Skip to main content
×
Home
    • Aa
    • Aa
  • Access
  • Cited by 3
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Cavana, Paola Bensignor, Emmanuel Blot, Stéphane Carlus, Marine Chermette, René Crosaz, Odile Grimm, Felix Hurion, Murielle Jeandel, Aurélien and Polack, Bruno 2015. Nematode dermatitis due toAngiostrongylus vasoruminfection in a dog. Veterinary Dermatology, Vol. 26, Issue. 4, p. 293.


    Hu, Wei Wu, Sheng Yu, Xingang Abullahi, Auwalu Yusuf Song, Meiran Tan, Liping Wang, Zhen Jiang, Biao and Li, Guoqing 2015. A Multiplex PCR for Simultaneous Detection of Three Zoonotic ParasitesAncylostoma ceylanicum,A. caninum,andGiardia lambliaAssemblage A. BioMed Research International, Vol. 2015, p. 1.


    Liu, Yuanjia Zheng, Guochao Alsarakibi, Muhamd Zhang, Xinheng Hu, Wei Lin, Liqin Tan, Liping Luo, Qin Lu, Pengyun and Li, Guoqing 2014. The Zoonotic Risk ofAncylostoma ceylanicumIsolated from Stray Dogs and Cats in Guangzhou, South China. BioMed Research International, Vol. 2014, p. 1.


    ×

Molecular identification of hookworms in stray and shelter dogs from Guangzhou city, China using ITS sequences

  • Y.J. Liu (a1), G.C. Zheng (a1), P. Zhang (a1), M. Alsarakibi (a1), X.H. Zhang (a1), Y.W. Li (a1), T. Liu (a1), S.N. Ren (a1), Z.X. Chen (a1), Y.L. Liu (a1), S.J. Li (a1) and G.Q. Li (a1)
  • DOI: http://dx.doi.org/10.1017/S0022149X13000783
  • Published online: 27 November 2013
Abstract
Abstract

Canine hookworm infections are endemic worldwide, with zoonotic transmission representing a potentially significant public health concern. This study aimed to investigate hookworm infection and identify the prevalent species from stray and shelter dogs in Guangzhou city, southern China by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) based on internal transcribed spacer (ITS) sequences. From March 2011 to July 2012, fresh faecal samples from a total of 254 dogs were obtained from five locations, namely Conghua, Baiyun, Liwan, Haizhu and Panyu, in Guangzhou. These samples were screened for the presence of hookworm eggs using light microscopy, with an overall prevalence of 29.53% being recorded. The highest prevalence of 45.28% was found in suburban dogs from Conghua compared with lower values recorded in urban dogs in Haizhu (21.43%), Baiyun (18.97%), Panyu (18.18%) and Liwan (15%). The prevalence in stray dogs was significantly higher than that in shelter dogs. PCR-RFLP analysis showed that 57.33% were detected as single hookworm infections with Ancyclostoma caninum, and 22.67% as A. ceylanicum, while 20% were mixed infections. This suggests that high prevalences of both hookworm species in stray and shelter dogs in China pose a potential risk of transmission from pet dogs to humans.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Molecular identification of hookworms in stray and shelter dogs from Guangzhou city, China using ITS sequences
      Your Kindle email address
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Molecular identification of hookworms in stray and shelter dogs from Guangzhou city, China using ITS sequences
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Molecular identification of hookworms in stray and shelter dogs from Guangzhou city, China using ITS sequences
      Available formats
      ×
Copyright
Corresponding author
*E-mail: gqli@scau.edu.cn
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

R.C. Anderson (2000) Nematode parasites of vertebrates: Their development and transmission. 2nd edn.650 pp. Guilford, CABI.

D.D. Bowman , C.M. Hendrix , D.S. Lindsay & S.C. Barr (2002) Feline clinical parasitology. 1st edn.469 pp.Oxford, Wiley-Blackwell.

