Skip to main content Accessibility help
×
×
Home

Expression of Hsp90, Hsp70 and Hsp60 in Trichinella species exposed to oxidative shock

  • J. Martinez (a1), J. Perez-Serrano (a1), W.E. Bernadina (a2) and F. Rodriguez-Caabeiro (a1)
Abstract

Stress response and phosphorylation of heat shock proteins (HSPs) 60, 70 and 90 were studied in Trichinella nativa, T. nelsoni, T. pseudospiralis and T. spiralis larvae at 30-min intervals following exposure to 20, 100 and 200 mM H2O2. There was a time- and dose-dependent differential survival for the infective stage larvae (L1) of these four Trichinella species. Immunoblotting analysis revealed that constitutive Hsp60 and Hsp70, but not Hsp90, from test Trichinella species are constitutively phosphorylated on serine/threonine residues as they converted to forms with increased sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) mobility by treatment with alkaline phosphatase. After exposure to H2O2, while there was a time-related occurrence of the three HSPs with decreased SDS–PAGE mobility, these HSPs were insensitive to alkaline phosphatase except in the case of exposure to 20 mM H2O2 for Hsp60 from all Trichinella species and Hsp70 from T. spiralis and T. nelsoni. The synthesis of HSPs forms with decreased SDS–PAGE mobility is a susceptibility signal because the lower concentration of peroxide (20 mM) did not cause a decrease on HSPs SDS–PAGE mobility in T. spiralis and T. nelsoni, the two more resistant selected Trichinella species.

Copyright
Corresponding author
*Fax: 34 91 8854663 E-mail: francisco.martinez@uah.es.
References
Hide All
Bell, R.G. & Wang, C.H. (1987) The Trichinella spiralis newborn larvae: production, migration and immunity in vivo. Wiadomosci Parazytologiczne 4–5, 453478.
Bell, R.G. (1998) The generation and expression of immunity to Trichinella spiralis in laboratory rodents. Advances in Parasitology 41, 149217.
Brand, T., Weistein, P., Mehlan, B. & Weinbach, E. (1952) Observation on the metabolism of bacteria free larvae of Trichinella spiralis. Experimental Parasitology 1, 245255.
Bruschi, F., Carulli, G., Azzarà, A., Homan, W., Minnucci, S., Rizzuti-Gillaci, A., Sbraa, S. & Angiolini, C. (2000) Inhibitory effects of human neutrophil functions by the 45–kD glycoprotein derived from the parasitic nematode Trichinella spiralis. International Archives of Allergy and Applied Immunology 122, 5865.
Bukau, B. & Horwich, A.L. (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92, 351366.
Buys, J., Wever, R., Van Stigt, R. & Ruitenberg, E.J. (1981) The killing of newborn larvae of Trichinella spiralis by eosinophil peroxidase in vitro. European Journal of Immunology 11, 843845.
Edelman, A.M. & Blumenthal, D.K. (1987) Protein serine/threonine kinases. Annual Review of Biochemistry 56, 567613.
Hadas, E., Rodriguez-Caabeiro, F. & Jimenez-Gonzalez, A. (1994) Oxidant defenses in different isolates of Trichinella. Acta Parasitologica 39, 3236.
Jenkins, D.C. & Carrington, T.S. (1981) An in vitro screening test for compounds active againts the parenteral stages of Trichinella spiralis. Tropenmedizin und Parasitologie 32, 3134.
Kazura, J.W. & Grove, D.I. (1978) Stage-dependent antibody-dependent eosinophil-mediated destruction of Trichinella spiralis. Nature 274, 588589.
Kazura, J.W. & Meshnick, S.R. (1984) Scavenger enzymes and resistance to oxygen mediated damage in Trichinella spiralis. Molecular and Biochemical Parasitology 10, 110.
La Rosa, G., Pozio, E., Rossi, P. & Murrell, K.D. (1992) Allozyme analysis of Trichinella isolates from various host species and geographical regions. Journal of Parasitology 78, 641646.
Lindquist, S. (1986) The heat shock response. Annual Review of Biochemistry 55, 11511191.
Martinez, J., Perez-Serrano, J., Bernadina, W.E. & Rodriguez-Caabeiro, F. (1999) In vitro stress response to elevated temperature, hydrogen peroxide and mebendazole in Trichinella spiralis muscle larvae. International Journal for Parasitology 29, 14571464.
Martinez, J., Perez-Serrano, J., Bernadina, W.E. & Rodriguez-Caabeiro, F. (2000) Detection of heat shock protein-70 from Trichinella spiralis larvae using a modification of the routine Western blotting procedure. Journal of Parasitology 86, 637639.
Mehlen, P., Schultze-Osthoff, K. & Arrigo, A.P. (1996) Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 blocks Fas/APO-1 and staurosporine-induced cell death. Journal of Biological Chemistry 271, 1651016514.
Murray, E.S., Smith, V.P., Thomas, G.R. & Gounaris, K. (1998) Resistance of filarial nematode parasites to oxidative stress. International Journal for Parasitology 28, 13151332.
Ou, X., Thomas, G.R., Chacón, M.R., Tang, L. & Selkirk, M.E. (1995) Brugia malayi: differential susceptibility to a metabolism of hydrogen peroxide in adults and microfilariae. Experimental Parasitology 80, 530540.
Polla, B.S., Perin, M. & Pizurki, L. (1993) Regulation and function of stress proteins in allergy and inflammation. Clinical and Experimental Allergy 23, 548556.
Pozio, E., La Rosa, G. & Rossi, P. (1989) Present status of the taxonomy of the Trichinella genus. Wiadomosci Parazytologiczne 5, 483484.
Pozio, E., La Rosa, G., Murell, K.D. & Lichtenfels, J.R. (1992) Taxonomic revision of the genus Trichinella. Journal of Parasitology 78, 654659.
Schlesinger, M.J. (1990) Heat shock proteins. Journal of Biological Chemistry 265, 1211112114.
Wakelin, D. (1984) Evasion of the immune response: survival within low responder individuals of the host population. Parasitology 88, 639657.
Wang, C.H. & Bell, R.G. (1988) Antibody-mediated in-vivo cytotoxicity to Trichinella spiralis newborn larvae in immune rats. Parasite Immunology 10, 293308.
Weiss, S.J., Test, S.T., Eckmann, C.M., Roos, D. & Regiani, S. (1986) Brominating oxidants generated by human eosinophils. Science 234, 200203.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
  • URL: /core/journals/journal-of-helminthology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed