology, 44, 114–116.CrossRefGoogle Scholar
Ulmer, M. J. (1970) Notes on rearing snails in the laboratory. In: Experiments and techniques in Parasitology (Editors Macinnis, A. J. and Voge, M) pp. 143–144. W. H. Freeman: San Francisco.Google Scholar
Yoshino, T. P. (1981) Comparison of concanavalin A-reactive determinants of hemocytes of two Biomphalaria glabrata snail stocks: Receptor binding and redistribution. Development and Comparative Immunology, 5, 229–239.CrossRefGoogle ScholarPubMed