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Molecular and morphological characterization of Paurodontoides siddiqii n. sp. (Nematoda: Hexatylina, Sphaerulariidae) associated with bark samples of Pinus eldarica from western Iran

  • M. Esmaeili (a1), B. Golhasan (a1), W. Ye (a2) and R. Heydari (a1)


A new species of Paurodontoides, P. siddiqii n. sp., is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by a female 550–729 μm long, lip region continuous with body contour, stylet length 7.0–8.0 μm long or c. 1.0–1.2 times the lip region diameter, lateral fields with four smooth incisures, excretory pore at 85–125 μm from anterior end located at the base of the pharyngeal bulb or posterior to it, basal pharyngeal bulb with a short posterior extension projecting into the intestine, monodelphic–prodelphic reproductive system with prominent 19–22 μm long post-uterine sac, and elongate conoid tail with a filiform terminus. The new species is compared with two known species of the genus. It differs from the type species of the genus, P. linfordi, by having slightly shorter stylet, lateral field with smooth incisures, different position of the excretory pore, and absence of male. Compared to P. latus, the new species has a shorter body, shorter stylet, different position of the excretory pore, female tail shape and absence of male. The new species was also compared with close species of the genus Paurodontus because of lateral field marked with four lines, asymmetrical stylet knobs and absence of male. Molecular phylogenetic studies of the new species using partial sequences of 18S rDNA revealed that it forms a clade with a species of the genus Ficotylus. In phylogenetic analyses using partial sequences of the 28S rDNA D2-D3 domain, the new species formed a monophyletic group with a species of the genus Veleshkinema and Sphaerularia spp. (Sphaerulariinae).


Corresponding author

Author for correspondence: R. Heydari, E-mail:


