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Immunisation of baboons against Schistosoma mansoni using irradiated S. mansoni cercariae and schistosomula and non-irradiated S. rodhaini cercariae

Published online by Cambridge University Press:  05 June 2009

M. G. Taylor
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
E. R. James
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
G. S. Nelson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
Q. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
B. J. Andrews
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
A. R. Dobinson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
G. Webbe
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.

Abstract

In an attempt to develop a non-pathogenic procedure for immunising baboons against S. mansoni, groups of five baboons were exposed to three doses of 5000 6 Kr-irradiated S. mansoni cercariae or to similar numbers of normal S. rodhaini cercariae and challenged at week 15 with 500 normal S. mansoni cercariae. Faecal egg counts, worm and tissue egg counts, and histopathological examination, showed that neither of the immunising schedules had produced significant protection. In the second experiment baboons were injected by the intramuscular route with 31000 schistosomula of S. mansoni in three doses and the irradiation dose was reduced to near the minimum required for worm sterilisation (2·1–2·4 Kr). Challenge with 3500 normal cercariae of S. mansoni 21 weeks after the first immunising dose again showed no significant protection, although reductions of 20–30% were found, in egg and worm counts resulting from the challenge. These results indicate that it may be difficult to develop an effective live vaccine for S. mansoni unless the antigenicity of the immunising larvae can be greatly increased.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1976

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References

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