D.D. Bowman , S.P. Montgomery , A.M. Zajac , M.L. Eberhard & K.R. Kazacos (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends in Parasitology 26, 162167.

J. Choo , E. Pang & P. Prociv (2000) Hookworms in dogs of Kuching, Sarawak (north Borneo). Transactions of the Royal Society of Tropical Medicine and Hygiene 94, 2122.

J. Croese , A. Loukas , J. Opdebeeck & P. Prociv (1994) Occult enteric infection by Ancylostoma caninum: a previously unrecognized zoonosis. Gastroenterology 106, 312.

Y.C. Hsu & J.T. Lin (2012) Images in clinical medicine. Intestinal infestation with Ancylostoma ceylanicum. New England Journal of Medicine 366, e20.

A. Loukas , J. Croese , J. Opdebeeck & P. Prociv (1992) Detection of antibodies to secretions of Ancylostoma caninum in human eosinophilic enteritis. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 650653.

M.A. Mahdy , Y.A. Lim , R. Ngui , M.S. Fatimah , S.H. Choy , N.J. Yap , H.M. Al-Mekhlafi , J. Ibrahim & J. Surin (2012) Prevalence and zoonotic potential of canine hookworms in Malaysia. Parasites & Vectors 5, 88.

R. Ngui , S. Ishak , C.S. Chuen , R. Mahmud & Y.A. Lim (2011) Prevalence and risk factors of intestinal parasitism in rural and remote West Malaysia. PLoS Neglected Tropical Diseases 5, e974.

R. Ngui , Y.A. Lim , R. Traub , R. Mahmud & M.S. Mistam (2012) Epidemiological and genetic data supporting the transmission of Ancylostoma ceylanicum among human and domestic animals. PLoS Neglected Tropical Diseases 6, e1522.

C.S. Palmer , R.J. Traub , I.D. Robertson , R.P. Hobbs , A. Elliot , L. While , R. Rees & R.C. Thompson (2007) The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum. Veterinary Parasitology 145, 304313.

M. Sato , S. Sanguankiat , T. Yoonuan , T. Pongvongsa , M. Keomoungkhoun , I. Phimmayoi , B. Boupa , K. Moji & J. Waikagul (2010) Copro-molecular identification of infections with hookworm eggs in rural Lao PDR. Transactions of the Royal Society of Tropical Medicine and Hygiene 104, 617622.

L.M. Silva , R.R. Miranda , H.A. Santos & E.M. Rabelo (2006) Differential diagnosis of dog hookworms based on PCR-RFLP from the ITS region of their rDNA. Veterinary Parasitology 140, 373377.

R.C. Thompson & J.V. Conlan (2011) Emerging issues and parasite zoonoses in the SE Asian and Australasian region. Veterinary Parasitology 181, 6973.

J.D. Thompson , T.J. Gibson , F. Plewniak , F. Jeanmougin & D.G. Higgins (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.

R.J. Traub , I.D. Robertson , P. Irwin , N. Mencke & R.C. Thompson (2004) Application of a species-specific PCR-RFLP to identify Ancylostoma eggs directly from canine faeces. Veterinary Parasitology 123, 245255.

R.J. Traub , T. Inpankaew , C. Sutthikornchai , Y. Sukthana & R.C. Thompson (2008) PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by Ancylostoma ceylanicum in temple communities in Bangkok. Veterinary Parasitology 155, 6773.

C.C. Velasquez & B.C. Cabrera (1968) Ancylostoma ceylanicum (Looss, 1911) in a Filipino woman. Journal of Parasitology 54, 430431.

C.R. Wang , J.H. Qiu , J.P. Zhao , L.M. Xu , W.C. Yu & X.Q. Zhu (2006) Prevalence of helminthes in adult dogs in Heilongjiang Province, the People's Republic of China. Parasitology Research 99, 627630.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Helminthology
  • ISSN: 0022-149X
  • EISSN: 1475-2697
  • URL: /core/journals/journal-of-helminthology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×