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Mixed infections with Opisthorchis viverrini and intestinal flukes in residents of Vientiane Municipality and Saravane Province in Laos

  • J.-Y. Chai (a1), J.-H. Park (a1), E.-T. Han (a2), S.-M. Guk (a1), E.-H. Shin (a1), A. Lin (a1), J.-L. Kim (a1), W.-M. Sohn (a3), T.-S. Yong (a4), K.S. Eom (a5), D.-Y. Min (a6), E.-H. Hwang (a7), B. Phommmasack (a8), B. Insisiengmay (a8) and H.-J. Rim (a9)...

Faecal examinations for helminth eggs were performed on 1869 people from two riverside localities, Vientiane Municipality and Saravane Province, along the Mekong River, Laos. To obtain adult flukes, 42 people positive for small trematode eggs (Opisthorchis viverrini, heterophyid, or lecithodendriid eggs) were treated with a 20–30 mg kg−1 single dose of praziquantel and purged. Diarrhoeic stools were then collected from 36 people (18 in each area) and searched for helminth parasites using stereomicroscopes. Faecal examinations revealed positive rates for small trematode eggs of 53.3% and 70.8% (average 65.2%) in Vientiane and Saravane Province, respectively. Infections with O. viverrini and six species of intestinal flukes were found, namely, Haplorchis taichui, H. pumilio, H. yokogawai, Centrocestus caninus, Prosthodendrium molenkampi, and Phaneropsolus bonnei. The total number of flukes collected and the proportion of fluke species recovered were markedly different in the two localities; in Vientiane, 1041 O. viverrini (57.8 per person) and 615 others (34.2 per person), whereas in Saravane, 395 O. viverrini (21.9 per person) and 155207 others (8622.6 per person). Five people from Saravane harboured no O. viverrini but numerous heterophyid and/or lecithodendriid flukes. The results indicate that O. viverrini and several species of heterophyid and lecithodendriid flukes are endemic in these two riverside localities, and suggest that the intensity of infection and the relative proportion of fluke species vary by locality along the Mekong River basin.

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Chai, J.Y. & Hongvanthong, B. (1998) A small-scale survey of intestinal helminthic infections among the residents near Pakse, Laos. Korean Journal of Parasitology 36, 5558.
Chai, J.Y. & Lee, S.H. (2002) Food-borne intestinal trematode infections in the Republic of Korea. Parasitology International 51, 129154.
Chai, J.Y., Murrell, K.D. & Lymbry, A. (2005) Fishborne parasitic zoonoses: status and issues. International Journal for Parasitology (in press).
Ditrich, O., Scholtz, T. & Giboda, M. (1990) Occurrence of some medically important flukes (Trematoda: Opisthorchiidae and Heterophyidae) in Nam Ngum water reservoir. Southeast Asian Journal of Tropical Medicine and Public Health 21, 482488.
Ditrich, O., Giboda, M., Scholtz, T. & Beer, S.A. (1992) Comparative morphology of eggs of the Haplorchiinae (Trematoda: Heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. Folia Parasitologica 39, 123132.
Giboda, M., Ditrich, O., Scholtz, T., Viengsay, T. & Bouaphanh, S. (1991) Human Opisthorchis and Haplorchis infections in Laos. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 538540.
Kaewkes, S., Elkins, D.B., Sithithaworn, P. & Haswell-Elkins, M.R. (1991) Comparative studies on the morphology of the eggs of Opisthorchis viverrini and lecithodendriid trematodes. Southeast Asian Journal of Tropical Medicine and Public Health 22, 623630.
Kobayashi, J., Vannachone, B., Xeutvongsa, A., Manivong, K., Ogawa, S., Sato, Y. & Pholsena, K. (1996) Prevalence of intestinal parasitic infection among children in two villages in Lao PDR. Southeast Asian Journal of Tropical Medicine and Public Health 27, 562565.
Kobayashi, J., Vannachone, B., Sato, Y., Manivong, K., Nambanya, S. & Inthakone, S. (2000) An epidemiological study on Opisthorchis viverrini infection in Lao villages. Southeast Asian Journal of Tropical Medicine and Public Health 31, 128132.
Manning, G.S. & Lertprasert, P. (1973) Studies on the life cycle of Phaneropsolus bonnei and Prosthodendrium molenkampi in Thailand. Annals of Tropical Medicine and Parasitology 67, 361365.
Manning, G.S., Lertprasert, P., Watanasirmkit, K. & Chetty, C. (1971) A description of newly discovered intestinal parasites endemic to northeastern Thailand. Journal of the Medical Association of Thailand 54, 466474.
Pholsena, K., Sayaseng, B., Hongvanthong, B. & Vanisaveth, V. (1991) The prevalence of helminth infection in Ban Nanin, Laos. Southeast Asian Journal of Tropical Medicine and Public Health 22, 137138.
Radomyos, P., Bunnag, D. & Harinasuta, T. (1984) Worms recovered in stool following praziquantel treatment. Arzneimittel-Forschung/Drug Research 34, 12151217.
Radomyos, P., Radomyos, B. & Tungtrongchitr, A. (1994) Multi-infection with helminths in adults from northeast Thailand as determined by post-treatment fecal examination of adult worms. Tropical Medicine and Parasitology 45, 133135.
Rim, H.J. (1982) Opisthorchiasis pp.109121 in Steele, J.H. (Eds) CRC handbook series in zoonoses, Section C: Parasitic zoonoses, Vol. III (trematode zoonoses). Boca Raton, Florida CRC Press.
Rim, H.J., Chai, J.Y., Min, D.Y., Cho, S.Y., Eom, K.S., Hong, S.J., Sohn, W.M., Yong, T.S., Deodato, G., Standgaard, H., Phommasack, B., Yun, C.Y. & Hoang, E.H. (2003) Prevalence of intestinal parasite infections on a national scale among primary schoolchildren in Laos. Parasitology Research 91, 267272.
Segal, D.B., Humphrey, J.M., Edwards, S.J. & Kirby, M.D. (1968) Parasites of man and domestic animals in Vietnam, Thailand, Laos, and Cambodia. Experimental Parasitology 23, 412464.
Scholtz, T., Ditrich, O. & Giboda, M. (1990) Larval stages of medically important flukes (Trematoda) from Vientiane Province, Laos. Part, I. metacercariae. Annales de Parasitologie Humaine et Comparée 65, 238243.
Sornmani, S., Pathammavong, O., Bunnag, T., Impand, P., Intarakhao, C. & Thirachantra, S. (1974) An epidemiological survey of human intestinal parasites in Vientiane, Laos. Southeast Asian Journal of Tropical Medicine and Public Health 5, 541546.
Tesana, S. Srisawangwonk, T. Kaewkes, S. Sithithaworn, P. Kanla, P. & Arunyanart, C. (1991) Eggshell morphology of the small eggs of human trematodes in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 22, 631636.
Yu, S.H. & Mott, K.E. (1994) Epidemiology and morbidity of food-borne intestinal trematode infections. Tropical Diseases Bulletin 91, R125R152.
Waikagul, J. (1991) Intestinal fluke infections in southeast Asia. Southeast Asian Journal of Tropical Medicine and Public Health 22, Suppl. 158162.
World Health Organization (1995) Control of foodborne trematode infections. WHO Technical Report Series No. 849, pp. 1157.
World Health Organization (2004) Report of Joint WHO/FAO Workshop on Food-borne trematode infections in Asia, Ha Noi, Vietnam, 26–28 November, 2002. WHO, WPRO, pp. 158.
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Journal of Helminthology
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