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Single dose intratympanic mesna application inhibits propylene glycol induced cholesteatoma formation

  • O Ismi (a1), Y Y Karabulut (a2), K K Bal (a1), Y Vayisoglu (a1) and M Unal (a1)...
Abstract
Abstract Objective:

Mesna (i.e. sodium 2-mercaptoethanesulfonate; C2H5NaO3S2) has been used in otological surgery such as cholesteatoma dissection and tympanic membrane lateralisation in atelectatic ears. However, this study aimed to investigate its effect on cholesteatoma formation.

Methods:

A total of 20 Wistar rats were divided into two groups of 10 animals. The right and left ears of control animals were treated with saline (saline control group; n = 10 ears) and propylene glycol plus saline (propylene glycol control group; n = 10 ears), respectively. In the mesna group, both ears were treated with propylene glycol plus mesna (n = 20 ears). On days 1, 8 and 15, the saline control group had intratympanic injections of 0.2 ml saline and the propylene glycol control and mesna groups had intratympanic injections of 0.2 ml 100 per cent propylene glycol. On day 22, the propylene glycol control group had a single intratympanic injection of 0.2 ml saline and the mesna group had a single intratympanic injection of 10 per cent mesna. Animals were killed 12 weeks after the last injection and the temporal bones were sent for histopathological evaluation.

Results:

The cholesteatoma formation rate was 88 per cent in the propylene glycol control group, but was significantly lower in the mesna group (p = 0.01). There were no significant differences in granulation tissue formation (p = 0.498), cyst formation in the bulla (p = 0.381), fibrosis (p = 0.072) and epithelial hyperplasia (p = 0.081) among experimental groups.

Conclusion:

Intratympanic propylene glycol administration is an effective method of promoting experimental cholesteatoma formation. Administration of a single dose of intratympanic mesna inhibited cholesteatoma formation in an animal model.

Copyright
Corresponding author
Address for correspondence: Dr O Ismi, Mersin Üniversitesi Tıp Fakültesi Kulak Burun Boğaz A D, Çiftlikköy, Mezitli, Mersin, Turkey E-mail: dronurismi@gmail.com
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1 H Sudhoff , Y Liebehenz , J Aschenbrenner , J Jung , H Hildmann , S. Dazert A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation. Laryngoscope 2003;113:1022–6

2 G Choufani , N Roper , C Delbrouck , S Hassid , HJ. Gabius Animal model for cholesteatoma induced in the gerbil: will the profiles of differentiation/growth-regulatory markers be similar to the clinical situation? Laryngoscope 2007;117:706–11

3 CL Kuo , Etiopathogenesis of acquired cholesteatoma: prominent theories and recent advances in biomolecular research. Laryngoscope 2015;125:234–40

4 O Ismi , M. Unal Experimental models of cholesteatoma: a review. World J Otorhinolaryngol 2014;4:23–7

5 CG Wright , LL Bird , WL. Meyerhoff Effect of 5-fluorouracil in cholesteatoma development in an animal model. Am J Otolaryngol 1991;12:133–8

6 ML Antunes , Y Fukuda , O Penido Nde , R. Ferreira Effect of trans-retinoic acid in the inhibition of cholesteatoma in guinea pigs. Braz J Otorhinolaryngol 2008;74:5360

7 AA Melo , SS Caldas Neto , FS Leão , AJC. Campos Effect of intratympanic mitomycin C on the development of cholesteatoma and otitis media in rats. J Laryngol Otol 2013;127:359–63

8 L Sennaroglu , A Ozkul , G Gedikoglu , T. Ergin Effect of intratympanic steroid application on the development of experimental cholesteatoma. Laryngoscope 1998;108:543–7

10 SJ White , CG Wright , KS Robinson , ML. Meyerhoff Effect of topical hyaluronic acid on experimental cholesteatoma. Am J Otolaryngol 1995;16:312–18

11 PH Pownell , CG Wright , KS Robinson , WL. Meyerhoff The effect of cyclophosphamide on development of experimental cholesteatoma. Arch Otolaryngol Head Neck Surg 1994;120:1114–16

13 M Yilmaz , N Goksu , I Bayramoglu , YA. Bayazit Practical use of MESNA in atelectatic ears and adhesive otitis media. ORL J Otorhinolaryngol Relat Spec 2006;68:195–8

14 MT Kalcioglu , MT Cicek , T Bayindir , OI. Ozdamar Effectiveness of MESNA on the success of cholesteatoma surgery. Am J Otolaryngol 2014;35:357–61

15 M Casale , A Di Martino , F Salvinelli , M Trombetta , V. Denaro MESNA for chemically assisted tissue dissection. Expert Opin Investig Drugs 2010;19:699707

16 J Song , L Liu , L Li , J Liu , E Song , Y. Song Protective effects of lipoic acid and mesna on cyclophosphamide-induced haemorrhagic cystitis in mice. Cell Biochem Funct 2014;32:125–32

17 V Vincenti , M Mondain , E Pasanisi , F Piazza , JL Puel , S Bacciu , Cochlear effects of Mesna application into middle ear. Ann N Y Acad Sci 1999;884:425–32

18 MP Van Spaendonck , JP Timmermans , J Claes , W Scheuermann , FL Wuyts , PH. Van De Heyning Single ototopical application of mesna has no ototoxic effects on guinea pig cochlear hair cells: a morphological study. Acta Otolaryngol 1999;119:685–9

20 V Vincenti , J Magnan , MS Saccardi , C. Zini Chemically assisted dissection by means of Mesna in cholesteatoma surgery. Otol Neurotol 2014;35:1819–24

21 MA Jove , L Vassalli , W Raslan , EL. Aoolebaum The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears. Am J Otolaryngol 1990;11:59

24 L. Louw Acquired cholesteatoma pathogenesis: stepwise explanations. J Laryngol Otol 2010;124:587–93

25 G Eskiizmir , AV Yuceturk , E Onur , A Var , P. Temiz The imbalance of enzymatic antioxidants in cholesteatoma. Acta Otolaryngol 2009;129:1187–91

26 H Sudhoff , M. Tos Pathogenesis of sinus cholesteatoma. Eur Arch Otorhinolaryngol 2007;264:1137–43

27 X Feng , PA. Coulombe A role of disulfide bonding in keratin intermediate filaments organization and dynamics in skin keratinocytes. J Cell Biol 2015;209:5972

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The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
  • URL: /core/journals/journal-of-laryngology-and-otology
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