Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-18T09:19:37.662Z Has data issue: false hasContentIssue false
  • English
  • Français

Histological analysis of the effects of anti-adhesive haemostatic agents on the middle ear of the guinea pig

Published online by Cambridge University Press:  29 September 2014

E Kulduk ,
E Eren ,
FK Soy ,
R Dundar ,
M Aslan ,
MS Basoglu ,
F Simsek  and
H Katilmis
E Kulduk*
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
E Eren
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
FK Soy
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
R Dundar
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
M Aslan
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
MS Basoglu
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
F Simsek
Affiliation:
Department of Histology and Embryology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
H Katilmis
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
*
Address for correspondence: Dr Erkan Kulduk, Department of Otolaryngology, Mardin State Hospital, Mardin, Turkey Fax: +90 232 243 1530 E-mail: erkankulduk@yahoo.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Introduction:

Adhesion formation is a frequent and serious post-operative problem in ENT surgery. This study assessed the effect of two anti-adhesive haemostatic agents on an experimental guinea pig model.

Materials and methods:

The middle-ear mucosa of 14 guinea pigs was exposed to surgical trauma. After surgery, Arista™ AH was injected into the right middle ear of seven animals, while Ankaferd Blood Stopper was injected into the right middle ear of the other seven animals. The left ears were left untreated and regarded as the control group. The three groups were compared by histological examination at post-operative week 4.

Results:

In each of the three groups, consolidation of the lamina propria and epithelium mucosae, increments in the number of active fibroblasts, collagen fibrils and inflammatory cells, and increased vascular dilation were observed on haematoxylin and eosin-stained sections, and were more prominent in the control and Ankaferd Blood Stopper groups. Epithelial thickness and capillary vasodilation were significantly lower in the Arista™ AH group compared with the control and Ankaferd Blood Stopper groups (p < 0.008).

Conclusion:

Arista™ AH may prevent the formation of adhesions in middle-ear surgery. Further experimental studies are required to determine its ototoxic potential.

Keywords

Guinea PigsEar, MiddleSurgery-Induced Tissue Adhesions
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 128 , Issue 10 , October 2014 , pp. 885 - 891
Copyright
Copyright © JLO (1984) Limited 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Yener, A, Bardakçı, Y, Iriz, E, Özoğul, C, Yener, N, Bayram, H et al. Intraoperative changes in the pericardium: a study via electron microscope [in Turkish]. Türk göğüs kalp damar cerrahisi dergisi 2011;19:576–85Google Scholar
2Hellebrekers, BW, Trimbos-Kemper, GC, van Blitterswijk, CA, Bakkum, EA, Trimbos, JB. Effects of five different barrier materials on postsurgical adhesion formation in the rat. Hum Reprod 2000;15:1358–63CrossRefGoogle ScholarPubMed
3Hellström, S, Salén, B, Stenfors, LE. Absorbable gelatin sponge (Gelfoam) in otosurgery: one cause of undesirable postoperative results? Acta Otolaryngol 1983;96:269–75CrossRefGoogle ScholarPubMed
4Jang, CH, Park, H, Cho, YB, Choi, CH. The effect of anti-adhesive packing agents in the middle ear of guinea pig. Int J Pediatr Otorhinolaryngol 2008;72:1603–8Google Scholar
5Dogru, S, Haholu, A, Gungor, A, Kucukodaci, Z, Cincik, H, Ozdemir, T et al. Histologic analysis of the effects of three different support materials within rat middle ear. Otolaryngol Head Neck Surg 2009;140:177–82CrossRefGoogle ScholarPubMed
6diZerega, GS, Tulandi, T. Prevention of intra-abdominal adhesions in gynaecological surgery. Reprod Biomed Online 2008;17:303–6Google Scholar
7Reed, KL, Heydrick, SJ, Aarons, CB, Prushik, S, Gower, AC, Stucchi, AF et al. A neurokinin-1 receptor antagonist that reduces intra-abdominal adhesion formation decreases oxidative stress in the peritoneum. Am J Physiol Gastrointest Liver Physiol 2007;293:544–51CrossRefGoogle ScholarPubMed
8Firat, HC, Ozdemir, O, Kosar, A, Goker, H, Haznedaroglu, IC. Annual Review of Ankaferd 08–09. Istanbul: Naviga Publications, 2009;13–19Google Scholar
9Teker, AM, Korkut, AY, Gedikli, O, Kahya, V. Prospective, controlled clinical trial of Ankaferd Blood Stopper in children undergoing tonsillectomy. Int J Pediatr Otorhinolaryngol 2009;73:1742–5CrossRefGoogle ScholarPubMed
10Guler, M, Maralcan, G, Kul, S, Baskonus, I, Yılmaz, M. The efficacy of Ankaferd Blood Stopper for the management of bleeding following total thyroidectomy. J Invest Surg 2011;24:205–10Google Scholar
11Cömert, M, Karakaya, K, Barut, F, Karadeniz Çakmak, G, Uçan, HB, Gültekin, FA et al. Does intraabdominal use of Ankaferd Blood Stopper cause increased intraperitoneal adhesions? Ulus Travma Acil Cerrahi Derg 2010;16:383–9Google ScholarPubMed
12Odabaş, ME, Ertük, M, Çinar, Ç, Tüzüner, T, Tulunoğlu, Ö. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Patol Oral Cir Bucal 2011;16:e584–7CrossRefGoogle ScholarPubMed
13Emmez, H, Tonge, M, Tokgoz, N, Durdag, E, Gonul, I, Ceviker, N. Radiological and histopathological comparison of microporous polysaccharide hemospheres and oxidized regenerated cellulose in the rabbit brain: a study of efficacy and safety. Turk Neurosurg 2010:20;485–91Google Scholar
14Antisdel, JL, Janney, CG, Long, JP, Sindwani, R. Hemostatic agent microporous polysaccharide hemospheres (MPH) does not affect healing or intact sinus mucosa. Laryngoscope 2008;118:1265–9CrossRefGoogle ScholarPubMed
15Hoffmann, NE, Siddiqui, SA, Agarwal, S, McKellar, SH, Kurtz, HJ, Gettman, MT et al. Choice of hemostatic agent influences adhesion formation in a rat cecal adhesion model. J Surg Res 2009;155:77–81Google Scholar
16Sindwani, R. Use of novel hemostatic powder MPH for endoscopic sinus surgery: initial impressions. Otolaryngol Head Neck Surg 2009;140:262–3CrossRefGoogle ScholarPubMed