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Andrássy, I (1976) Evolution as a Basis for the Systematization of Nematodes. London: Pitman Publishing.
Andrássy, I (2007) Free-Living Nematodes of Hungary, II (Nematoda Errantia). Budapest, Hungary: Hungarian Natural History Museum and Systematic Zoology Research Group of the Hungarian Academy of Sciences.
Arnold, TW (2010) Uninformative parameters and model selection using Akaike's information criterion. Journal of Wildlife Management 74, 11751178.
Chizhov, VN (2004) Entomopathogeneous nematodes from the suborder Hexatylina (Nematoda: Tylenchida). In MD, Sonin (ed.), Parasitic Nematodes of Plants and Insects. Moscow, Russia: Nauka, pp. 277293.
Das, VM and Shivaswamy, V (1980) Paurodontus brassicae n. sp. and Nothotylenchus singhi n. sp. from South India. Proceedings of the Indian Academy of Parasitology 1, 6265.
Davies, KA et al. (2009) Ficotylus congestae gen. n., sp. n. (Anguinata), from Ficus congesta (Moraceae) sycones in Australia. Nematology 11, 6375.
De Grisse, AT (1969) Redescription ou modifications de quelques techniques utiliséesdans l'étude des nématodes phytoparasitaires. Mededelingen FaculteitLandbouwwetenschappen Rijksuniversiteit Gent 34, 351369.
De Ley, P et al. (1999) Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591612.
Esmaeili, M, Heydari, R and Ye, W (2016a) Paurodontella parapitica n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Kermanshah Province, Western Iran. Journal of Nematology 48, 109115.
Esmaeili, M, Heydari, R and Ye, W (2016b) Molecular and morphological characterisation of Paurodontella persica n. sp. (Hexatylina: Sphaerulariidae) from soil in Iran. Nematology 19, 5768.
Esmaeili, M, Heydari, R and Ye, W (2017) Description of a new anguinid nematode, Nothotylenchus phoenixae n. sp. (Nematoda: Anguinidae) associated with palm date trees and its phylogenetic relations within the family Anguinidae. Journal of Nematology 49, 268275.
Fortuner, R and Raski, DJ (1987) A review of Neotylenchoidea Thorne, 1941 (Nemata: Tylenchida). Revue de Nématologie 10, 257267.
Geraert, E, Raski, DJ and Choi, YE (1985) A study of Stictylus intermedius n. comb. with a review of the genus (Nematoda: Tylenchida). Nematologica 30, 161171.
Giblin-Davis, RM et al. (2014) Ficotylus laselvae n. sp. (Tylenchomorpha: Anguinidae) associated with Ficus colubrinae in Costa Rica. Nematology 16, 11391151.
Golhasan, B, Heydari, R and Miraeiz, E (2016) Description of Paurodontella iranica (Nematoda: Sphaerulariidae) from Iran. Annales Zoologici 66, 125130.
Handoo, ZA et al. (2010) Two new species of Paurodontella Husain & Khan, 1968 (Nematoda: Sphaerulariidae) associated with wheat and a diagnostic compendium to the genus. Nematology 12, 181192.
Hechler, HC (1962) The description, feeding habits, and life history of Neotylenchus linfordi n.sp.; a mycophagous nematode. Proceedings of the Helminthological Society of Washington 29, 1927.
Holterman, M et al. (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 17921800.
Huelsenbeck, JP and Ronquist, F (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 17541755.
Husain, SI and Khan, AM (1965) A new genus and six new species of nematodes from India belonging in the family Neotylenchidae with an amendation of the subfamily Ecphyadophorinae. Proceedings of the Helminthological Society of Washington 32, 715.
Jairajpuri, MS and Siddiqi, MR (1969) Paurodontoides n. gen. (Paurodontidae) with an outline classification of Neotylenchoidea n. rank. Nematologica 15, 287288.
Koshel, EI et al. (2014) Phylogenetic analysis of entomoparasitic nematodes, potential control agents of flea populations in natural foci of plague. BioMed Research International 2014, 135218.
Larget, B and Simon, DL (1999) Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750759.
Miraeiz, E et al. (2015) Molecular and morphological characterization of Veleshkinema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran. Zootaxa 4000, 531546.
Miraeiz, E, Heydari, R and Golhasan, B (2017) A new and a known species of Deladenus Thorne, 1941 (Nematoda: Neotylenchidae) from Iran, with an updated species checklist of the genus. Acta Zoologica Bulgarica 69, 307316.
Mobasseri, M, Pedram, M and Pourjam, E (2017) A new species of the rare genus Anguillonema Fuchs, 1938 (Nematoda: Hexatylina, Sphaerularioidea) with its molecular phylogenetic study. Journal of Nematology 49, 286294.
Nunn, GB (1992) Nematode Molecular Evolution (Doctoral dissertation). University of Nottingham, Nottingham, UK.
Posada, D and Crandall, KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817818.
Siddiqi, MR (1961) A new species of the genus Paurodontus Thorne, 1941 (Nematoda: Neotylenchidae) from India. Proceedings of the Helminthological Society of Washington 28, 213215.
Siddiqi, MR (1986) Tylenchida: Parasites of Plants and Insects. Slough, UK: Commonwealth Agricultural Bureaux, Farnham Royal.
Siddiqi, MR (2000) Tylenchida: Parasites of Plants and Insects, 2nd edition. Wallingford, UK: CABI Publishing.
Sumenkova, NI (1975) Nematodes of Plants and Soil. Neotylenchoidea. Moscow, USSR: Nauka.
Thorne, G (1934) The sugar-beet nematode and other indigenous nemic parasites of shadscale. Journal of Agriculture Reseach 51, 509514.
Thorne, G (1949) On the classification of the Tylenchida, new order (Nematoda, Phasmidia). Proceedings of the Helminthological Society of Washington 16, 3773.
Whitehead, AG and Hemming, JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55, 2538.
Yaghoubi, A et al. (2014) Description of Abursanema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran and its phylogenetic relationships. Zootaxa 3826, 301314.
Yaghoubi, A et al. (2018) Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran. Nematology 20, 471482.
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Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
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